Urtica dioica agglutinin (a plant lectin) has a caspase-dependent apoptosis induction effect on the acute lymphoblastic leukemia cell line

Azam Rashidbaghan; Ali Mostafaie; Yaghoub Yazdani; Kamran Mansouri

doi:10.14715/cmb/2020.66.6.22

Issue

Vol. 66 No. 6: Issue 6

Issue Published : October 6, 2020

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Urtica dioica agglutinin (a plant lectin) has a caspase-dependent apoptosis induction effect on the acute lymphoblastic leukemia cell line

https://doi.org/10.14715/cmb/2020.66.6.22

Azam Rashidbaghan

Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran

Ali Mostafaie

Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran

Yaghoub Yazdani

Stem Cell Research Center, Golestan University of Medical Sciences, Gorgan, Iran

Kamran Mansouri

Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran

Corresponding Author(s) : Ali Mostafaie

ali_mostafaie@yahoo.com

Cellular and Molecular Biology, Vol. 66 No. 6: Issue 6
Article Published : September 30, 2020

Abstract

Urtica dioica agglutinin (UDA) is a very small plant lectin with anti-prostatic activity. In this study, we investigated the effect of UDA on proliferation and apoptosis induction in human acute lymphoid leukemia (ALL) cell lines. The effect of UDA on Jurkat and Raji cell proliferation was examined by MTS assay. Distribution of cell cycle phases was determined by PI staining and apoptosis was examined with annexin V/PI and western blot. Results showed UDA treatment reduced cell proliferation in cells by inducing apoptosis. PI staining was associated with a higher percentage of the cell population in sub G1. Caspase-8 and caspase-9 dependent apoptosis occurred in Jurkat cells. Generally, UDA treatment resulted in cell death in ALL cell lines and induced apoptosis in the T-ALL cell line, Jurkat, through extrinsic and intrinsic pathways. These results may be considered as a guide to working on UDA as an anti-leukemic drug in the future.

Keywords

Acute lymphoid leukemia Urtica dioica agglutinin Lectin Anti-leukemic drug.

Rashidbaghan, A., Mostafaie, A., Yazdani, Y., & Mansouri, K. (2020). Urtica dioica agglutinin (a plant lectin) has a caspase-dependent apoptosis induction effect on the acute lymphoblastic leukemia cell line. Cellular and Molecular Biology, 66(6), 121–126. https://doi.org/10.14715/cmb/2020.66.6.22

