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Vol. 64 No. 12: Issue 12

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Hydrothermal synthesis of highly fluorescent and non-toxic carbon dots using Stevia rebaudiana Bertoni

https://doi.org/10.14715/cmb/2018.64.12.7
Fatemeh Ansari
Department of Nanobiotechnology, Razi University, Kermanshah, Iran
Danial Kahrizi
Department of Agronomy and Plant Breeding, Razi University, Kermanshah, Iran

Corresponding Author(s) : Danial Kahrizi

d.kahrizi@cellmolbiol.org

Cellular and Molecular Biology, Vol. 64 No. 12: Issue 12

Abstract

Herein, green, environmental-friendly and economical synthesize of luminescent carbon dots (CDs) was reported using the plant-based material, Stevia rebaudiana Bertoni, as a herbal bio-sweetener. In this regard, hydrothermal carbonization of Stevia was performed under mild conditions and without any chemical oxidizing agents. Exploring the morphological aspect, surface groups, chemical composition and structure of Stevia-based CDs have been carried out systematically using SEM-EDS, TEM, fluorescence, FT-IR, UV-Vis, and XRD techniques. UV–Vis spectra displayed signature absorption of CDs at 240 nm related to the π−π* transitions of C=C. Fluorescence spectra showed characteristic emission at the peak wavelength of 431 nm with a quantum yield of approximately 17.5%. Moreover, there was no living cell cytotoxicity for the as-prepared CDs confirmed by confocal microscopy. These results indicated that these plant-based CDs are ideal non-toxic promising markers for cellular bio-imaging.

Keywords

Carbon dot Stevia Hydrothermal Bio-imaging.
Ansari, F., & Kahrizi, D. (2018). Hydrothermal synthesis of highly fluorescent and non-toxic carbon dots using Stevia rebaudiana Bertoni. Cellular and Molecular Biology, 64(12), 32–36. https://doi.org/10.14715/cmb/2018.64.12.7
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