Expression of miR-93-5p in patients with esophageal carcinoma and its relationship with the curative effect and prognosis of radiotherapy

Lingzhi Chen; Xinhua Fan; Xiangming Wang; Xianhua Wu

doi:10.14715/cmb/2020.66.2.6

Issue

Vol. 66 No. 2: Issue 2

Issue Published : May 16, 2020

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Expression of miR-93-5p in patients with esophageal carcinoma and its relationship with the curative effect and prognosis of radiotherapy

https://doi.org/10.14715/cmb/2020.66.2.6

Lingzhi Chen

Department of Radiology, Yiwu Central Hospital, Yiwu 322000, P.R. China

Xinhua Fan

Department of Radiology, Yiwu Central Hospital, Yiwu 322000, P.R. China

Xiangming Wang

Department of Radiology, Yiwu Central Hospital, Yiwu 322000, P.R. China

Xianhua Wu

Department of Ultrasonography, Yiwu Central Hospital, Yiwu 322000, P.R. China

Corresponding Author(s) : Xianhua Wu

yonggang@list.ru, xhfn74@163.com

Cellular and Molecular Biology, Vol. 66 No. 2: Issue 2
Article Published : May 15, 2020

Abstract

This study aimed to investigate the expression of miR-93-5p in esophageal carcinoma-patients and its relationship with the curative effect and prognosis of radiotherapy. 102 patients with esophageal carcinoma treated in Yiwu Central Hospital from May 2013 to July 2015 were considered as the experimental group, and 89 healthy people for physical examination during the same period were selected as the control group. The expression of miR-93-5p in the serum of the two groups was compared. Based on the mean expression of miR-93-5p in serum, esophageal carcinoma patients were divided into high expression and low expression groups. Then the relationship between clinical-pathological characteristics and the expression of miR-93-5p was analyzed. The curative effect of radiotherapy in patients with esophageal carcinoma was evaluated, and the relationship between the expression of miR-93-5p, the curative effect of radiotherapy and the 3-year survival rate in patients with esophageal carcinoma was analyzed. The expression of miR-93-5p in the serum of the experimental group was significantly higher than that of the control group (P< 0.05); there was a significant correlation between the expression of miR-93-5p and pathological stage (P< 0.05). The expression of miR-93-5p in the effective radiotherapy group was significantly lower than that in the ineffective radiotherapy group (P< 0.05). ROC curve showed that the sensitivity and specificity of miR-93-5p in predicting radiotherapy response of esophageal carcinoma were 88.57 and 64.69% respectively, AUC was 0.864 (95%CI:0.791~0.936), P< 0.001; the 3-year survival rate of low expression group was significantly higher than that of high expression group (P< 0.05). In Conclusion, the expression of miR-93-5p was high in esophageal carcinoma patients, and the higher the expression, the worse the curative effect of radiotherapy and the worse the prognosis, which may be a new predictor of radiotherapy effect and prognosis in patients with esophageal carcinoma.

Keywords

miR-93-5p Esophageal carcinoma Radiotherapy.

Chen, L., Fan, X., Wang, X., & Wu, X. (2020). Expression of miR-93-5p in patients with esophageal carcinoma and its relationship with the curative effect and prognosis of radiotherapy. Cellular and Molecular Biology, 66(2), 41–46. https://doi.org/10.14715/cmb/2020.66.2.6