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References

Peumanus WJ, Ley MD, Broekaert WF. An unusual lectin from stinging nettle (Urtica dioica) rhizomes. FEBS Lett 1984;177(1):99-103.
Beintema JJ, Peumans WJ. The primary structure of stinging nettle (Urtica dioica) agglutinin a two-domain member of the hevein family. FEBS Lett 1992;299(2):131-4.
Galelli A, Truffa-Bachi P. Urtica dioica agglutinin. A superantigenic lectin from stinging nettle rhizome. J Immunol 1993;151(4):1821-31.
Saul FA, Rovira P, Boulot G, Damme EJV, Peumans WJ, Truffa-Bachi P, et al. Crystal structure of Urtica dioica agglutinin, a superantigen presented by MHC molecules of class I and class II. Structure 2000;8:593-603.
Mejía EGD, Prisecaru VI. Lectins as bioactive plant proteins: a potential in cancer treatment. Crit Rev Food Sci Nutr 2005;45(6):425-45.
Does MP, Ng DK, Dekker HL, Peumans WJ, Houterman PM, Damme EJMV, et al. Characterization of Urtica dioica agglutinin isolectins and the encoding gene family. Plant Mol Biol 1999;39(2):335-47.
Wagner H, Geiger M, Boos G, Samtleben R. Studies on the binding of Urtica dioica agglutinin (UDA) and other lectins in an in vitro epidermal growth factor receptor test. Phytomedicine 1995;4:287-90.
Hryb D, Khan M, Romas N, Rosner W. The effect of extracts of the roots of the stinging nettle (Urtica dioica) on the interaction of SHBG with its receptor on human prostatic membranes. Planta Med 1995;61(1):31-2.
Lichius J, Muth C. The inhibiting effects of Urtica dioica root extracts on experimentally induced prostatic hyperplasia in the mouse. Planta Med 1997;63(4):307-10.
Konrad L, Muller H-H, Lenz C, Laubinger H, Aumuller G, Lichius JJ. Antiproliferative effect on human prostate cancer cells by a stinging nettle root (Urtica dioica) extract. Planta Med 2000;66(1):44-7.
Rodriguez-Abreu D, Bordoni A, Zucca E. Epidemiology of hematological malignancies. Ann Oncol 2007. Ann Oncol. 2007;18(Suppl 1):i3-i8.
Narayanan S, Shami PJ. Treatment of acute lymphoblastic leukemia in adults. Crit Rev Oncol Hematol 2012;81:84-102.
Gutierrez A, Look AT. Molecular targeted therapies in T-cell acute lymphoblastic leukemia. In: RJ HPaA, editor. Molecularly Targeted Therapy for Childhood Cancer. LLC: Springer Science+Business Media; 2010. p. 19-30.
Fridlender M, Kapulnik Y, Koltai H. Plant derived substances with anti-cancer activity: from folklore to practice. Front Plant Sci 2015;6:799.
Ferriz-Martinez RA, Torres-Arteaga IC, Blanco-Labra A, Garcia-Gasca T. The role of plant lectins in cancer treatment. In: Vazquez DMDCM NS, editor. New approaches in the treatment of cancer. Mexico: Nova Science Publishers; 2010. p. 71-89.
Katiyar S, Damme EJMV, Peumans WJ, Surolia A. Thermodynamic analysis of chitooligosaccharide binding to Urtica dioica agglutinin by isothermal titration calorimetry. Bioscience Rep 1999;19(5):411-9.
Liener I. The photometric determination of the hemaglutination activity of soyin and crude soybean extracts. Arch Biochem Biophys. 1955;54(1):223-31.
Kaul R, Read J, Mattiasson B. Screening for plant lectins by latex agglutination tests. Phytochemistry 1991;30(12):4005-9.
Nilsson C, Aboud S, Karlén K, Hejdeman B, Urassa W, Biberfeld G. Optimal blood mononuclear cell isolation procedures for gamma interferon enzyme-linked immune spot testing of healthy Swedish and Tanzanian subjects. CVI 2008;15(4):585-9.
Looi C, Arya A, Cheah F, Muharram B, Leong K, Mohamad K, et al. Induction of apoptosis in human breast cancer cells via caspase pathway by vernodalin isolated from Centratherum anthelminticum (L.) seeds. PLOS ONE 2013;8(2):e56643.
Bradford M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72:248-54.
Attali G, Gancz D, Fishelson Z. Increased sensitivity of early apoptotic cells to complement-mediated lysis. Eur J Immunol 2004;34:3236-45.
Kawabata BY, Hirokawa M, Kitabayashi A, Horiuchi T, Kuroki J, Miura AB. Defective apoptotic signal transduction pathway downstream of caspase-3 in human B-lymphoma cells: A novel mechanism of nuclear apoptosis resistance. Blood. 1999;94(10):3523-30.
Luciano F, Ricci J, Herrant M, Mari B, Cousin J, Auberger P. T and B leukemic cell lines exhibit different requirements for cell death: correlation between caspase activation, DFF40/DFF45 expression, DNA fragmentation and apoptosis in T cell lines but not in Burkitt's lymphoma. Leukemia 2002;16:700-7.
Broker LE, Kruyt FAE, Giaccone G. Cell death independent of caspases: a review. Clin Cancer Res 2005;11(9):3155-62.
McIlwain DR, Berger T, Mak TW. Caspase functions in cell death and disease. Cold Spring Harb Perspect Biol 2013;5(4):a008656.
Liu B, Bian H-j, Bao J-k. Plant lectins: potential antineoplastic drugs from bench to clinic. Cancer Lett 2010;287(1):1-12.
Azimi S, Mozdarani H, Mahmoudzadeh A. Induction of DNA damage, apoptosis and micronuclei in peripheral blood lymphocytes following injection of contrast media in patients with abdominal CT scan. Int J Radiat Res. 2017; 15 (2) :149-155.
Mortazavi S, Erfani N, Mozdarani H, Azmoonfar R, Shokrpour N. Induction of apoptosis by 900 MHz radiofrequency radiation emitted from a GSM mobile phone simulator in bystander Jurkat cells. Int J Radiat Res. 2015; 13 (2) :181-186.
Haeri G, Rajabi H, Akhlaghpoor S. SPIO-Annexin V, a potential probe for MRI detection of radiation induced apoptosis. Int J Radiat Res. 2014; 12 (3) :217-222.
Shahidi M, Mozdarani S, Shammas S. Interindividual differences in radiation-induced apoptosis of peripheral blood leukocytes in normal individuals and breast cancer patients. Int J Radiat Res. 2012; 9(4): 237-244.
Ghoraeia, P, Mozdarani H, Akhlaghpoor S. Effect of famotidine on radiation induced apoptosis in human peripheral blood leukocytes. Int J Radiat Res. 2005; 3 (1) :31-36.
Rezakhani N, Goliaei B, Parivar K, Nikoofar A. Effects of X-irradiation and sinensetin on apoptosis induction in MDA-MB-231 human breast cancer cells. Int J Radiat Res. 2020; 18 (1) :75-82.
Jamalzadeh L, Ghafoori H, Aghamaali M, Sariri R. Induction of Apoptosis in Human Breast Cancer MCF-7 Cells by a Semi-Synthetic Derivative of Artemisinin: A Caspase-Related Mechanism. Iran J Biotechnol. 2017; 15(3): 157-165.
Khoshtinat Nikkhoi S, Dorostkar R, Ranjbar S, Heydarzadeh H, Tat M, Ghalavand M, Farasat A, Hashemzadeh MS. Synergistic Effect of Expressed miR-128 and Puma Protein on Targeted Induction of Tumor Cell Apoptosis. Iran J Biotechnol. Sep 2016; 14(3): 185-191.
Mahdavinezhad F, Kazemi P, Fathalizadeh P, Sarmadi F, Sotoodeh L, Hashemi E, Hajarian H, Dashtizad M. In vitro versus In vivo: Development-, Apoptosis-, and Implantation- Related Gene Expression in Mouse Blastocyst. Iran J Biotechnol. Jan 2019; 17(1): e2157.

