All-trans retinoic acid regulated prohibitin by retinoic acid receptor Î± in hypoxia-induced renal tubular epithelial cell injury

Tianbiao Zhou; Hong-Yan Li; Hongzhen Zhong; Zhiqing Zhong

doi:10.14715/cmb/2019.65.7.1

Issue

Vol. 65 No. 7: Issue 7

Issue Published : October 14, 2019

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All-trans retinoic acid regulated prohibitin by retinoic acid receptor Î± in hypoxia-induced renal tubular epithelial cell injury

https://doi.org/10.14715/cmb/2019.65.7.1

Tianbiao Zhou

Department of Nephrology, the Second Affiliated Hospital of Shantou University Medical College, 515041, Shantou, China

Hong-Yan Li

Department of Nephrology, Huadu District People's Hospital of Guangzhou, Southern Medical University, 510800, Guangzhou, China

Hongzhen Zhong

Department of Nephrology, the Second Affiliated Hospital of Shantou University Medical College, 515041, Shantou, China

Zhiqing Zhong

Department of Nephrology, the Second Affiliated Hospital of Shantou University Medical College, 515041, Shantou, China

Corresponding Author(s) : Hongzhen Zhong

hongzhenzhongstuc@126.com

Cellular and Molecular Biology, Vol. 65 No. 7: Issue 7
Article Published : September 30, 2019

Abstract

All-trans retinoic acid (ATRA) is a critical component in cell processes such as cell growth, differentiation and apoptosis, and it is also crucial in the regulation of extracellular matrix (ECM) deposition. Prohibitin (PHB) can regulate cell proliferation, apoptosis and differentiation. The current study investigated whether ATRA regulated PHB is induced by hypoxia/reoxygenation injury in renal tubular epithelial cells (RTEC), using gene interference treatments (knockdown or overexpression of RARÎ±). Our results indicate that ATRA can augment the expression of RARÎ± and PHB proteins and reduce the expression of TGF-Î²1, FN and Col-IV proteins. PHB expression was reduced in an ATRA treated RARÎ±- group, and TGF-Î²1, FN and Col-IV were up-regulated compared to the ATRA treated RARÎ±+ group. We postulate that ATRA can induce the PHB expression by RARÎ± in hypoxia/reperfusion related RTEC injury.

Keywords

All-trans retinoic acid (ATRA) RARÎ± Prohibitin Transforming growth factor beta 1 (TGF-Î²1) Renal tubular epithelial cells (RTEC) Oxidative stress Extracellular matrix (ECM).

Zhou, T., Li, H.-Y., Zhong, H., & Zhong, Z. (2019). All-trans retinoic acid regulated prohibitin by retinoic acid receptor Î± in hypoxia-induced renal tubular epithelial cell injury. Cellular and Molecular Biology, 65(7), 1–4. https://doi.org/10.14715/cmb/2019.65.7.1

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References

Hasegawa S, Nakano T, Torisu K, Tsuchimoto A, Eriguchi M, Haruyama N, Masutani K, Tsuruya K, Kitazono T. Vascular endothelial growth factor-C ameliorates renal interstitial fibrosis through lymphangiogenesis in mouse unilateral ureteral obstruction. Lab Invest 2017, 97(12):1439-1452.
Li X, Ma M, Zhang X, Deng L, Wang Y, Bian Z, Cai S, Peng B, Yang J, Chen Y. Ethanol extract of gardenia fruit alleviates renal interstitial fibrosis induced by unilateral ureteral obstruction in rats. Exp Ther Med 2017, 14(2):1381-1388.
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Tang PM, Zhang YY, Mak TS, Tang PC, Huang XR, Lan HY. Transforming growth factor-beta signalling in renal fibrosis: from Smads to non-coding RNAs. J Physiol 2018.
Liu ZM, Wang KP, Ma J, Guo Zheng S. The role of all-trans retinoic acid in the biology of Foxp3+ regulatory T cells. Cell Mol Immunol 2015, 12(5):553-557.
Siddikuzzaman, Guruvayoorappan C, Berlin Grace VM. All trans retinoic acid and cancer. Immunopharmacol Immunotoxicol 2011, 33(2):241-249.
Zhou TB, Drummen GP, Qin YH. The controversial role of retinoic acid in fibrotic diseases: analysis of involved signaling pathways. Int J Mol Sci 2012, 14(1):226-243.
Wang Q, Leader A, Tsang BK. Follicular stage-dependent regulation of apoptosis and steroidogenesis by prohibitin in rat granulosa cells. J Ovarian Res 2013, 6(1):23.
Anderson CJ, Kahl A, Qian L, Stepanova A, Starkov A, Manfredi G, Iadecola C, Zhou P. Prohibitin is a positive modulator of mitochondrial function in PC12 cells under oxidative stress. J Neurochem 2018.
Zhou TB, Qin YH, Li ZY, Xu HL, Zhao YJ, Lei FY. All-trans retinoic acid treatment is associated with prohibitin expression in renal interstitial fibrosis rats. Int J Mol Sci 2012, 13(3):2769-2782.
Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 2001, 25(4):402-408.
Bommareddy A, Crisamore K, Fillman S, Brozena S, Steigerwalt J, Landis T, Vanwert AL, Dwivedi C. Survivin Down-regulation by alpha-Santalol Is Not Mediated Through PI3K-AKT Pathway in Human Breast Cancer Cells. Anticancer Res 2015, 35(10):5353-5357.
Zhou TB, Qin YH, Lei FY, Huang WF, Drummen GP. Prohibitin is associated with antioxidative protection in hypoxia/reoxygenation-induced renal tubular epithelial cell injury. Sci Rep 2013, 3:3123.
Zhou TB, Ou C, Rong L, Drummen GP. Effect of all-trans retinoic acid treatment on prohibitin and renin-angiotensin-aldosterone system expression in hypoxia-induced renal tubular epithelial cell injury. J Renin Angiotensin Aldosterone Syst 2014, 15(3):243-249.
Zhong C, Pu L, Fang M, Rao J, Wang X. ATRA Regulates Innate Immunity in Liver Ischemia/Reperfusion Injury via RARalpha/Akt/Foxo1 Signaling. Biol Pharm Bull 2018, 41(4):530-535.
Kalitin NN, Karamysheva AF. RARalpha mediates all-trans-retinoic acid-induced VEGF-C, VEGF-D, and VEGFR3 expression in lung cancer cells. Cell Biol Int 2016, 40(4):456-464.
Zhang X, Yu Q, Jiang W, Bi Y, Zhang Y, Gong M, Wei X, Li T, Chen J. All-trans retinoic acid suppresses apoptosis in PC12 cells injured by oxygen and glucose deprivation via the retinoic acid receptor alpha signaling pathway. Mol Med Rep 2014, 10(5):2549-2555.

