Antioxidant effect of curcumin against exposure to malathion in Cyprinus carpio

S. Mişe Yonar; M. E. Yonar; M. Åž. Ural

doi:10.14715/cmb/2017.63.3.13

Issue

Vol. 63 No. 3: Issue 3

Issue Published : May 3, 2017

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Antioxidant effect of curcumin against exposure to malathion in Cyprinus carpio

https://doi.org/10.14715/cmb/2017.63.3.13

S. Mişe Yonar

Department of Aquaculture and Fish Diseases, Firat University, Fisheries Faculty, Elazig, Turkey

M. E. Yonar

Department of Aquaculture and Fish Diseases, Firat University, Fisheries Faculty, Elazig, Turkey

M. Åž. Ural

College of Keban, Fisheries Programme, Firat University, Elazig, Turkey

Corresponding Author(s) : M. Åž. Ural

msural@firat.edu.tr

Cellular and Molecular Biology, Vol. 63 No. 3: Issue 3
Article Published : March 31, 2017

Abstract

The aim of this study was to determine protective effects of curcumin on oxidant/antioxidant parameters in Cyprinus carpio exposed to malathion. The fish were exposed to two sublethal concentrations of malathion (0.5 and 1 mg/L), and curcumin (100 mg per kg of fish weight) was simultaneously administered for 14 days. Malondialdehyde level and superoxide dismutase, catalase, glutathione peroxidase, and glutathione-S-transferase activities were monitored in liver, kidney and gills, which were collected at the end of the experiment. The results revealed a significant increase in the malondialdehyde levels of the groups that were exposed to malathion. Also, malathion exposure caused a significant increase in superoxide dismutase, catalase, and glutathione-S-transferase activities and a significant decrease in glutathione peroxidase activity. Treatment with curcumin attenuated the malathion-induced oxidative stress by significantly decreasing the levels of malondialdehyde in the tissues. In addition, curcumin reversed the superoxide dismutase, catalase, glutathione peroxidase, and glutathione-S-transferase activities. In conclusion, this study demonstrated that malathion caused oxidative stress and negative alterations on the antioxidant enzyme activities of the fish. However, this toxic effect was neutralised by the administration of curcumin.

Keywords

Antioxidants Curcumin Fish Malathion Oxidative stress.

Mişe Yonar, S., Yonar, M. E., & Ural, M. Åž. (2017). Antioxidant effect of curcumin against exposure to malathion in Cyprinus carpio. Cellular and Molecular Biology, 63(3), 68–72. https://doi.org/10.14715/cmb/2017.63.3.13