References

Peumanus WJ, Ley MD, Broekaert WF. An unusual lectin from stinging nettle (Urtica dioica) rhizomes. FEBS Lett 1984;177(1):99-103.

Beintema JJ, Peumans WJ. The primary structure of stinging nettle (Urtica dioica) agglutinin a two-domain member of the hevein family. FEBS Lett 1992;299(2):131-4.

Galelli A, Truffa-Bachi P. Urtica dioica agglutinin. A superantigenic lectin from stinging nettle rhizome. J Immunol 1993;151(4):1821-31.

Saul FA, Rovira P, Boulot G, Damme EJV, Peumans WJ, Truffa-Bachi P, et al. Crystal structure of Urtica dioica agglutinin, a superantigen presented by MHC molecules of class I and class II. Structure 2000;8:593-603.

Mejía EGD, Prisecaru VI. Lectins as bioactive plant proteins: a potential in cancer treatment. Crit Rev Food Sci Nutr 2005;45(6):425-45.

Does MP, Ng DK, Dekker HL, Peumans WJ, Houterman PM, Damme EJMV, et al. Characterization of Urtica dioica agglutinin isolectins and the encoding gene family. Plant Mol Biol 1999;39(2):335-47.

Wagner H, Geiger M, Boos G, Samtleben R. Studies on the binding of Urtica dioica agglutinin (UDA) and other lectins in an in vitro epidermal growth factor receptor test. Phytomedicine 1995;4:287-90.

Hryb D, Khan M, Romas N, Rosner W. The effect of extracts of the roots of the stinging nettle (Urtica dioica) on the interaction of SHBG with its receptor on human prostatic membranes. Planta Med 1995;61(1):31-2.

Lichius J, Muth C. The inhibiting effects of Urtica dioica root extracts on experimentally induced prostatic hyperplasia in the mouse. Planta Med 1997;63(4):307-10.

Konrad L, Muller H-H, Lenz C, Laubinger H, Aumuller G, Lichius JJ. Antiproliferative effect on human prostate cancer cells by a stinging nettle root (Urtica dioica) extract. Planta Med 2000;66(1):44-7.

Rodriguez-Abreu D, Bordoni A, Zucca E. Epidemiology of hematological malignancies. Ann Oncol 2007. Ann Oncol. 2007;18(Suppl 1):i3-i8.

Narayanan S, Shami PJ. Treatment of acute lymphoblastic leukemia in adults. Crit Rev Oncol Hematol 2012;81:84-102.

Gutierrez A, Look AT. Molecular targeted therapies in T-cell acute lymphoblastic leukemia. In: RJ HPaA, editor. Molecularly Targeted Therapy for Childhood Cancer. LLC: Springer Science+Business Media; 2010. p. 19-30.

Fridlender M, Kapulnik Y, Koltai H. Plant derived substances with anti-cancer activity: from folklore to practice. Front Plant Sci 2015;6:799.

Ferriz-Martinez RA, Torres-Arteaga IC, Blanco-Labra A, Garcia-Gasca T. The role of plant lectins in cancer treatment. In: Vazquez DMDCM NS, editor. New approaches in the treatment of cancer. Mexico: Nova Science Publishers; 2010. p. 71-89.

Katiyar S, Damme EJMV, Peumans WJ, Surolia A. Thermodynamic analysis of chitooligosaccharide binding to Urtica dioica agglutinin by isothermal titration calorimetry. Bioscience Rep 1999;19(5):411-9.

