Issue

Vol. 65 No. 5: Issue 5

The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.

Protective and therapeutic effects of Pilose antler against kidney deficiency-induced osteoporosis

Cong Ren
Department of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, Liaoning, China
Wei Gong
Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang, Liaoning, China
Feng Li
Department of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, Liaoning, China
Ming Xie
Department of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, Liaoning, China

Corresponding Author(s) : Feng Li

sfyb39@163.com

Cellular and Molecular Biology, Vol. 65 No. 5: Issue 5

Abstract

This study was carried out to evaluate the preventive and curative effects of Pilose antler against osteoporosis due to kidney deficiency, and investigate its potential mechanism of action. A model of osteoporosis due to kidney deficiency was established in rats using bilateral ovariectomy. Pilose antler polypeptide (PAP), Pilose antler polysaccharide (PAP'), and their mixture (PAP+PAP') were separately administered to the rats for 12 weeks, with progynova and xianlingubao tablets (XLGB) as the positive control groups. We determined the bone mineral density (BMD) and uterus Index of the rats. Osteoblastic bone metabolism-related indices in serum and bone tissue were measured with ELISA. Western blotting and RT-PCR were used to investigate the protein and mRNA expressions of Bmp-2, Smad1, Smad5, Runx2 in bone tissue. The morphology of bone tissue was determined using immunohistochemical methods. Compared with control group, PAP, PAP', PAP+PAP' increased BMD and regulated bone metabolism indices in serum and bone tissue. Treatment with Pilose antler up-regulated the mNRA and protein expressions of Bmp-2, Smad1, Smad5 and Runx2. Immunohistochemical staining showed that Bmp-2, Smad1, Smad5 and Runx2 were stained brown, indicating that all of them were positive. There were abnormal changes in the protein expressions of Bmp-2, Smad1, Smad5 and Runx2 in bone tissue, which may be an important mechanism underlying the development of kidney deficiency osteoporosis. Moreover, PAP, PAP' and PAP+PAP' had some preventive effects on osteoporosis, probably via the activation of the Bmp-2/Smad1 and Smad5/Runx2 signaling pathways through induction of high expressions of their mRNAs and proteins.

