Issue

Vol. 66 No. 7: Issue 7

The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.

CircB3GNTL1 and miR-598 regulation effects on proliferation, apoptosis, and glutaminolysis in gastric cancer cells

https://doi.org/10.14715/cmb/2020.66.7.4
Bin Ye
Department of Gastroenterology, Shengzhou People's Hospital (the First Affiliated Hospital of University Shengzhou Branch), Shengzhou, 312400, China
Shan Yu
Department of Gastroenterology, Shengzhou People's Hospital (the First Affiliated Hospital of University Shengzhou Branch), Shengzhou, 312400, China
Jingang Wang
Department of Gastroenterology, Shengzhou People's Hospital (the First Affiliated Hospital of University Shengzhou Branch), Shengzhou, 312400, China
Yuhan Ren
Department of Gastroenterology, Shengzhou People's Hospital (the First Affiliated Hospital of University Shengzhou Branch), Shengzhou, 312400, China

Corresponding Author(s) : Yuhan Ren

yuhanren0207@outlook.com

Cellular and Molecular Biology, Vol. 66 No. 7: Issue 7

Abstract

This study was performed to research circB3GNTL1 control, miR-598 and its mechanism for cell proliferation, apoptosis and glutamine breakdown. For this purpose, CirB3GNTL1 and miR-598 expressions were detected by qRT-PCR in gastric and cell lines; MTT tests were performed to detect proliferation; flow cytometry was established in flow; glutamine decomposition was evaluated with glutamine, glutamic acid and α-keto-glutaric acid (α-KG) expression; Bcl-2, PCNA, ASCT2 and GLS1 expression levels were calculated; Methods of expression were calculated. The results showed that CircB3GNTL1 expression was up-regulated and miR-598 expression was up-regulated in gastric cancer tissues and cell lines; Knockdown circB3GNTL1 prevented proliferation and glutamine decomposition of the gastric cancer cells and induced apoptosis compared with normal para-cancers and gastric cancer cell lines. Results circB3GNTL1 can target and control miR-598 expression, and miR-598 can reverse the proliferation, apoptosis, and decomposition of glutamine from gastric cancer cells by knockdown circB3GNTL1. It was concluded that CirB3GNTL1 prevents decomposition of glutamines and induces apoptosis by controlling miR-598 in gastric cancer cells.

