Ent-Dihydrotucumanoic acid promotes apoptosis in PC-3 human prostate cancer cells

Min Zhou; Weibing Li

doi:10.14715/cmb/2019.65.3.17

Issue

Vol. 65 No. 3: Issue 3

Issue Published : March 31, 2019

The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.

Ent-Dihydrotucumanoic acid promotes apoptosis in PC-3 human prostate cancer cells

https://doi.org/10.14715/cmb/2019.65.3.17

Min Zhou

Department of Traditional Chinese Medicine, Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research & The Affiliated Cancer Hospital of Nanjing Medical University, 42, Baiziting, Xuanwu Distinct, 210009 Nanjing, Jiangsu Province, China

Weibing Li

Corresponding Author(s) : Min Zhou

av99175@163.com

Cellular and Molecular Biology, Vol. 65 No. 3: Issue 3
Article Published : March 31, 2019

Abstract

Prostate cancer (PC) has become a disease that pose a serious threat to men's health and life. In recent years, due to the changes of environment, lifestyle and other factors, the incidence of PC has been increasing rapidly in recent years, which is a serious threat to men's health. Ent-Dihydrotucumanoic Acid (DTA) is a compound isolated from Asteraceae of gymnosperms, which has many pharmacological effects. The effect of DTA on the growth of tumor cell line was studied by CCK-8 method, mitochondrial membrane potential and apoptosis were detected by flow cytometry, apoptosis-related genes were detected by Western blot assay, and the absorptivity of Caspase-3 and Caspase-9 was measured by spectrophotometer. It was found that DTA induces apoptosis of human prostate cancer cell line PC3 through mitochondrial pathway, thus preventing the development of prostate cancer. It lays the experimental foundation for the further development of DTA.

Keywords

Ent-Dihydrotucumanoic Acid PC-3 Apoptosis Prostate cancer.

Zhou, M., & Li, W. (2019). Ent-Dihydrotucumanoic acid promotes apoptosis in PC-3 human prostate cancer cells. Cellular and Molecular Biology, 65(3), 114–118. https://doi.org/10.14715/cmb/2019.65.3.17

