Issue
The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.
Development of a loop-mediated isothermal amplification assay for rapid detection of Burkholderia mallei
Corresponding Author(s) : S Mirzai
Cellular and Molecular Biology,
Vol. 62 No. 10: Issue 10
Abstract
The present study was conducted to establish a Loop-mediated isothermal amplification (LAMP) technique for the rapid detection of B. mallei the etiologic agent of glanders, a highly contagious disease of equines. A set of six specific primers targeting integrase gene cluster were designed for the LAMP test. The reaction was optimized using different temperatures and time intervals. The specificity of the assay was evaluated using DNA from B.pseudomallei and Pseudomonas aeruginosa. The LAMP products were analyzed both visually and under UV light after electrophoresis. The optimized conditions were found to be at 63ºC for 60 min. The assay showed high specificity and sensitivity. It was concluded that the established LAMP assay is a rapid, sensitive and practical tool for detection of B. mallei and early diagnosis of glanders.
Keywords
Glanders
identification
Burkholderia mallei
LAMP.
Mirzai, S., Safi, S., Mossavari, N., Afshar, D., & Bolourchian, M. (2016). Development of a loop-mediated isothermal amplification assay for rapid detection of Burkholderia mallei. Cellular and Molecular Biology, 62(10), 32–32. Retrieved from https://mail.cellmolbiol.org/index.php/CMB/article/view/602
Download Citation
Endnote/Zotero/Mendeley (RIS)BibTeX