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References

Zhang J, Shen H, Wang X, Wu J, Xue,Y. Effects of chronic exposure of 2,4-dichlorophenol on the antioxidant system in liver of freshwater fish Carassius auratus. Chemosphere 2004 55:167–74.
Peixoto F, Alves-Fernandes D, Santos D, Fontaínhas-Fernandes A. Toxicological effects of oxyfluorfen on oxidative stress enzymes in tilapia Oreochromis niloticus. Pestic Biochem Phys 2006 85:91–6.
Puangkaew J, Kiron V, Satoh S, Watanabe T. Antioxidant defense of rainbow trout (Oncorhynchus mykiss) in relation to dietary n-3 highly unsaturated fatty acids and vitamin E contents. Comp Biochem Physiol C 2005 140:187–96.
Mişe Yonar S, Ural MÅž, Silici S, Yonar ME. Malathion-induced changes in the haematological profile, the immune response, and the oxidative/antioxidant status of Cyprinus carpio carpio: Protective role of propolis. Ecotoxicol Environ Saf 2014 102:202–9
Kappus H. Lipid peroxidation: mechanisms, analysis, enzymology and biological relevance. In: Sies H (eds) Oxidative Stress; Oxidants and Antioxidants. Academic Press, London, 1985 p 273–310.
Kohen R, Nyska A. Oxidation of biological systems: oxidative stress phenomena, antioxidants, redox reactions, and methods for their quantification. Toxicol Pathol 2002 30:620–50.
Yonar M.E. Protective effect of lycopene on oxidative stress and antioxidant status in Cyprinus carpio during cypermethrin exposure. Environ Toxicol 2013 28(11): 609-16.
Aebi H. Catalase. In: Bergmeyer HU (eds) Methods in enzymatic analysis. Academic Press, New York, 1983, pp 276–286.
Van der Oost R, Beyer J, Vermeulen NP. Fish bioaccumulation and biomarkers in environmental risk assessment: a review. Environ Toxicol Pharmacol 2003 13: 57–149.
Mişe Yonar S, Yonar ME, Yöntürk Y, Pala A. Effect of ellagic acid on some haematological, immunological and antioxidant parameters of rainbow trout (Oncorhynchus mykiss). J Anim Physiol An N 2014 98: 936-41.
Bruce A, Freeman D, James C. Biology of diseases free radicals and tissue injury. Laboratory Invest 1982 47: 412–26.
Winston GW, Di Giulio RT. Prooxidant and antioxidant mechanisms in aquatic organisms. Aquat Toxicol 1991 19:137–161.
Kim JH, Dahms HU, Rhee JS, Lee YM, Lee J, Han KN, Lee JS. Expression profiles of seven glutathione S-transferase (GST) genes in cadmium-exposed river pufferfish (Takifugu obscurus). Comp Biochem Physiol C 2010 151: 99–106.
Köprücü K, Mişe Yonar S, Åžeker E. Effects of cypermethrin on antioxidant status, oxidative stress biomarkers, behavior, and mortality in the freshwater mussel Unio elongatulus eucirrus. Fisheries Sci 2010 76(6): 1007-1013.
Lushchak VI. Environmentally induced oxidative stress in aquatic animals. Aquat Toxicol 2011 101(1): 13–30.
Monteiro DA, Alves de Almeida J, Rantin AFT, Kalinin L. Oxidative stress biomarkers in the freshwater characid ï¬sh, Brycon cephalus, exposed to organophosphorus insecticide Folisuper 600 (methyl parathion). Comp Biochem Physiol C 2006 143: 141–9.
Huculeci R, Dinu D, Staicu AC, Munteanu MC, Costache M, Dinischiotu A. Malathion-induced alteration of the antioxidant defence system in kidney, gill, and intestine of Carassius auratus gibelio. Environ Toxicol 2009 24: 523–30.
Mişe Yonar S. Toxic effects of malathion in carp, Cyprinus carpio carpio: Protective role of lycopene. Ecotoxicol Environ Saf 2013 97: 223–9.
Sweilum MA. Effect of sublethal toxicity of some pesticides on growth parameters, haematological properties and total production of Nile tilapia (Oreochromis niloticus L.) and water quality of ponds. Aqua Res 2006 37: 1079–89.
Venkataramana GV, Rani PN, Murthy PS. Impact of malathion on the biochemical parameters of gobiid fish, Glossogobius giuris (Ham). J Environ Biol 2006 27: 119–22.
Thayyullathil F, Chathoth S, Hago A, Patel M, Galadari S. Rapid reactive oxygen species (ROS) generation induced by curcumin leads to caspase-dependent and -independent apoptosis in L929 cells. Free Radical Bio Med 2008 45: 1403–12.
Rak S, Čimbora-Zovko T, Gajski G, Dubravćić K, Domijan AM, Delaš I, Garaj-Vrhovac V, Batinić D, Sorić J, Osmak M. Carboplatin resistant human laryngeal carcinoma cells are cross resistant to curcumin due to reduced curcumin accumulation. Toxicol in Vitro 2013 27:523–32.
Chan WH, Wu HY, Chang WH. Dosage effects of curcumin on cell death types in a human osteoblast cell line. Food Chem Toxicol 2006 44: 1362–71.
Ortiz-Ortiz MA, Morán JM, Bravosanpedro JM, González-Polo RA, Niso-Santano M, Anantharam V, Kanthasamy AG, Soler G, Fuentes JM. Curcumin enhances paraquat-induced apoptosis of N27 mesencephalic cells via the generation of reactive oxygen species. NeuroToxicology 2009 30: 1008–18.
Kaur K, Dhawan A. Variable sensitivity of Cyprinus carpio eggs, larvae, and fry to pesticides. B Environ Contam Tox 1993 50(4): 593–99.
Yonar ME, Mişe Yonar S, Pala A, Silici S, Saglam N. Trichlorfon-induced haematological and biochemical changes in Cyprinus carpio: ameliorative effect of propolis. Dis Aquat Organ 2015 114: 209-16.
Yonar ME, Mişe Yonar S, í‡oban MZ, Eroğlu M. Antioxidant effect of propolis against exposure to chromium in Cyprinus carpio. Environ Toxicol 2014 29(2): 155-64.
Mişe Yonar S, Sakin F, Yonar ME, Ispir íœ, Kirici M. Oxidative stress biomarkers of exposure to deltamethrin in rainbow trout fry (Oncorhynchus mykiss). Fresen Environ Bull 2011 20(8): 1931-35.
Sakin F, Ispir íœ, Mişe Yonar S, Yonar ME, Taysi R. Effect of short-term cypermethrin exposure on oxidant-antioxidant balance in the whole body of rainbow trout fry (Oncorhynchus mykiss). Fresen Environ Bull 2011 20(10a): 2806-9.
Placer ZA, Cushman L, Johnson BC. Estimation of products of lipid peroxidation (malonyl dialdehyde) in biological fluids. Anal Biochem 1966 16: 359–64.
Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clin Chem 1988 34: 497–500.
Beutler, E., Red cell metabolism. In: Beutler E (eds) A manual of Biochemical Methods. Grune and Stratton, New York, 1975, pp 71-73.
Habig WH, Pabst MJ, Jakoby WB. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem 1974 249: 7130–9.
Lowry OH, Rosenberough NJ, Farr AL, Randal RJ. Protein measurement with folin phenol reagent. J Biochem 1951 193: 265–75.
Mişe Yonar S, Yonar ME, Yöntürk Y. The effect of curcumin on some antioxidant parameters in rainbow trout (Oncorhynchus mykiss Walbaum, 1792). Firat Unv J Science 2014 26(1): 53-7.
Manju M, Akbarsha MA, Oommen OV. In vivo protective effect of dietary curcumin in ï¬sh Anabas testudineus (Bloch). Fish Physiol Biochem 2012 38: 309–18.
Ural MS, Yonar ME, Mişe Yonar S. Protective effect of ellagic acid on oxidative stress and antioxidant status in Cyprinus carpio during malathion exposure. Cell Mol Biol 2015 61(5): 58-63.