References

Hasegawa S, Nakano T, Torisu K, Tsuchimoto A, Eriguchi M, Haruyama N, Masutani K, Tsuruya K, Kitazono T. Vascular endothelial growth factor-C ameliorates renal interstitial fibrosis through lymphangiogenesis in mouse unilateral ureteral obstruction. Lab Invest 2017, 97(12):1439-1452.

Li X, Ma M, Zhang X, Deng L, Wang Y, Bian Z, Cai S, Peng B, Yang J, Chen Y. Ethanol extract of gardenia fruit alleviates renal interstitial fibrosis induced by unilateral ureteral obstruction in rats. Exp Ther Med 2017, 14(2):1381-1388.

Xiao Y, Liu J, Peng Y, Xiong X, Huang L, Yang H, Zhang J, Tao L. GSTA3 Attenuates Renal Interstitial Fibrosis by Inhibiting TGF-Beta-Induced Tubular Epithelial-Mesenchymal Transition and Fibronectin Expression. PLoS One 2016, 11(9):e0160855.

Tang PM, Zhang YY, Mak TS, Tang PC, Huang XR, Lan HY. Transforming growth factor-beta signalling in renal fibrosis: from Smads to non-coding RNAs. J Physiol 2018.

Liu ZM, Wang KP, Ma J, Guo Zheng S. The role of all-trans retinoic acid in the biology of Foxp3+ regulatory T cells. Cell Mol Immunol 2015, 12(5):553-557.

Siddikuzzaman, Guruvayoorappan C, Berlin Grace VM. All trans retinoic acid and cancer. Immunopharmacol Immunotoxicol 2011, 33(2):241-249.

Zhou TB, Drummen GP, Qin YH. The controversial role of retinoic acid in fibrotic diseases: analysis of involved signaling pathways. Int J Mol Sci 2012, 14(1):226-243.

Wang Q, Leader A, Tsang BK. Follicular stage-dependent regulation of apoptosis and steroidogenesis by prohibitin in rat granulosa cells. J Ovarian Res 2013, 6(1):23.

Anderson CJ, Kahl A, Qian L, Stepanova A, Starkov A, Manfredi G, Iadecola C, Zhou P. Prohibitin is a positive modulator of mitochondrial function in PC12 cells under oxidative stress. J Neurochem 2018.

Zhou TB, Qin YH, Li ZY, Xu HL, Zhao YJ, Lei FY. All-trans retinoic acid treatment is associated with prohibitin expression in renal interstitial fibrosis rats. Int J Mol Sci 2012, 13(3):2769-2782.

Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 2001, 25(4):402-408.

Bommareddy A, Crisamore K, Fillman S, Brozena S, Steigerwalt J, Landis T, Vanwert AL, Dwivedi C. Survivin Down-regulation by alpha-Santalol Is Not Mediated Through PI3K-AKT Pathway in Human Breast Cancer Cells. Anticancer Res 2015, 35(10):5353-5357.

Zhou TB, Qin YH, Lei FY, Huang WF, Drummen GP. Prohibitin is associated with antioxidative protection in hypoxia/reoxygenation-induced renal tubular epithelial cell injury. Sci Rep 2013, 3:3123.

Zhou TB, Ou C, Rong L, Drummen GP. Effect of all-trans retinoic acid treatment on prohibitin and renin-angiotensin-aldosterone system expression in hypoxia-induced renal tubular epithelial cell injury. J Renin Angiotensin Aldosterone Syst 2014, 15(3):243-249.

Zhong C, Pu L, Fang M, Rao J, Wang X. ATRA Regulates Innate Immunity in Liver Ischemia/Reperfusion Injury via RARalpha/Akt/Foxo1 Signaling. Biol Pharm Bull 2018, 41(4):530-535.

Kalitin NN, Karamysheva AF. RARalpha mediates all-trans-retinoic acid-induced VEGF-C, VEGF-D, and VEGFR3 expression in lung cancer cells. Cell Biol Int 2016, 40(4):456-464.

Zhang X, Yu Q, Jiang W, Bi Y, Zhang Y, Gong M, Wei X, Li T, Chen J. All-trans retinoic acid suppresses apoptosis in PC12 cells injured by oxygen and glucose deprivation via the retinoic acid receptor alpha signaling pathway. Mol Med Rep 2014, 10(5):2549-2555.

Author biographies is not available.