Issue

Vol. 65 No. 3: Issue 3

The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.

Effects of methyl jasmonate and phloroglucinol on thebaine and sanguinarine production in cell suspension culture of Persian poppy (Papaver bracteatum Lindl.)

https://doi.org/10.14715/cmb/2019.65.3.2
Taraneh Dastmalchi
Department of Plant Breeding and Biotechnology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Mansour Omidi
College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
Reza Azizinezhad
Department of Plant Breeding and Biotechnology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Shamsali Rezazadeh
Department of Pharmacognosy, Institute of Medicinal Plants, ACECR, Karaj, Iran
Alireza Etminan
Department of Plant Breeding, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran

Corresponding Author(s) : Mansour Omidi

momidi@ut.ac.ir

Cellular and Molecular Biology, Vol. 65 No. 3: Issue 3

Abstract

The biosynthesis path engineering could be very promising for mass production of alkaloids by applying elicitors in the cell suspension culture of Persian poppy (Papaver bracteatum Lindl.). In this work, the effects of different concentrations of methyl jasmonate (MJ) and phloroglucinol (PG) on thebaine and sanguinarine productions in vitro were investigated. Roots as explant and supplementing 3 mg L–1 2,4-Dichlorophenoxyacetic acid with 0.5 mg L–1 Benzyl amino purine to modified MS medium were selected to achieve the most efficient combination for callus induction and production of callus fresh and dry weights. At 48 h after treatment, the addition of PG and MJ individually and in combination together significantly increased both thebaine and sanguinarine contents than the control. The results of high-performance liquid chromatography (HPLC) detection indicated that the highest production rate has been achieved through a synergic effect of two elicitors after 48 h. Results revealed that adding 200 μM of MJ and 100 mg L–1 PG increased thebaine and sanguinarine contents by 56.36 and 107.71–fold than control cells, respectively.