References

Zhang J, Shen H, Wang X, Wu J, Xue,Y. Effects of chronic exposure of 2,4-dichlorophenol on the antioxidant system in liver of freshwater fish Carassius auratus. Chemosphere 2004 55:167–74.

Peixoto F, Alves-Fernandes D, Santos D, Fontaínhas-Fernandes A. Toxicological effects of oxyfluorfen on oxidative stress enzymes in tilapia Oreochromis niloticus. Pestic Biochem Phys 2006 85:91–6.

Puangkaew J, Kiron V, Satoh S, Watanabe T. Antioxidant defense of rainbow trout (Oncorhynchus mykiss) in relation to dietary n-3 highly unsaturated fatty acids and vitamin E contents. Comp Biochem Physiol C 2005 140:187–96.

Mişe Yonar S, Ural MÅž, Silici S, Yonar ME. Malathion-induced changes in the haematological profile, the immune response, and the oxidative/antioxidant status of Cyprinus carpio carpio: Protective role of propolis. Ecotoxicol Environ Saf 2014 102:202–9

Kappus H. Lipid peroxidation: mechanisms, analysis, enzymology and biological relevance. In: Sies H (eds) Oxidative Stress; Oxidants and Antioxidants. Academic Press, London, 1985 p 273–310.

Kohen R, Nyska A. Oxidation of biological systems: oxidative stress phenomena, antioxidants, redox reactions, and methods for their quantification. Toxicol Pathol 2002 30:620–50.

Yonar M.E. Protective effect of lycopene on oxidative stress and antioxidant status in Cyprinus carpio during cypermethrin exposure. Environ Toxicol 2013 28(11): 609-16.

Aebi H. Catalase. In: Bergmeyer HU (eds) Methods in enzymatic analysis. Academic Press, New York, 1983, pp 276–286.

Van der Oost R, Beyer J, Vermeulen NP. Fish bioaccumulation and biomarkers in environmental risk assessment: a review. Environ Toxicol Pharmacol 2003 13: 57–149.

Mişe Yonar S, Yonar ME, Yöntürk Y, Pala A. Effect of ellagic acid on some haematological, immunological and antioxidant parameters of rainbow trout (Oncorhynchus mykiss). J Anim Physiol An N 2014 98: 936-41.

Bruce A, Freeman D, James C. Biology of diseases free radicals and tissue injury. Laboratory Invest 1982 47: 412–26.

Winston GW, Di Giulio RT. Prooxidant and antioxidant mechanisms in aquatic organisms. Aquat Toxicol 1991 19:137–161.

Kim JH, Dahms HU, Rhee JS, Lee YM, Lee J, Han KN, Lee JS. Expression profiles of seven glutathione S-transferase (GST) genes in cadmium-exposed river pufferfish (Takifugu obscurus). Comp Biochem Physiol C 2010 151: 99–106.

Köprücü K, Mişe Yonar S, Åžeker E. Effects of cypermethrin on antioxidant status, oxidative stress biomarkers, behavior, and mortality in the freshwater mussel Unio elongatulus eucirrus. Fisheries Sci 2010 76(6): 1007-1013.

Lushchak VI. Environmentally induced oxidative stress in aquatic animals. Aquat Toxicol 2011 101(1): 13–30.

Monteiro DA, Alves de Almeida J, Rantin AFT, Kalinin L. Oxidative stress biomarkers in the freshwater characid ï¬sh, Brycon cephalus, exposed to organophosphorus insecticide Folisuper 600 (methyl parathion). Comp Biochem Physiol C 2006 143: 141–9.

