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Vol. 65 No. 5: Issue 5

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The effect of Heweijiangni-decoction on esophageal morphology in a rat model of OVA-induced visceral hypersensitivity followed by acid exposure

https://doi.org/10.14715/cmb/2019.65.5.12
Bo-yi Jia
Department of Gastroenterology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China
Chun-e Xie
Department of Gastroenterology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China
Zhi-bin Wang
Department of Gastroenterology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China
Wen-jing Pei
Department of Gastroenterology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China
Xiao-hong Li
Department of Gastroenterology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China
Lei Shi
Department of Gastroenterology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China
Jia-li Liu
Department of Gastroenterology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China
Ya-fei Han
Graduate School, Beijing University of Chinese Medicine, Beijing 100029, China
Xiang Tan
Department of Gastroenterology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China
Pang-hua Ding
Department of Gastroenterology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China
Zhong-mei Sun
Department of Gastroenterology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China
Wen-jing Yuan
Department of Gastroenterology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China
Jun-xiang Li
Department of Gastroenterology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China

Corresponding Author(s) : Jun-xiang Li

bpih94@163.com

Cellular and Molecular Biology, Vol. 65 No. 5: Issue 5

Abstract

Heweijiangni decoction (HWJND) is an effective traditional Chinese medicine prescription in clinical treatment of nonerosive reflux disease (NERD). Esophageal hypersensitivity and acid contribute to the disease. However, the exact underlying mechanism of action remains unclear. In this study, we observed the effect of HWJND on esophageal morphology in a rat model of ovalbumin (OVA)-induced visceral hypersensitivity followed by acid exposure. Esophageal morphology was assessed by measuring the extent of dilated intercellular spaces (DIS), desmosome disruption, and mitochondrial fragmentation. HWJND in low, moderate, and high doses relieved DIS and desmosome disruption in esophageal epithelium compared with model group (P<0.05 for all doses). In addition, HWJND in high dose protected mitochondria from fragmentation (P<0.05). Other findings suggest that DIS and mitochondrial fragmentation are independent events, and that omeprazole protects mitochondria. Overall, HWJND significantly resists esophageal morphology changes in OVA-induced and acid exposure rat model.

Keywords

Heweijiangni decoction (HWJND) Gastroesophageal reflux disease OVA.
Jia, B.- yi, Xie, C.- e, Wang, Z.- bin, Pei, W.- jing, Li, X.- hong, Shi, L., Liu, J.- li, Han, Y.- fei, Tan, X., Ding, P.- hua, Sun, Z.- mei, Yuan, W.- jing, & Li, J.- xiang. (2019). The effect of Heweijiangni-decoction on esophageal morphology in a rat model of OVA-induced visceral hypersensitivity followed by acid exposure. Cellular and Molecular Biology, 65(5), 73–78. https://doi.org/10.14715/cmb/2019.65.5.12
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