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References

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Wang L, Lin Q, Yang T, Liang Y, Nie Y, Luo Y and Luo F: Oryza¬nol modifies high fat diet-induced obesity, liver gene expression pro¬file, and inflammation response in mice. J Agri Food Chem 65(38): 8374-8385, 2017.
Lou Y, Shi J, Guo D, Qureshi AK and Song L: Function of PD-L1 in antitumor immunity of glioma cells. Saudi J Boil Sci 24(4): 803-807, 2017.
Guo T, Lin Q, Li X, Nie Y, Wang L, Shi L and Luo F: Octacosa¬nol attenuates inflammation in both RAW264. 7 macrophages and a mouse model of colitis. J Agri Food Chem 65(18): 3647-3658, 2017.
Li W, Jia MX, Wang JH, Lu JL, Deng J, Tang JX and Liu C: As¬sociation of MMP9-1562C/T and MMP13-77A/G polymorphisms with non-small cell lung cancer in southern Chinese population. Biomol 9(3): 107-119, 2019.
Lv Y, Zhang J and Qiao L: Quality of life in patients with esopha¬geal cancer receiving definitive chemoradiotherapy or esophagec¬tomy. Mol Clin Oncol 2014; 2: 870-874.
Nie Y, Luo F, Wang L, Yang T, Shi L, Li X, Shen J, Xu W, Guo T and Lin Q: Anti-hyperlipidemic effect of rice bran polysaccharide and its potential mechanism in high-fat diet mice. Food Func 8(11): 4028-4041, 2017.
Lou Y, Yang J, Wang L, Chen X, Xin X and Liu Y: The clini¬cal efficacy study of treatment to Chiari malformation type I with syringomyelia under the minimally invasive surgery of resection of Submeningeal cerebellar Tonsillar Herniation and reconstruction of Cisterna magna. Saudi J Biol Sci 26(8): 1927-1931, 2019.
Lou Y, Guo D, Zhang H and Song L: Effectiveness of mesenchy¬mal stems cells cultured by hanging drop vs. conventional culturing on the repair of hypoxic-ischemic-damaged mouse brains, measured by stemness gene expression. Open Life Sci 11(1): 519-523, 2016.
Chen X, Xu Y, Meng L, Chen X, Yuan L, Cai Q, Shi W and Huang G: Non-parametric partial least squares–discriminant analy-sis model based on sum of ranking difference algorithm for tea grade identification using electronic tongue data identify tea grade using e-tongue data. Sens Actuators B Chem 127924, 2020.
Yamamoto J, Hayashi T, Izumisawa Y, Kimura J, Takagawa R, Kosaka R, Ono H, Makino H, Tsuburaya A, Akiyama H, et al: [Clini¬cal Experience of Nutritional Support in Patients Treated with Che¬moradiotherapy for Locally Advanced Esophageal Cancer]. Gan To Kagaku Ryoho 2015; 42: 1246-1248.
Lloyd S and Chang BW: Current strategies in chemoradiation for esophageal cancer. J Gastrointest Oncol 2014; 5: 156-165.
Nie Y, Luo F and Lin Q: Dietary nutrition and gut microflora: A promising target for treating diseases. Trends Food Sci Technol 75: 72-80, 2018.
Ren Y, Jiao X and Zhang L: Expression level of fibroblast growth factor 5 (FGF5) in the peripheral blood of primary hypertension and its clinical significance. Saudi J Biol Sci 25(3): 469-473, 2018.
Hui AB, Lenarduzzi M, Krushel T, Waldron L, Pintilie M, Shi W, Perez-Ordonez B, Jurisica I, O'Sullivan B, Waldron J, et al: Com¬prehensive MicroRNA profiling for head and neck squamous cell carcinomas. Clin Cancer Res 2010; 16: 1129-1139.