Huculeci R, Dinu D, Staicu AC, Munteanu MC, Costache M, Dinischiotu A. Malathion-induced alteration of the antioxidant defence system in kidney, gill, and intestine of Carassius auratus gibelio. Environ Toxicol 2009 24: 523–30.

Mişe Yonar S. Toxic effects of malathion in carp, Cyprinus carpio carpio: Protective role of lycopene. Ecotoxicol Environ Saf 2013 97: 223–9.

Sweilum MA. Effect of sublethal toxicity of some pesticides on growth parameters, haematological properties and total production of Nile tilapia (Oreochromis niloticus L.) and water quality of ponds. Aqua Res 2006 37: 1079–89.

Venkataramana GV, Rani PN, Murthy PS. Impact of malathion on the biochemical parameters of gobiid fish, Glossogobius giuris (Ham). J Environ Biol 2006 27: 119–22.

Thayyullathil F, Chathoth S, Hago A, Patel M, Galadari S. Rapid reactive oxygen species (ROS) generation induced by curcumin leads to caspase-dependent and -independent apoptosis in L929 cells. Free Radical Bio Med 2008 45: 1403–12.

Rak S, Čimbora-Zovko T, Gajski G, Dubravćić K, Domijan AM, Delaš I, Garaj-Vrhovac V, Batinić D, Sorić J, Osmak M. Carboplatin resistant human laryngeal carcinoma cells are cross resistant to curcumin due to reduced curcumin accumulation. Toxicol in Vitro 2013 27:523–32.

Chan WH, Wu HY, Chang WH. Dosage effects of curcumin on cell death types in a human osteoblast cell line. Food Chem Toxicol 2006 44: 1362–71.

Ortiz-Ortiz MA, Morán JM, Bravosanpedro JM, González-Polo RA, Niso-Santano M, Anantharam V, Kanthasamy AG, Soler G, Fuentes JM. Curcumin enhances paraquat-induced apoptosis of N27 mesencephalic cells via the generation of reactive oxygen species. NeuroToxicology 2009 30: 1008–18.

Kaur K, Dhawan A. Variable sensitivity of Cyprinus carpio eggs, larvae, and fry to pesticides. B Environ Contam Tox 1993 50(4): 593–99.

Yonar ME, Mişe Yonar S, Pala A, Silici S, Saglam N. Trichlorfon-induced haematological and biochemical changes in Cyprinus carpio: ameliorative effect of propolis. Dis Aquat Organ 2015 114: 209-16.

Yonar ME, Mişe Yonar S, í‡oban MZ, Eroğlu M. Antioxidant effect of propolis against exposure to chromium in Cyprinus carpio. Environ Toxicol 2014 29(2): 155-64.

Mişe Yonar S, Sakin F, Yonar ME, Ispir íœ, Kirici M. Oxidative stress biomarkers of exposure to deltamethrin in rainbow trout fry (Oncorhynchus mykiss). Fresen Environ Bull 2011 20(8): 1931-35.

Sakin F, Ispir íœ, Mişe Yonar S, Yonar ME, Taysi R. Effect of short-term cypermethrin exposure on oxidant-antioxidant balance in the whole body of rainbow trout fry (Oncorhynchus mykiss). Fresen Environ Bull 2011 20(10a): 2806-9.

Placer ZA, Cushman L, Johnson BC. Estimation of products of lipid peroxidation (malonyl dialdehyde) in biological fluids. Anal Biochem 1966 16: 359–64.

Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clin Chem 1988 34: 497–500.

Beutler, E., Red cell metabolism. In: Beutler E (eds) A manual of Biochemical Methods. Grune and Stratton, New York, 1975, pp 71-73.

Habig WH, Pabst MJ, Jakoby WB. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem 1974 249: 7130–9.

Lowry OH, Rosenberough NJ, Farr AL, Randal RJ. Protein measurement with folin phenol reagent. J Biochem 1951 193: 265–75.

Mişe Yonar S, Yonar ME, Yöntürk Y. The effect of curcumin on some antioxidant parameters in rainbow trout (Oncorhynchus mykiss Walbaum, 1792). Firat Unv J Science 2014 26(1): 53-7.

Manju M, Akbarsha MA, Oommen OV. In vivo protective effect of dietary curcumin in ï¬sh Anabas testudineus (Bloch). Fish Physiol Biochem 2012 38: 309–18.

Ural MS, Yonar ME, Mişe Yonar S. Protective effect of ellagic acid on oxidative stress and antioxidant status in Cyprinus carpio during malathion exposure. Cell Mol Biol 2015 61(5): 58-63.

Author biographies is not available.