Keywords

circB3GNTL1 miR-598 gastric cancer proliferation apoptosis glutaminolysis
Ye, B., Yu, S., Wang, J., & Ren, Y. (2020). CircB3GNTL1 and miR-598 regulation effects on proliferation, apoptosis, and glutaminolysis in gastric cancer cells. Cellular and Molecular Biology, 66(7), 18–23. https://doi.org/10.14715/cmb/2020.66.7.4
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. Guggenheim DE, Shah MA. Gastric cancer epidemiology and risk factors. J Surg Oncol 2013; 107(3): 230-6.
  2. Karimi P, Islami F, Anandasabapathy S, Freedman ND, Kamangar F. Gastric cancer: descriptive epidemiology, risk factors, screening, and prevention. Cancer Epidemiol Biomarkers Prev 2014; 23(5): 700-13.
  3. Ferlay J, Soerjomataram I, Dikshit R, et al. Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer, 2015; 136(5): 359-386.
  4. Li X, Liu S, Yan J, Peng L, Chen M, Yang J, Zhang G. The Characteristics, Prognosis, and Risk Factors of Lymph Node Metastasis in Early Gastric Cancer. Gastroenterol Res Pract. 2018, 2018:6945743.
  5. Wilusz JE, Sharp PA. A circuitous route to noncoding RNA. Science, 2013，340 (6131): 440–1.
  6. Sanger HL, Klotz G, Riesner D, et al. Viroids are single-stranded covalently closed circular RNA molecules existing as highly base-paired rod-like structures. Proc Natl Acad Sci USA, 1976; 73(11): 3852-3856.
  7. Li XW, Yang WH, Xu J. Circular RNA in gastric cancer. Chin Med J (Engl), 2020; 133(15): 1868-1877.
  8. Li X, Lin SH, Mo ZF, et al. CircRNA_100395 inhibits cell proliferation and metastasis in ovarian cancer via regulating miR-1228/p53/epithelial-mesenchymal transition (EMT) axis. J Cancer. 2020, 11(3): 599-609.
  9. Yang BM, Zhao JR, Huo TT, et al. Effects of CircRNA-ITCH on proliferation and apoptosis of hepatocellular carcinoma cells through inhibiting Wnt/β-catenin signaling pathway. J BUON, 2020; 25(3): 1368-1374.
  10. Hang Y, Liu H, Li WD, et al. CircRNA_100269 is downregulated in gastric cancer and suppresses tumor cell growth by targeting miR-630. Aging (Albany NY), 2017, 9(6): 1585-1594.
  11. Ding LX, Zhao YY, Dang SW, et al. Circular RNA circ-DONSON facilitates gastric cancer growth and invasion via NURF complex dependent activation of transcription factor SOX4. Mol Cancer, 2019; 18(1): 45.
  12. Zhang J, Hou LD, Liang R, et al. CircDLST promotes the tumorigenesis and metastasis of gastric cancer by sponging miR-502-5p and activating the NRAS/MEK1/ERK1/2 signaling. Mol Cancer 2019; 18(1): 80.
  13. Shin VY, Chu KM. MiRNA as potential biomarkers and therapeutic targets for gastric cancer. World J Gastroenterol. 2014, 20(30):10432-9.
  14. Zhang XY, Zhang PY. Gastric cancer: somatic genetics as a guide to therapy. J Med Genet. 2017; 54(5):305-312.
  15. Li R, Jiang J, Shi H, Qian H, Zhang X, Xu W. CircRNA: a rising star in gastric cancer. Cell Mol Life Sci 2020; 77(9):1661-1680.
  16. Li XW, Yang WH, Xu J. Circular RNA in gastric cancer. Chin Med J (Engl), 2020, 133(15): 1868-1877.
  17. Lai ZY, Yang Y, Yan YC, et al. Analysis of co-expression networks for circular RNAs and mRNAs reveals that circular RNAs hsa_circ_0047905, hsa_circ_0138960 and has-circRNA7690-15 are candidate oncogenes in gastric cancer. Cell Cycle, 2017; 16(23): 2301-2311.
  18. Zhang J, Liu H, Hou LD, et al. Circular RNA_LARP4 inhibits cell proliferation and invasion of gastric cancer by sponging miR-424-5p and regulating LATS1 expression. Mol Cancer, 2017; 16(1): 151.
  19. Li QC, Tian Y, Liang Y, et al. CircHIPK3/miR-876-5p/PIK3R1 axis regulates regulation proliferation, migration, invasion, and glutaminolysis in gastric cancer cells. Cancer Cell Int, 2020; 391(20).
  20. Liu M, Liu KD, Zhang L, Cai J, Yao HW, Bai YK, Zhang ZT. Circ_0009910 regulates growth and metastasis and is associated with poor prognosis in gastric cancer. Eur Rev Med Pharmacol Sci. 2018; 22(23):8248-8256.
  21. Jin L, Alesi GN, Kang S. Glutaminolysis as a target for cancer therapy. Oncogene, 2015; 35(28): 3619-3625.
  22. Van Geldermalsen M, Wang Q, Nagarajah R, et al. ASCT2/SLC1A5 controls glutamine uptake and tumour growth in triple-negative basal-like breast cancer. Oncogene, 2016; 35(24): 3201-3208.
  23. Pang L, Wang J, Fan Y, Xu R, Bai Y, Bai L. Correlations of TNM staging and lymph node metastasis of gastric cancer with MRI features and VEGF expression. Cancer Biomark. 2018; 23(1):53-59.
  24. Liu N, Yang H, Wang H. miR-598 acts as a tumor suppressor in human gastric cancer by targeting IGF-1R. OncoTargets and therapy, 2018; (11): 2911-2913.
  25. Zeng XY, Yuan J, Wang C, et al. circCELSR1 facilitates ovarian cancer proliferation and metastasis by sponging miR-598 to activate BRD4 signals. Mol Med 2020; 26(1): 70.
Read More
Author biographies is not available.
Crossref
Scopus
Google Scholar
Europe PMC
Download this PDF file
PDF
Statistic
Read Counter : 902 Download : 512