Determination of inhibitory activities of enzymes, related to Alzheimer's disease and diabetes mellitus of plane tree (Platanus orientalis L.) extracts and their antioxidant, antimicrobial and anticancer activities

Esra Ucar; Nuraniye Eruygur; Mehmet Atas; Merve Ergul; Mustafa Ergul; Fazli Sozmen

doi:10.14715/cmb/2018.64.11.3

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Vol. 64 No. 11: Issue 11

Issue Published : August 31, 2018

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Determination of inhibitory activities of enzymes, related to Alzheimer's disease and diabetes mellitus of plane tree (Platanus orientalis L.) extracts and their antioxidant, antimicrobial and anticancer activities

https://doi.org/10.14715/cmb/2018.64.11.3

Esra Ucar

Medicinal and Aromatic Plant Department, Vocational School, Cumhuriyet University, 58140, Sivas, Turkey

Nuraniye Eruygur

Pharmacognosy Department, Faculty of Pharmacy, Selcuk University, 42250, Konya, Turkey

Mehmet Atas

Pharmaceutical Microbiology Department, Faculty of Pharmacy, Cumhuriyet University, 58140, Sivas, Turkey

Merve Ergul

Pharmacology Department, Faculty of Pharmacy, Cumhuriyet University, 58140, Sivas, Turkey

Mustafa Ergul

Biochemistry Department, Faculty of Pharmacy, Cumhuriyet University, 58140, Sivas, Turkey

Fazli Sozmen

Nanotechnology Engineering Department, Faculty of Engineering, Cumhuriyet University, 58140, Sivas, Turkey

Corresponding Author(s) : Esra Ucar

eucar@cumhuriyet.edu.tr

Cellular and Molecular Biology, Vol. 64 No. 11: Issue 11
Article Published : August 30, 2018

Abstract

Plane tree (Platanus orientalis L.) leaves have been employed for centuries in various countries due to their pharmacological value. Therefore, determination of the biological activity of the leaves is of interest. The aim of the study was to evaluate the inhibitory effects against Alzheimer's disease-related enzymes Acetylcholinesterase (AChE) and Butyrylcholinesterase (BuChE), diabetes mellitus related enzymes Î±-glucosidase and Î±-amylase. The antioxidant, anticancer, and antimicrobial activities of the leaves were also studied. According to the results, both water and methanol extracts of P. orientalis demonstrated more Î±-glucosidase and Î±-amylase inhibition activity than the antidiabetic drug-acarbose at the same concentration level. In addition, extracts showed good inhibition activity against AChE and BuChE. Significant results were obtained regarding antioxidant, anticancer, and antimicrobial activities. These results are very promising especially for the improvement of pharmaceutical formulations to treat various diseases such as age-related diseases, cancer, diabetes etc. and it is necessary to conduct further experiments.

Keywords

Plane tree Alzheimer's Disease Diabetes Antioxidant Activity Cytotoxic and Antimicrobial activities.

Ucar, E., Eruygur, N., Atas, M., Ergul, M., Ergul, M., & Sozmen, F. (2018). Determination of inhibitory activities of enzymes, related to Alzheimer’s disease and diabetes mellitus of plane tree (Platanus orientalis L.) extracts and their antioxidant, antimicrobial and anticancer activities. Cellular and Molecular Biology, 64(11), 13–19. https://doi.org/10.14715/cmb/2018.64.11.3

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References

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References

Zencirkiran M, Erken K. The Effect of Different Times Collecting Cutting and Auxin Treatments of The Rooting in Platanusorientalisl. (Oriental plane tree - cinar). J Anim Plant Sci 2012; 22:764–767.

Pourkhabbaz A, Rastin N, Olbrich A, Langenfeld HR, Polle A. Influence of environmental pollution on leaf properties of urban plane trees, Platanusorientalis L. Bull Environ Contam Toxicol 2010; 85: 251–255.

Hajhashemi V, Ghannadi A, Mousavi S. Antinociceptive study of extracts of Platanusorientalis leaves in mice. Res Pharm Sci 2011; 2:123–128.

Mitrokotsa D, Mitaku S, Demetzos C, Harvala C, Mentis A, Perez S, Kokkinopoulos D. Bioactive compounds from the buds of Platanusorientalis and isolation of a new kaempferol glycoside. Planta Med 1993; 59:517–520.