Liener I. The photometric determination of the hemaglutination activity of soyin and crude soybean extracts. Arch Biochem Biophys. 1955;54(1):223-31.

Kaul R, Read J, Mattiasson B. Screening for plant lectins by latex agglutination tests. Phytochemistry 1991;30(12):4005-9.

Nilsson C, Aboud S, Karlén K, Hejdeman B, Urassa W, Biberfeld G. Optimal blood mononuclear cell isolation procedures for gamma interferon enzyme-linked immune spot testing of healthy Swedish and Tanzanian subjects. CVI 2008;15(4):585-9.

Looi C, Arya A, Cheah F, Muharram B, Leong K, Mohamad K, et al. Induction of apoptosis in human breast cancer cells via caspase pathway by vernodalin isolated from Centratherum anthelminticum (L.) seeds. PLOS ONE 2013;8(2):e56643.

Bradford M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72:248-54.

Attali G, Gancz D, Fishelson Z. Increased sensitivity of early apoptotic cells to complement-mediated lysis. Eur J Immunol 2004;34:3236-45.

Kawabata BY, Hirokawa M, Kitabayashi A, Horiuchi T, Kuroki J, Miura AB. Defective apoptotic signal transduction pathway downstream of caspase-3 in human B-lymphoma cells: A novel mechanism of nuclear apoptosis resistance. Blood. 1999;94(10):3523-30.

Luciano F, Ricci J, Herrant M, Mari B, Cousin J, Auberger P. T and B leukemic cell lines exhibit different requirements for cell death: correlation between caspase activation, DFF40/DFF45 expression, DNA fragmentation and apoptosis in T cell lines but not in Burkitt's lymphoma. Leukemia 2002;16:700-7.

Broker LE, Kruyt FAE, Giaccone G. Cell death independent of caspases: a review. Clin Cancer Res 2005;11(9):3155-62.

McIlwain DR, Berger T, Mak TW. Caspase functions in cell death and disease. Cold Spring Harb Perspect Biol 2013;5(4):a008656.

Liu B, Bian H-j, Bao J-k. Plant lectins: potential antineoplastic drugs from bench to clinic. Cancer Lett 2010;287(1):1-12.

Azimi S, Mozdarani H, Mahmoudzadeh A. Induction of DNA damage, apoptosis and micronuclei in peripheral blood lymphocytes following injection of contrast media in patients with abdominal CT scan. Int J Radiat Res. 2017; 15 (2) :149-155.

Mortazavi S, Erfani N, Mozdarani H, Azmoonfar R, Shokrpour N. Induction of apoptosis by 900 MHz radiofrequency radiation emitted from a GSM mobile phone simulator in bystander Jurkat cells. Int J Radiat Res. 2015; 13 (2) :181-186.

Haeri G, Rajabi H, Akhlaghpoor S. SPIO-Annexin V, a potential probe for MRI detection of radiation induced apoptosis. Int J Radiat Res. 2014; 12 (3) :217-222.

Shahidi M, Mozdarani S, Shammas S. Interindividual differences in radiation-induced apoptosis of peripheral blood leukocytes in normal individuals and breast cancer patients. Int J Radiat Res. 2012; 9(4): 237-244.

Ghoraeia, P, Mozdarani H, Akhlaghpoor S. Effect of famotidine on radiation induced apoptosis in human peripheral blood leukocytes. Int J Radiat Res. 2005; 3 (1) :31-36.

Rezakhani N, Goliaei B, Parivar K, Nikoofar A. Effects of X-irradiation and sinensetin on apoptosis induction in MDA-MB-231 human breast cancer cells. Int J Radiat Res. 2020; 18 (1) :75-82.

Jamalzadeh L, Ghafoori H, Aghamaali M, Sariri R. Induction of Apoptosis in Human Breast Cancer MCF-7 Cells by a Semi-Synthetic Derivative of Artemisinin: A Caspase-Related Mechanism. Iran J Biotechnol. 2017; 15(3): 157-165.

Khoshtinat Nikkhoi S, Dorostkar R, Ranjbar S, Heydarzadeh H, Tat M, Ghalavand M, Farasat A, Hashemzadeh MS. Synergistic Effect of Expressed miR-128 and Puma Protein on Targeted Induction of Tumor Cell Apoptosis. Iran J Biotechnol. Sep 2016; 14(3): 185-191.

Mahdavinezhad F, Kazemi P, Fathalizadeh P, Sarmadi F, Sotoodeh L, Hashemi E, Hajarian H, Dashtizad M. In vitro versus In vivo: Development-, Apoptosis-, and Implantation- Related Gene Expression in Mouse Blastocyst. Iran J Biotechnol. Jan 2019; 17(1): e2157.

Author biographies is not available.