Keywords

Cell suspension culture Methyljasmonate Phloroglucinol Sanguinarine Thebaine Papaver bracteatum Lindl.
Dastmalchi, T., Omidi, M., Azizinezhad, R., Rezazadeh, S., & Etminan, A. (2019). Effects of methyl jasmonate and phloroglucinol on thebaine and sanguinarine production in cell suspension culture of Persian poppy (Papaver bracteatum Lindl.). Cellular and Molecular Biology, 65(3), 11–17. https://doi.org/10.14715/cmb/2019.65.3.2
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. Sharghi N, Lalezari I. Papaver bracteatum Lindl., a highly rich source of thebaine. Nature 1967; 213:1244.
  2. Carolan, J.C., Hook, I.L.I., Chase, M.W., Kadereit, J.W., Hodkinson, T.R. 2006. Phylogenetics of Papaver and related genera based on DNA sequences from IST nuclear tibosomal DANN and plastid trnL intron and trnL-F intergenic spacers. Ann Bot 2006; 98;141-155.
  3. Goldblatt P. Biosystematic studies in Papaver section Oxytona. Ann Mo Bot Gard 1974; 61:264–296.
  4. Schmeller T, Wink M. Utilization of Alkaloids in Modern Medicine, in: Roberts, M.F., Wink, M. (Eds.), Alkaloids- biochemistry, ecology and medicinal applications. Plenum Press., New York, 1998. pp. 435-459.
  5. Park SU, Yu M, Facchini PJ. Modulation of berberine bridge enzyme levels in transgenic root cultures of California poppy alters the accumulation of benzophenanthridine alkaloids. Plant Mol Biol 203; 51:153-164.
  6. Trujillo-Villanueva K, Rubio-Pine J, Monforte-Gonzalez M, Ramirez- Benitez E, Vazquez-Flota F. The sequential exposure tojasmonate, salicylic acid and yeast extract promotes sanguinarine accumulationin argemone mexicana cell cultures. Biotechnol Lett 2012; 34:379–385.
  7. Tisserat B, Berhow M. Production of pharmaceuticals from Papaver cultivars. In vitroEng Life Sci 2009; 9:190-196.
  8. Kuete V. In: Toxicological Survey of African Medicinal Plants. Kuete V. (ed). Elsevier, Netherland, 2014, pp. 611-633.
  9. Smetanska I. In Food biotechnology: Production of secondary metabolites using plant cell cultures. Springer, Berlin, 2008.
  10. Murthy HN, Lee EJ, Paek KY. Production of secondary metabolites from cell and organ cultures: strategies and approaches for biomass improvement and metabolite accumulation. PCTOC J 2014; 118:1-16.
  11. Zhao J, Davis LC, Verpoorte R. Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnoladv. 2005; 23:283-333.
  12. Zare N, Farjaminezhad R, Asghari-Zakaria R, Farjaminezhad M. Enhanced thebaine production in Papaver bracteatum cell suspension culture by combination of elicitation and precursor feeding. Nat Prod Res 2014; 28:711-717.
  13. Balbi V, Devoto A. Jasmonate signalling network in Arabidopsis thaliana: crucial regulatory nodes and new physiological scenarios. New Phytol 2008; 177:301–318.
  14. Da Silva JAT, Dobranszki J, Ross S. Phloroglucinol in plant tissue culture. In Vitro Cell Dev Biol Plant 2003; 49:1-16.
  15. Berardi G, Infante R, Neri D. Micropropagation of Pyruscalleryana Dcn. from seedlings. Sci Hort 1993; 53:157–165.
  16. Manjula S, Anitha T, Daniel B, Nair GM. In vitro plantlet regeneration of Aristolochia indica through axillary shoot multiplication and organogenesis. Plant Cell Tissue Organ Cult 1997; 51:145–148.
  17. Hammatt N, Grant NJ. Micropropagation of mature British wild cherry. Pctoc J 1997; 47:103-110.
  18. Cho HY, Lee-Parsons CWT, Yoon SYH, Rhee HS, Park JM. Enhanced benzophenanthridine alkaloid production and protein expression with combined elicitor in Eschscholtziacalifornica suspension cultures. Biotechnol Lett 2007; 29:2001-2005.
  19. Wang J, Qian J, Yao L, Lu Y. Enhanced production of flavonoids by methyl jasmonate elicitation in cell suspension culture of Hypericum perforatum. BioB 2015; 2(1):5.
  20. Kumar P, Srivastava V, Chaturvedi R, Sundar D, BisariaVS. Elicitor enhanced production of protoberberine alkaloids from in vitro cell suspension cultures of Tinospora cordifolia (Willd.) Miers ex Hook. F. &Thoms. Pctoc J 2017; 130:417-426.
  21. Murashige T, Skoog F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 1962; 15:473-497.
  22. Cho HY, Son SY, Rhee HS, Yoon SYH, Lee-Parsons CW, Park JM. Synergistic effects of sequential treatment with methyl jasmonate, salicylic acid and yeast extract on benzophenanthridine alkaloid accumulation and protein expression in Eschscholtziacalifornica suspension cultures. J Biotechnol 2008; 135:117–122.
  23. Pedroso MC, Pais MS. Plant regeneration from embryogenic suspension cultures ofCamellia japonica. In vitro Cell Dev Biol Plant 1995; 31:31-35.
  24. Kim SW, In DS, Kim TJ, Liu JR. High frequency somatic embryogenesis and plant regeneration in petiole and leaf explant cultures and petiole-derived embryogenic cell suspension cultures of Hylomeconvernalis. Pctoc J 2003; 74:163-167.