Mitrocotsa D, Bosch S, Mitaku S, Dimas C, Skaltsounis AL, Harvala C, Briand G, Roussakis C. Cytotoxicity against human leukemic cell lines and the activity on the expression of resistance genes of flavonoids from Platanusorientalis. Anticaner Res 1999; 19:2085–2088.

Ibrahim MA, Mansoor AA, Gross A, Ashfaq MK, Jacob M, Khan SI, Hamann MT. Methicillin-resistant Staphylococcus aureus (MRSA)-active metabolites from Platanusoccidentalius (American sycamore). J Nat Prod. 2009; 72:2141–2144.

Zargari A. Medicinal plants, 6th ed., Tehran University Publications, Tehran, 1990, pp. 469–470.

Pohanka M. Cholinesterases, A Target Of Pharmacology And Toxicology. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 2011; 155:219–230.

Giacobini E. Cholinesterases: New Roles in Brain Function and in Alzheimer's Disease. Neurochem Res 2003; 28:515-22.

Ali H, Houghtona PJ, Soumyanathb A. Î±-Amylase inhibitory activity of some Malaysian plants used to treat diabetes; with particular reference to Phyllanthusamarus. J Ethnopharmacology 2006; 3:449-455.

Bhandari MR, Anurakkun NJ, Hong G, Kawabata J. Î±-Glucosidase and Î±-amylase inhibitory activities of Nepalese medicinal herb Pakhanbhed (Bergeniaciliata Haw.). Food Chem 2008; 1:247-252.

Jian B, Zhang H, Han C, Liu J. Anti-Cancer Activities of Diterpenoids Derived from Euphorbia fischeriana Steud. Molecules 2018; 23:1-11.

Grollino MG, Raschellí G, Cordelli E, Villani P, Pieraccioli M, Paximadas I, Malandrino S, Bonassi S, Pacchierotti F. Cytotoxicity, genotoxicity and gene expression changes elicited by exposure of human hepatic cells to Ginkgo biloba leaf extract. Food Chem Toxicol 2017; 109:486-496.

Sacchetti G, Maietti S, Muzzoli M, Scaglianti M, Manfredini S, Radice M, Bruni R. Comparative evaluation of 11 essential oils of different origin as functional antioxidants, antiradicals and antimicrobials in foods. Food chemistry 2005; 91:621-632.

Clarke G, Ting KN, Wiart C, Fry J. High Correlation of 2,2-diphenyl-1-picrylhydrazyl (DPPH) Radical Scavenging, Ferric Reducing Activity Potential and Total Phenolics Content Indicates Redundancy in Use of All Three Assays to Screen for Antioxidant Activity of Extracts of Plants from the Malaysian Rainforest. Antioxidants 2013; 2:1-10.

Chun SS, Vattem DA, Lin YT, Shetty K. Phenolic antioxidants from clonal oregano (Origanumvulgare) with antimicrobial activity against Helicobacter pylori. Process Biochem 2005; 40:809-816.

Yang H, Dong Y, Du H, Shi H, Peng Y, Li X. Antioxidant Compounds from Propolis Collected in Anhui, China. Molecules 2011; 16:3444-3455.

Ellman GL, Courtney KD, Andres V, Featherstone RM. A New and Rapid Colorimetric Determination of Acetylcholinesterase Activity. Biochem Pharmacol 1961; 7:88–95.

Eloff JN, A sensitive and quick microplate method to determine the minimal inhibitory concentration of plant extracts for bacteria. Planta Med 1998; 64:711–713.

CLSI, Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically, Approved Standard, 9th ed., CLSI document M07-A9. Clinical and Laboratory Standards Institute, 950 West Valley Road, Suite 2500, Wayne, Pennsylvania 19087, USA, 2012.

CLSI, Reference Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts, Approved Standard, 2nd ed., NCCLS document M27- A2. CLSI, 940 West Valley Road, Suite 1400, Wayne, Pennsylvania 19087- 1898, USA, 2002.

Kuete V. Potential of Cameroonian plants and derived products against microbial infections: a review. Planta Med 2010; 76:1479–1491.

Awouafack MD, McGaw LJ, Gottfried S, Mbouangouere R, Tane P, Spiteller M, Eloff JN. Antimicrobial Activity and Cytotoxicity of the Ethanol Extract, Fractions and Eight Compounds Isolated from Eriosemarobustum (Faba-ceae). BMC Complement Altern Med 2013; 13:289-297.

Author biographies is not available.