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Vol. 65 No. 1: Issue 1

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Ameliorative effect of probiotics (Lactobacillus paracaseii and Protexin®) and prebiotics (propolis and bee pollen) on clindamycin and propionic acid-induced oxidative stress and altered gut microbiota in a rodent model of autism

https://doi.org/10.14715/cmb/2019.65.1.1
Kawther Aabed
Biology Department, College of Science, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia
Ramesa Shafi Bhat
Biochemistry Department, College of Sciences, King Saud University, Riyadh, Saudi Arabia
Nadine Moubayed
Botany and Microbiology, College of Sciences, King Saud University, Riyadh, Saudi Arabia
Manar Al-Mutiri
Central Laboratory, Female Center for Scientific and Medical Studies, King Saud University, Riyadh, Saudi Arabia
Majda Al-Marshoud
Central Laboratory, Female Center for Scientific and Medical Studies, King Saud University, Riyadh, Saudi Arabia
Alaa Al-Qahtani
Biology Department, College of Science, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia
Afaf Ansary
Central Laboratory, Female Center for Scientific and Medical Studies, King Saud University, Riyadh, Saudi Arabia

Corresponding Author(s) : Afaf Ansary

elansary@ksu.edu.sa

Cellular and Molecular Biology, Vol. 65 No. 1: Issue 1

Abstract

Colonization by toxin-producing bacteria in the gut plays a major role in bowel problems in autistic patients. Prebiotics can inhibit the growth of these pathogenic microbes by nourishing beneficial bacteria, while probiotics--live microorganisms--can balance the gut bacteria; thus, both together can maintain healthy bacteria in the gut. The present study was conducted to find the effect of probiotics and prebiotics in balancing the gut flora in a rodent model of autism linked with a clindamycin-induced altered gut. The effects of probiotics and prebiotics on oxidative stress markers in the brain were also evaluated. Eight groups of hamsters were assigned, with Group I serving as the control; Group II, as the autistic model, was treated with 250 mg propionic acid/kg BW/day for 3 days; Group III was treated with clindamycin 30 mg/kg BW for 3 days; Groups IV and V were treated with bee pollen and propolis (supposed prebiotics) at a dose of 250 mg/kg BW/day for 28 days; Group VI and Group VII were treated with Lactobacillus paracaseii and Protexin® (supposed probiotics) for 28 days; and finally, Group VIII was anorectally transplanted with stool from normal animals for 5 days. Remarkable changes were measured in oxidative stress markers, primarily glutathione and vitamin C, in the brains of hamsters in the propionic acid- and clindamycin-treated group. All probiotic/prebiotic treatments showed ameliorative effects; however, lactobacillus had the strongest effect. We conclude that pro-and prebiotic supplements may be effective to revive healthy digestive system function in autistic patients. The disappointing results of the fecal transplants suggest that further study is needed to develop an appropriate technique.

Keywords

Probiotic Prebiotic Autism Lactobacillus Fecal transplant Propolis Bee pollen Propionic acid Clindamycin.
Aabed, K., Shafi Bhat, R., Moubayed, N., Al-Mutiri, M., Al-Marshoud, M., Al-Qahtani, A., & Ansary, A. (2019). Ameliorative effect of probiotics (Lactobacillus paracaseii and Protexin®) and prebiotics (propolis and bee pollen) on clindamycin and propionic acid-induced oxidative stress and altered gut microbiota in a rodent model of autism. Cellular and Molecular Biology, 65(1), 1–7. https://doi.org/10.14715/cmb/2019.65.1.1
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