The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.
Characterization of native and denatured ricin using MALDI-TOF/MS
Corresponding Author(s) : O. Kumar
omkumar63@rediffmail.com
Cellular and Molecular Biology,
Vol. 56 No. 3: General Papers
Abstract
Ricin is a toxic protein present in the seeds of castor bean plant. It can be inactivated by heat; therefore characterization of denatured ricin is essential to differentiate it from native ricin and to avoid any ambiguity in its identification. In this study, potential of mass spectrometry using MALDI-TOF/MS has been exploited to investigate the effects of heat treatment on ricin and spiked food matrices. The molecular weights of ricin, ricin A (A1 and A2) and B chain were found to be 62.8 kDa, 31.2 kDa, 32.5 kDa and 32 kDa respectively. The mass spectrum revealed a polypeptide chain of 11.1 kDa for denatured ricin. The peptide mass fingerprinting showed 24 peptides, six were common both in native and denatured ricin. The differentiating peptide at position 294-318 (m/z 934.533) was observed only in denatured ricin. The three selected marker peptides m/z 1013.6, 1310.7, 1728.9 are chosen for identification of ricin inactivated by heat in spiked apple juice and milk samples by immunocapture analysis. There is always a probability of denatured non- toxic ricin being confused with native (toxic) ricin to create unnecessary panic. Keeping this probability in mind, our study will be of immense value in minimising such risk.
Keywords
Ricin
denatured
ambiguity
MALDI-TOF/MS
peptide mass fingerprinting
marker
immunocapture.
Sehgal, P., Rao, M. K., Kumar, O., & Vijayaraghavan, R. (2010). Characterization of native and denatured ricin using MALDI-TOF/MS. Cellular and Molecular Biology, 56(3), 1385–99. Retrieved from https://mail.cellmolbiol.org/index.php/CMB/article/view/985
Download Citation
Endnote/Zotero/Mendeley (RIS)BibTeX