Igarashi H, Kurihara H, Mitsuhashi K, Ito M, Okuda H, Kanno S, Naito T, Yoshii S, Takahashi H, Kusumi T, et al: Association of MicroRNA-31-5p with Clinical Efficacy of Anti-EGFR Therapy in Patients with Metastatic Colorectal Cancer. Ann Surg Oncol 2015; 22: 2640-2648.
Winther M, Alsner J, Tramm T, Baeksgaard L, Holtved E and Nordsmark M: Evaluation of miR-21 and miR-375 as prognostic biomarkers in esophageal cancer. Acta Oncol 2015; 54: 1582-1591.
Ohta K, Hoshino H, Wang J, Ono S, Iida Y, Hata K, Huang SK, Colquhoun S and Hoon DS: MicroRNA-93 activates c-Met/PI3K/ Akt pathway activity in hepatocellular carcinoma by directly inhibi¬ting PTEN and CDKN1A. Oncotarget 2015; 6: 3211-3224.
Qu MH, Han C, Srivastava AK, Cui T, Zou N, Gao ZQ and Wang QE: miR-93 promotes TGF-beta-induced epithelial-to-mesenchy¬mal transition through downregulation of NEDD4L in lung cancer cells. Tumour Biol 2016; 37: 5645-5651.
Tang Q, Zou Z, Zou C, Zhang Q, Huang R, Guan X, Li Q, Han Z, Wang D, Wei H, et al: MicroRNA-93 suppress colorectal cancer de¬velopment via Wnt/beta-catenin pathway downregulating. Tumour Biol 2015; 36: 1701-1710.
Deng W and Lin SH: Advances in radiotherapy for esophageal cancer. Ann Transl Med 6: 79, 2018
Cao Q, Liu F, Ji K, Liu N, He Y, Zhang W and Wang L: Mi¬croRNA-381 inhibits the metastasis of gastric cancer by targeting TMEM16A expression. J Exp Clin Cancer Res 2017; 36: 29.
Li B, Xu WW, Han L, Chan KT, Tsao SW, Lee NPY, Law S, Xu LY, Li EM, Chan KW, et al: MicroRNA-377 suppresses initia¬tion and progression of esophageal cancer by inhibiting CD133 and VEGF. Oncogene 2017; 36: 3986-4000.
Rescigno J: Use of postoperative radiotherapy for node-positive non-small-cell lung cancer. Clin Lung Cancer 2002; 4: 35-44.
Brett JO, Renault VM, Rafalski VA, Webb AE and Brunet A: The microRNA cluster miR-106b~25 regulates adult neural stem/proge¬nitor cell proliferation and neuronal differentiation. Aging (Albany NY) 2011; 3: 108-124.
Faraonio R, Salerno P, Passaro F, Sedia C, Iaccio A, Bellelli R, Nappi TC, Comegna M, Romano S, Salvatore G, et al: A set of miRNAs participates in the cellular senescence program in human diploid fibroblasts. Cell Death Differ 2012; 19: 713-721.
Ma DH, Li BS, Liu JJ, Xiao YF, Yong X, Wang SM, Wu YY, Zhu HB, Wang DX and Yang SM: miR-93-5p/IFNAR1 axis promotes gastric cancer metastasis through activating the STAT3 signaling pathway. Cancer Lett 2017; 408: 23-32.
Xiao ZG, Deng ZS, Zhang YD, Zhang Y and Huang ZC: Clinical significance of microRNA-93 downregulation in human colon can¬cer. Eur J Gastroenterol Hepatol 2013; 25: 296-301.
Wang X, Liao Z, Bai Z, He Y, Duan J and Wei L: MiR-93-5p Promotes Cell Proliferation through Down-Regulating PPARGC1A in Hepatocellular Carcinoma Cells by Bioinformatics Analysis and Experimental Verification. Genes (Basel) 2018; 9(1): 51.
Yang W, Bai J, Liu D, Wang S, Zhao N, Che R and Zhang H: MiR-93-5p up-regulation is involved in non-small cell lung cancer cells proliferation and migration and poor prognosis. Gene 2018; 647: 13-20.
Li G, Ren S, Su Z, Liu C, Deng T, Huang D, Tian Y, Qiu Y and Liu Y: Increased expression of miR-93 is associated with poor pro¬gnosis in head and neck squamous cell carcinoma. 2015; Tumour Biol 36: 3949-3956.

