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Vol. 66 No. 6: Issue 6

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The expression levels of Mir-146b and Mir-221 in thyroid carcinoma tissues and their correlation with malignancy degree

https://doi.org/10.14715/cmb/2020.66.6.25
Dandan Tan
Heilongjiang Province Hospital, Nangang Campus, Harbin, 150001, China
Yan Cui
Heilongjiang Province Hospital, Nangang Campus, Harbin, 150001, China
Jing Bao
Heilongjiang Province Hospital, Nangang Campus, Harbin, 150001, China
Fenlan Xu
Department of Anesthesiology, The Public Health Clinical Center of Chengdu, Chengdu, 610066, China
Junfeng Ma
Heilongjiang Province Hospital, Nangang Campus, Harbin, 150001, China

Corresponding Author(s) : Junfeng Ma

majunfeng452@163.com

Cellular and Molecular Biology, Vol. 66 No. 6: Issue 6

Abstract

This experiment aimed to investigate the correlation between the expressions of mir-146b and mir-221 and the proliferation, invasion, and malignancy of tumor cells. For this purpose, 135 patients with thyroid cancer treated in our hospital and 120 patients with non-malignant thyroid cancer in the outpatient clinic were selected as subjects. Fine needle biopsy tissues of the two groups were taken as samples to detect the expression levels of mir-146b and mir-221 in the tissues. The effect of the two microRNA on the proliferation and migration of cancer cells was observed by the transfection of cell lines. The contents of gal-3 and MMP9 in serum were further detected, and their relationship with the expressions of mir-146b and mir-221 was analyzed. The results showed that the expressions of mir-146b and mir-221 were significantly decreased and increased in thyroid carcinoma fine-needle aspiration tissues. Mir-146b inhibited the proliferation and migration of cancer cells, while mir-221 promoted this process. In the more cancer cells, the expression levels of these two genes changed more, and the serum levels of gal-3 and MMP9 also increased. It was concluded that the expression levels of mir-146b and mir-221 were correlated with the degree of tumor malignancy, and the low expression of mir-146b and the high expression of mir-221 were correlated with the proliferation, invasion, and malignancy of thyroid cancer tumor cells.

Keywords

Mir-146b Mir-221 Thyroid carcinoma tissues Malignancy degree.
Tan, D., Cui, Y., Bao, J., Xu, F., & Ma, J. (2020). The expression levels of Mir-146b and Mir-221 in thyroid carcinoma tissues and their correlation with malignancy degree. Cellular and Molecular Biology, 66(6), 142–147. https://doi.org/10.14715/cmb/2020.66.6.25
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