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References

Baade PD, Youlden DR, Krnjacki LJ. International epidemiology of prostate cancer: Geographical distribution and secular trends. Mol Nutr Food Res 2010; 53: 171-184.
Siegel RL, Miller KD, Jemal A. Cancer statistics, 2018. CA A Cancer J Clin 2018; 60: 277-300.
Jing S, Yan Z, Yan L, Cheng-Quan Y, Xian-Kui L, Ying Z. Systematic Analysis on the GSTM1 Null Phenotype and Prostate Cancer Risk in Chinese People. Asian Pac J Cancer Prev 2015; 16: 2009.
Fuessel S, Schmitz M, Richter K, Meye A, Zastrow S, Linné C, et al. VACCINATION OF HORMONE-REFRACTORY PROSTATE CANCER PATIENTS WITH PEPTIDE COCKTAIL-LOADED DENDRITIC CELLS: IMMUNOLOGICAL RESULTS OF A PHASE 1 CLINICAL TRIAL. Eur Urol Supplements 2006; 5:162.
Yip K, Alonzi R. Carbogen gas and radiotherapy outcomes in prostate cancer. Ther Adv Urol 2013; 5: 25-34.
Grayer RJ, Kokubun T. Plant–fungal interactions: the search for phytoalexins and other antifungal compounds from higher plants. Phytochemistry 2001; 56: 253-263.
Hui HY, Wu N, Wu M, Liu Y, Xiao SX, Zhang MF. Dihydroartemisinin suppresses growth of squamous cell carcinoma A431 cells by targeting the Wnt/Î²-catenin pathway. Anticancer Drugs 2015; 27: 99-105.
Sultana S, Asif HM, Nazar HM, Akhtar N, Rehman JU, Rehman RU. Medicinal plants combating against cancer--a green anticancer approach. Asian Pac J Cancer Prev 2014; 15: 4385-4394.
Wang CY, Bai XY, Wang CH. Traditional Chinese Medicine: A Treasured Natural Resource of Anticancer Drug Research and Development. Am J Chin Med 2014; 42: 543-559.
Gonzalez-Chavez MM, Arana-Argaez V, Zapata-Morales JR, Avila-Venegas AK, Alonso-Castro AJ, Isiordia-Espinoza M, et al. Pharmacological evaluation of 2-angeloyl ent-dihydrotucumanoic acid. Pharm Biol 2017; 55: 873-879.
Alonso-Castro AJ, Gonzalez-Chavez MM, Zapata-Morales JR, Verdinez-Portales AK, Sanchez-Recillas A, Ortiz-Andrade R, et al. Antinociceptive Activity of Ent-Dihydrotucumanoic Acid Isolated from Gymnosperma glutinosum Spreng Less. Drug Dev Res 2017; 78: 340-348.
Gomez-Flores R, Espinosa-Ramos D, Quintanilla-Licea R, Barrón-Gonzalez MP, Tamez-Guerra P, Tamez-Guerra R, et al. ANTIMICROBIAL ACTIVITY OF GYMNOSPERMA GLUTINOSUM (SPRENG.) LESS. (ASTERACEAE) METHANOL EXTRACTS AGAINST HELICOBACTER PYLORI. Afr J Tradit Complement Altern Med 2016; 13: 55-59.
Alonso-Castro AJ, González-Chávez MM, Zapata-Morales JR, Verdinez-Portales AK, Sánchez-Recillas A, Ortiz-Andrade R, et al. Antinociceptive Activity of Ent-Dihydrotucumanoic Acid Isolated from Gymnosperma glutinosum Spreng Less. Drug Dev Res 2017; 78: 340-348.
Heidenreich A, Bellmunt J, Bolla M, Joniau S, Mason M, Matveev V, et al. EAU guidelines on prostate cancer. Part 1: screening, diagnosis, and treatment of clinically localised disease. Eur Urol 2011; 59: 61-71.
Harrington KJ, Spitzweg C, Bateman AR, Morris JC, Vile RG. GENE THERAPY FOR PROSTATE CANCER: CURRENT STATUS AND FUTURE PROSPECTS. J Urol 2018; 166: 1220-1233.
Bommareddy A, Eggleston W, Prelewicz S, Antal A, Witczak Z, Mccune DF, et al. Chemoprevention of prostate cancer by major dietary phytochemicals. Anticancer Res 2013; 33: 4163-4174.
Shi Z, Wu D, Tang R, Li X, Chen R, Xue S, et al. Silencing of HMGA2 promotes apoptosis and inhibits migration and invasion of prostate cancer cells. J Biosci 2016; 41: 229-236.
Norberg E, Orrenius S, Zhivotovsky B. Mitochondrial regulation of cell death: Processing of apoptosis-inducing factor (AIF). Biochem Biophys Res Commun 2010; 396: 95-100.
Caterina I, Chakraborty AR, Alina N, Bahr JC, Zhirong Z, Stefania P, et al. Loss of the proteins Bak and Bax prevents apoptosis mediated by histone deacetylase inhibitors. J Cell Cycle 2013; 12: 2829-2838.
Qian H, Jing P, Weiping L, Xiaoli H, Ling C, Xinlian C, et al. Elevated cleaved caspase-3 is associated with shortened overall survival in several cancer types. Int J Clin Exp Pathol 2014; 7: 5057-5070.
Korsmeyer SJ. Bcl-2 initiates a new category of oncogenes: regulators of cell death. J Blood 1992; 80: 879-886.
Korsmeyer SJ, Shutter JR, Veis DJ, Merry DE, Oltvai ZN. Bcl-2/Bax: a rheostat that regulates an anti-oxidant pathway and cell death. J Seminars Cancer Biol 1993; 4: 327.
Chipuk JE, Green D. Do inducers of apoptosis trigger caspase-independent cell death? Nat Rev Mol Cell Biol 2005; 6: 268-275.
Singh AV, Xiao D, Lew KL, Dhir R, Singh S. Sulforaphane induces caspase-mediated apoptosis in cultured PC-3 human prostate cancer cells and retards growth of PC-3 xenografts in vivo. Carcinogenesis 2004; 25: 83-90.
Armwood S, Juma BF, Ombito JO, Majinda RRT, Gwebu ET. Effect of erythrinaline alkaloids from Erythrina lysistemon on human recombinant caspase-3. Afr J Pharm Pharmacol 2018; 12: 183-187.

References

Baade PD, Youlden DR, Krnjacki LJ. International epidemiology of prostate cancer: Geographical distribution and secular trends. Mol Nutr Food Res 2010; 53: 171-184.

Siegel RL, Miller KD, Jemal A. Cancer statistics, 2018. CA A Cancer J Clin 2018; 60: 277-300.

Jing S, Yan Z, Yan L, Cheng-Quan Y, Xian-Kui L, Ying Z. Systematic Analysis on the GSTM1 Null Phenotype and Prostate Cancer Risk in Chinese People. Asian Pac J Cancer Prev 2015; 16: 2009.

Fuessel S, Schmitz M, Richter K, Meye A, Zastrow S, Linné C, et al. VACCINATION OF HORMONE-REFRACTORY PROSTATE CANCER PATIENTS WITH PEPTIDE COCKTAIL-LOADED DENDRITIC CELLS: IMMUNOLOGICAL RESULTS OF A PHASE 1 CLINICAL TRIAL. Eur Urol Supplements 2006; 5:162.

Yip K, Alonzi R. Carbogen gas and radiotherapy outcomes in prostate cancer. Ther Adv Urol 2013; 5: 25-34.

Grayer RJ, Kokubun T. Plant–fungal interactions: the search for phytoalexins and other antifungal compounds from higher plants. Phytochemistry 2001; 56: 253-263.