References

van der Horst S, Weijs TJ, Ruurda JP, Haj Mohammad N, Mook S, Brosens LAA and van Hillegersberg R: Robot-assisted minimally invasive thoraco-laparoscopic esophagectomy for esophageal can¬cer in the upper mediastinum. J Thorac Dis 2017; 9: S834-s842.

Paireder M, Asari R, Kristo I, Rieder E, Tamandl D, Ba-Ssalamah A and Schoppmann SF: Impact of sarcopenia on outcome in patients with esophageal resection following neoadjuvant chemotherapy for esophageal cancer. Eur J Surg Oncol 2017; 43: 478-484.

Liang Y, Lin Q, Huang P, Wang Y, Li J, Zhang L and Cao J: Rice Bioactive Peptide Binding with TLR4 To Overcome H2O2-Induced Injury in Human Umbilical Vein Endothelial Cells through NF-ÎºB Signaling. J Agri Food Chem 66(2): 440-448, 2018.

Wang L, Lin Q, Yang T, Liang Y, Nie Y, Luo Y and Luo F: Oryza¬nol modifies high fat diet-induced obesity, liver gene expression pro¬file, and inflammation response in mice. J Agri Food Chem 65(38): 8374-8385, 2017.

Lou Y, Shi J, Guo D, Qureshi AK and Song L: Function of PD-L1 in antitumor immunity of glioma cells. Saudi J Boil Sci 24(4): 803-807, 2017.

Guo T, Lin Q, Li X, Nie Y, Wang L, Shi L and Luo F: Octacosa¬nol attenuates inflammation in both RAW264. 7 macrophages and a mouse model of colitis. J Agri Food Chem 65(18): 3647-3658, 2017.

Li W, Jia MX, Wang JH, Lu JL, Deng J, Tang JX and Liu C: As¬sociation of MMP9-1562C/T and MMP13-77A/G polymorphisms with non-small cell lung cancer in southern Chinese population. Biomol 9(3): 107-119, 2019.

Lv Y, Zhang J and Qiao L: Quality of life in patients with esopha¬geal cancer receiving definitive chemoradiotherapy or esophagec¬tomy. Mol Clin Oncol 2014; 2: 870-874.

Nie Y, Luo F, Wang L, Yang T, Shi L, Li X, Shen J, Xu W, Guo T and Lin Q: Anti-hyperlipidemic effect of rice bran polysaccharide and its potential mechanism in high-fat diet mice. Food Func 8(11): 4028-4041, 2017.

Lou Y, Yang J, Wang L, Chen X, Xin X and Liu Y: The clini¬cal efficacy study of treatment to Chiari malformation type I with syringomyelia under the minimally invasive surgery of resection of Submeningeal cerebellar Tonsillar Herniation and reconstruction of Cisterna magna. Saudi J Biol Sci 26(8): 1927-1931, 2019.

Lou Y, Guo D, Zhang H and Song L: Effectiveness of mesenchy¬mal stems cells cultured by hanging drop vs. conventional culturing on the repair of hypoxic-ischemic-damaged mouse brains, measured by stemness gene expression. Open Life Sci 11(1): 519-523, 2016.

Chen X, Xu Y, Meng L, Chen X, Yuan L, Cai Q, Shi W and Huang G: Non-parametric partial least squares–discriminant analy-sis model based on sum of ranking difference algorithm for tea grade identification using electronic tongue data identify tea grade using e-tongue data. Sens Actuators B Chem 127924, 2020.

Yamamoto J, Hayashi T, Izumisawa Y, Kimura J, Takagawa R, Kosaka R, Ono H, Makino H, Tsuburaya A, Akiyama H, et al: [Clini¬cal Experience of Nutritional Support in Patients Treated with Che¬moradiotherapy for Locally Advanced Esophageal Cancer]. Gan To Kagaku Ryoho 2015; 42: 1246-1248.

Lloyd S and Chang BW: Current strategies in chemoradiation for esophageal cancer. J Gastrointest Oncol 2014; 5: 156-165.

Nie Y, Luo F and Lin Q: Dietary nutrition and gut microflora: A promising target for treating diseases. Trends Food Sci Technol 75: 72-80, 2018.

Ren Y, Jiao X and Zhang L: Expression level of fibroblast growth factor 5 (FGF5) in the peripheral blood of primary hypertension and its clinical significance. Saudi J Biol Sci 25(3): 469-473, 2018.

Hui AB, Lenarduzzi M, Krushel T, Waldron L, Pintilie M, Shi W, Perez-Ordonez B, Jurisica I, O'Sullivan B, Waldron J, et al: Com¬prehensive MicroRNA profiling for head and neck squamous cell carcinomas. Clin Cancer Res 2010; 16: 1129-1139.

Igarashi H, Kurihara H, Mitsuhashi K, Ito M, Okuda H, Kanno S, Naito T, Yoshii S, Takahashi H, Kusumi T, et al: Association of MicroRNA-31-5p with Clinical Efficacy of Anti-EGFR Therapy in Patients with Metastatic Colorectal Cancer. Ann Surg Oncol 2015; 22: 2640-2648.