  25. Yang JL, Zhao B, Seong ES, Kim MJ, Kang WH, Kim NY, Yu CY, Li CH. Callus induction and high-efficiency plant regeneration via somatic embryogenesis in Papaver nudicaule L., an ornamental medicinal plant. Plant Biotech Rep 2010; 4:261-267.
  26. Jayanthi M, Mandal PK. Plant regeneration through somatic embryogenesis and RAPD analysis of regenerated plants in Tylophora indica (Burm. f. Merrill.). In vitro Cell Dev Biol Plant 2001; 37:576-580.
  27. George EF, Hall MA, De Klerk GJ. Plant propagation by tissue culture: Micropropagation: uses and methods. Springer, Netherla, 2008.
  28. Ilahi I, Ghauri EG. Regeneration in cultures of Papaver bracteatum as influenced by growth hormones and temperature. Pctoc J 1994; 38:81-83.
  29. Farjaminezhad R, Zare N, Asghai-Zakari R, Farjaminezhad M. Establishment and optimization of cell growth in suspension culture of Papaver bracteatum: a biotechnology approach for thebaine production. Turk J Biol 2013; 37:689-697.
  30. Verma AK, Singh RR, Singh S. Improved alkaloid content in callus culture of Catharanthu sroseus. Bot Serbica 2012; 36:123-130.
  31. Muthulakshmi S, Pandiyarajan V. Influence of IAA on the vincristine content of Catharanthusroseus (L). G. Don. Asian J Plant Sci Res. 2013; 3:81-87.
  32. Almagro L, Fernandez-Perez F, Pedreno MA. Indole alkaloids from Catharanthus roseus: bioproduction and their effect on human health. Molecules 2015; 20:2973–3000.
  33. Mostafa GG, Alhamd MA. Effect of gibberellic acid and indole 3-acetic acid on improving growth and accumulation of phytochemical composition in Balanites aegyptiaca plants. AJPS 2011; 6:36-43.
  34. Vendrame WA, Faria RT. Phloroglucinol enhances recovery and survival of cryopreserved Dendrobium nobileprotocorms. Sci Hortic 2011; 128:131-135.
  35. Sarkar D, Naik PS. Phloroglucinol enhances growth and rate of axillary shoot proliferation in potato shoot tip cultures in vitro. Pctol J 2000; 60:139-149.
  36. Ying-Ning Z. Micropropagation of Chinese Plum (Prunus salicina Lindl.) using mature stem segments. Not Bot HortiAgrobot Cluj-Na 2010; 38:214-218.
  37. Find J, Grace L, Krogstrup P. Effect of anti"auxins on maturation of embryogenic tissue cultures of Nordmanns fir (Abies nordmanniana). Physiol Plant 2002; 116:231–237.
  38. Pages G, Lenormand P, L'Allemain G, Chambard JC, Meloch S, Pouyssegur J. Mitogen-activated protein kinases p42mapk and p44mapk are required for fibroblast proliferation. Proc Natl Acad Sci 1993; 90:8319-8323.
  39. Kim JA, Lee JM, Shin DB, Lee NH. The antioxidant activity and tyrosinase inhibitory activity of phloro-tannins in Ecklonia cava. Food Sci Biotechnol 2004; 13:476-480.
  40. Mittler R, Vanderauwera S, Gollery M, Van Breusegem F.There active oxygen gene network in plants. Trends Plant Sci 2004; 9:490–498.
  41. Polle A. Dissecting the superoxide dismutase-ascorbate-glutathione- pathway in chloroplasts by metabolic modeling: computer simulationsas a step towards flux analysis. Plant Physiol1 2001; 26:445–462.
  42. Ketchum RE, Gibson DM, Croteau RB, Shuler ML. The kinetics of taxoid accumulation in cell suspension cultures of Taxus following elicitation with methyl jasmonate. Biotechnol Bioeng 1999; 62:97-105.
  43. Kang SM, Jung HY, Kang YM, Yun DJ, Bahk JD, Yang JK, Choi MS. Effects of methyl jasmonate and salicylic acid on the production of tropane alkaloids and the expression of PMT and H6H in adventitious root cultures of Scopolia parviflora. Plant Sci 2004; 166:745-751.
  44. Ruiz-May E, Galaz-ívalos RM, Loyola-Vargas VM. Differential secretion and accumulation of terpene indole alkaloids in hairy roots of Catharanthus roseus treated with methyl jasmonate. Mol Biotech 2009; 41:278.
  45. Deng F. Effects of glyphosate, chlorsulfuron, and methyl jasmonate on growth and alkaloid biosynthesis of jimsonweed (Datura stramonium L.). Pestic Biochem Physiol 2005; 82:16–26.
  46. Sudha G, Ravishankar GA. Involvement and interaction of various signaling compounds on the plant metabolic events during defense response, resistance to stress factors, formation of secondary metabolites and their molecular aspects. Pctoc J 2002; 71:181-212.
  47. Khosroushahi AY, Valizadeh M, Ghasempour A, Khosrowshahli M, Naghdibadi H, Dadpour MR, Omidi Y. Improved Taxol production by combination of inducing factors in suspension cell culture of Taxusbaccata. Cell Biol Int. 2006; 30:262-269.
  48. Qu J, Zhang W, Yu X. A combination of elicitation and precursor feeding leads to increased anthocyanin synthesis in cell suspension cultures of Vitis vinifera. Pctoc J 2011; 107:261-269.
  49. Färber K, Schumann B, Miersch O, Roos W. Selective desensitization of jasmonate-and pH-dependent signaling in the induction of benzophenanthridine biosynthesis in cells of Eschscholzia californica. Phytochem 2003; 62:491-500.
Read More
Author biographies is not available.
Crossref
Scopus
Google Scholar
Europe PMC
Download this PDF file
PDF
Statistic
Read Counter : 844 Download : 507