Keywords

Pilose antler polypeptide Pilose antler polysaccharide Osteoporosis Kidney deficiency Traditional Chinese Medicine (TCM).
Ren, C., Gong, W., Li, F., & Xie, M. (2019). Protective and therapeutic effects of Pilose antler against kidney deficiency-induced osteoporosis. Cellular and Molecular Biology, 65(5), 24–31. Retrieved from https://mail.cellmolbiol.org/index.php/CMB/article/view/2968
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. National Pharmacopoeia Committee, Volume One, Chinese Pharmacopoeia, 2015 Edition, Beijing, The Medicine Science and Technology Press of China, 2015, pp. 323-324.
  2. Gong W. Study on the relationship between the role of "tonifying the kidney and strengthening bone" and the quality of the Pilose Antler. Liaoning Univ Tradit Chin Med 2011.
  3. Ye J, Coulouris G, Zaretskaya I, Cutcutache I, Rozen S, Madden TL. Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction. BMC Bioinform 2012; 13: 134.
  4. Pfaffl MW. A new mathematical model for relative quantification in real-time RT–PCR. Nucleic Acids Res 2001; 29: 2002-2007.
  5. Kobune M, Kato J, Chiba H, Kawano Y, Tanaka M, Takimoto R, et al. Telomerized human bone marrow-derived cell clones maintain the phenotype of hematopoietic-supporting osteoblastic and myofibroblastic stromal cells after long-term culture. Exp Hematol 2005; 33: 1544-1553.
  6. Komori T. Regulation of bone development and maintenance by Runx2. Front Biosci 2008; 13: 898-903.
  7. Gaur T, Hussain S, Mudhasani R, Parulkar I, Colby JL, Frederick D, et al. Dicer inactivation in osteoprogenitor cells compromises fetal survival and bone formation, while excision in differentiated osteoblasts increases bone mass in the adult mouse. Dev Biol 2010; 340: 10-21.
  8. Hu ZM, Peel SA, SK HO, Sandor GK, Clokie CM. Induction of bone matrix protein expression by native bone matrix proteins in C2C12 culture. Biomed Environ Sci 2009; 22: 164-169.
  9. Smoljanovic T, Bojanic I, Bogovic M. Bone morpho-genetic protein. J Neurosurg Spine 2012; 11: 92-93.
  10. Lo KW, Ulery BD, Ashe KM, Laurencin CT. Studies of bone morphogenetic protein-based surgical repair. Adv Drug Deliv Rev 2012; 64: 1277-1291.
  11. Kato S, Kawabata N, Suzuki N, Ohmura M, Takagi M. Bone morphogenetic protein-2 induces the differentia-tion of a mesenchymal progenitor cell line, ROB-C26, into mature osteoblasts and adipocytes. Life Sci 2009; 84: 302-310.
  12. Karageorgiou V, Meinel L, Hofmann S, Malhotra A, Volloch V, Kaplan D. Bone morpho-genetic protein-2 decorated silk fibroin films induce osteogenic differentiation of human bone marrow stromal cells. J Biomed Mater Res A 2004; 71: 528-537.
  13. Tachi K, Takami M, Zhao B, Mochizuki A, Yamada A, Miyamoto Y, et al. Bone morphogenetic protein 2 enhances mouse osteoclast differentiation via increased levels of receptor activator of NF-κB lig and expression in osteoblasts. Cell Tissue Res 2010; 342: 213-220.
  14. Cao X, Chen D. The BMP signaling and in vivo bone formation. Gene 2005; 357: 1-8.
  15. ten Dijke P. Bone morphogenetic protein signal transduction in bone. Curr Med Res Opin 2006; 22: 7-11.
  16. Franceschi RT, Ge C, Xiao G, Roca H, Jiang D. Transcriptional Regulation of Osteoblasts. Cells Tissues Organs 2009; 189: 144-152.
  17. Gong W, Li F. Cervi cornu pantotrichun aqueous extract promote osteoblasts differentia-tion and bone formation. Biomed Res 2014; 25: 249-252.
  18. Yeung DKW, Griffith JF, Antonio GE, Lee FKH, Woo J, Leung PC. Osteoporosis is associated with increased marrow fat content and decreased marrow fat unsaturation: a proton MR spectroscopy study. J Magn Reson Imaging 2010; 2: 279-285.
  19. Li CQ. Screening for the anti-inflammatory activity of frac-tions and compounds from Atractylodesmacrocephala koidz. J Ethnopharmacol 2007; 114: 212-217.
  20. JC Lee, KY Lee, YO Son, KC Choi, J Kim, SH Kim, et al. Stimulating effects on mouse Spleuocytes of glyco-proteins from the herbal medicine Atractylodes macrocepha-la Koidz. Phytomed 2007; 14: 390-395.
  21. Shi CD, Yang YH, Yu X, Zhang RQ, Wang XL, Nie AD. Effect of Kangshu Jiangu Granules on the Expression of PPARγ-2 of Bone Tissue from Ovariectomized Osteoporosis Model Rats. J Sichuan Tradit Chin Med 2015; 33: 61-64.
  22. Hu J, Zhang AB, Liu XL, Zhou JN. Extracorporeal shock wave promotes differentiation of human bone marrow stromal cells into osteoprogenitors. Chin J Exp Surg 2005; 22: 147-150.
  23. Sun WP, Li FS, Chen C, Luo H, Yang G, Liu H. Immunomodulation of Atractylodis polysaccharides in mice. Chin J Microecol 2011; 23: 881-882, 886.
  24. Kim K, Dean D, Wallace J, Breithaupt R, Mikos AG, Fisher JP. The influence of stereolithographic scaffold architecture and composition on osteogenic signal expression with rat bone marrow stromal cells. Biomater 2001; 32: 3750.
  25. Ren YL, Li YL, Lv HB, Liu LP, Zhao JR, Wang LC. Expressions of TGF-β1/Smad4 mRNA of Kidney by Zuogui Pill in Ovariectomy-induced Osteoporosis Rats. Chin J Exp Tradit Med Form 2012; 10: 190-194.
  26. Liu ZH. Osteoporosis (first edition). Science Press, Beijing, 1998, pp. 142.
  27. Li HF, Zhou WS. Progress in the pathogenesis of osteoporosis in Chinese and Western medicine. Mod Clin Med Bioeng Mag 2003; 9: 450-455.
  28. Wang HF, Weng SF. Bone mineral density measurement in rats and its application in the establishment of osteoporosis model. Chin J Osteoporos 1995; 2: 8-9
  29. Lindsay R, Tohme JF. Estrogen treatment of patients with established postmenopausal osteoporpsis. Obstet Gynecol 1990; 76: 290-295.
Read More
Author biographies is not available.
Download this PDF file
PDF
Statistic
Read Counter : 752 Download : 427