Winther M, Alsner J, Tramm T, Baeksgaard L, Holtved E and Nordsmark M: Evaluation of miR-21 and miR-375 as prognostic biomarkers in esophageal cancer. Acta Oncol 2015; 54: 1582-1591.

Ohta K, Hoshino H, Wang J, Ono S, Iida Y, Hata K, Huang SK, Colquhoun S and Hoon DS: MicroRNA-93 activates c-Met/PI3K/ Akt pathway activity in hepatocellular carcinoma by directly inhibi¬ting PTEN and CDKN1A. Oncotarget 2015; 6: 3211-3224.

Qu MH, Han C, Srivastava AK, Cui T, Zou N, Gao ZQ and Wang QE: miR-93 promotes TGF-beta-induced epithelial-to-mesenchy¬mal transition through downregulation of NEDD4L in lung cancer cells. Tumour Biol 2016; 37: 5645-5651.

Tang Q, Zou Z, Zou C, Zhang Q, Huang R, Guan X, Li Q, Han Z, Wang D, Wei H, et al: MicroRNA-93 suppress colorectal cancer de¬velopment via Wnt/beta-catenin pathway downregulating. Tumour Biol 2015; 36: 1701-1710.

Deng W and Lin SH: Advances in radiotherapy for esophageal cancer. Ann Transl Med 6: 79, 2018

Cao Q, Liu F, Ji K, Liu N, He Y, Zhang W and Wang L: Mi¬croRNA-381 inhibits the metastasis of gastric cancer by targeting TMEM16A expression. J Exp Clin Cancer Res 2017; 36: 29.

Li B, Xu WW, Han L, Chan KT, Tsao SW, Lee NPY, Law S, Xu LY, Li EM, Chan KW, et al: MicroRNA-377 suppresses initia¬tion and progression of esophageal cancer by inhibiting CD133 and VEGF. Oncogene 2017; 36: 3986-4000.

Rescigno J: Use of postoperative radiotherapy for node-positive non-small-cell lung cancer. Clin Lung Cancer 2002; 4: 35-44.

Brett JO, Renault VM, Rafalski VA, Webb AE and Brunet A: The microRNA cluster miR-106b~25 regulates adult neural stem/proge¬nitor cell proliferation and neuronal differentiation. Aging (Albany NY) 2011; 3: 108-124.

Faraonio R, Salerno P, Passaro F, Sedia C, Iaccio A, Bellelli R, Nappi TC, Comegna M, Romano S, Salvatore G, et al: A set of miRNAs participates in the cellular senescence program in human diploid fibroblasts. Cell Death Differ 2012; 19: 713-721.

Ma DH, Li BS, Liu JJ, Xiao YF, Yong X, Wang SM, Wu YY, Zhu HB, Wang DX and Yang SM: miR-93-5p/IFNAR1 axis promotes gastric cancer metastasis through activating the STAT3 signaling pathway. Cancer Lett 2017; 408: 23-32.

Xiao ZG, Deng ZS, Zhang YD, Zhang Y and Huang ZC: Clinical significance of microRNA-93 downregulation in human colon can¬cer. Eur J Gastroenterol Hepatol 2013; 25: 296-301.

Wang X, Liao Z, Bai Z, He Y, Duan J and Wei L: MiR-93-5p Promotes Cell Proliferation through Down-Regulating PPARGC1A in Hepatocellular Carcinoma Cells by Bioinformatics Analysis and Experimental Verification. Genes (Basel) 2018; 9(1): 51.

Yang W, Bai J, Liu D, Wang S, Zhao N, Che R and Zhang H: MiR-93-5p up-regulation is involved in non-small cell lung cancer cells proliferation and migration and poor prognosis. Gene 2018; 647: 13-20.

Li G, Ren S, Su Z, Liu C, Deng T, Huang D, Tian Y, Qiu Y and Liu Y: Increased expression of miR-93 is associated with poor pro¬gnosis in head and neck squamous cell carcinoma. 2015; Tumour Biol 36: 3949-3956.

Author biographies is not available.