Hui HY, Wu N, Wu M, Liu Y, Xiao SX, Zhang MF. Dihydroartemisinin suppresses growth of squamous cell carcinoma A431 cells by targeting the Wnt/Î²-catenin pathway. Anticancer Drugs 2015; 27: 99-105.

Sultana S, Asif HM, Nazar HM, Akhtar N, Rehman JU, Rehman RU. Medicinal plants combating against cancer--a green anticancer approach. Asian Pac J Cancer Prev 2014; 15: 4385-4394.

Wang CY, Bai XY, Wang CH. Traditional Chinese Medicine: A Treasured Natural Resource of Anticancer Drug Research and Development. Am J Chin Med 2014; 42: 543-559.

Gonzalez-Chavez MM, Arana-Argaez V, Zapata-Morales JR, Avila-Venegas AK, Alonso-Castro AJ, Isiordia-Espinoza M, et al. Pharmacological evaluation of 2-angeloyl ent-dihydrotucumanoic acid. Pharm Biol 2017; 55: 873-879.

Alonso-Castro AJ, Gonzalez-Chavez MM, Zapata-Morales JR, Verdinez-Portales AK, Sanchez-Recillas A, Ortiz-Andrade R, et al. Antinociceptive Activity of Ent-Dihydrotucumanoic Acid Isolated from Gymnosperma glutinosum Spreng Less. Drug Dev Res 2017; 78: 340-348.

Gomez-Flores R, Espinosa-Ramos D, Quintanilla-Licea R, Barrón-Gonzalez MP, Tamez-Guerra P, Tamez-Guerra R, et al. ANTIMICROBIAL ACTIVITY OF GYMNOSPERMA GLUTINOSUM (SPRENG.) LESS. (ASTERACEAE) METHANOL EXTRACTS AGAINST HELICOBACTER PYLORI. Afr J Tradit Complement Altern Med 2016; 13: 55-59.

Alonso-Castro AJ, González-Chávez MM, Zapata-Morales JR, Verdinez-Portales AK, Sánchez-Recillas A, Ortiz-Andrade R, et al. Antinociceptive Activity of Ent-Dihydrotucumanoic Acid Isolated from Gymnosperma glutinosum Spreng Less. Drug Dev Res 2017; 78: 340-348.

Heidenreich A, Bellmunt J, Bolla M, Joniau S, Mason M, Matveev V, et al. EAU guidelines on prostate cancer. Part 1: screening, diagnosis, and treatment of clinically localised disease. Eur Urol 2011; 59: 61-71.

Harrington KJ, Spitzweg C, Bateman AR, Morris JC, Vile RG. GENE THERAPY FOR PROSTATE CANCER: CURRENT STATUS AND FUTURE PROSPECTS. J Urol 2018; 166: 1220-1233.

Bommareddy A, Eggleston W, Prelewicz S, Antal A, Witczak Z, Mccune DF, et al. Chemoprevention of prostate cancer by major dietary phytochemicals. Anticancer Res 2013; 33: 4163-4174.

Shi Z, Wu D, Tang R, Li X, Chen R, Xue S, et al. Silencing of HMGA2 promotes apoptosis and inhibits migration and invasion of prostate cancer cells. J Biosci 2016; 41: 229-236.

Norberg E, Orrenius S, Zhivotovsky B. Mitochondrial regulation of cell death: Processing of apoptosis-inducing factor (AIF). Biochem Biophys Res Commun 2010; 396: 95-100.

Caterina I, Chakraborty AR, Alina N, Bahr JC, Zhirong Z, Stefania P, et al. Loss of the proteins Bak and Bax prevents apoptosis mediated by histone deacetylase inhibitors. J Cell Cycle 2013; 12: 2829-2838.

Qian H, Jing P, Weiping L, Xiaoli H, Ling C, Xinlian C, et al. Elevated cleaved caspase-3 is associated with shortened overall survival in several cancer types. Int J Clin Exp Pathol 2014; 7: 5057-5070.

Korsmeyer SJ. Bcl-2 initiates a new category of oncogenes: regulators of cell death. J Blood 1992; 80: 879-886.

Korsmeyer SJ, Shutter JR, Veis DJ, Merry DE, Oltvai ZN. Bcl-2/Bax: a rheostat that regulates an anti-oxidant pathway and cell death. J Seminars Cancer Biol 1993; 4: 327.

Chipuk JE, Green D. Do inducers of apoptosis trigger caspase-independent cell death? Nat Rev Mol Cell Biol 2005; 6: 268-275.

Singh AV, Xiao D, Lew KL, Dhir R, Singh S. Sulforaphane induces caspase-mediated apoptosis in cultured PC-3 human prostate cancer cells and retards growth of PC-3 xenografts in vivo. Carcinogenesis 2004; 25: 83-90.

Armwood S, Juma BF, Ombito JO, Majinda RRT, Gwebu ET. Effect of erythrinaline alkaloids from Erythrina lysistemon on human recombinant caspase-3. Afr J Pharm Pharmacol 2018; 12: 183-187.

Author biographies is not available.