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Vol. 66 No. 7: Issue 7

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Optimization of fermentation of Fomes fomentarius extracellular polysaccharide and antioxidation of derivatized polysaccharides

https://doi.org/10.14715/cmb/2020.66.7.10
Qiang Zhang
School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China School of Biological Sciences and Engineering, North Minzu University, Yinchuan 750021,China
Yue Li
School of Biological Sciences and Engineering, North Minzu University, Yinchuan 750021,China
Shuai Zong
School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China
Ming Ye
School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China

Corresponding Author(s) : Qiang Zhang

newman5372@yandex.com

Cellular and Molecular Biology, Vol. 66 No. 7: Issue 7

Abstract

Fermentation is a metabolic process that converts sugars into acids, gases, or alcohol. This process occurs in yeasts and bacteria, as well as in muscle cells when faced with a lack of oxygen. In this paper, isolation, culture, purification and extracellular polysaccharides of strain Fomes fomentarius were studied. Extraction of polysaccharides from a culture based on F. fomentarius extracellular polysaccharides, extracellular polysaccharides fermentation experiments was optimized and compared, the optimal fermentation method was obtained; extracellular polysaccharides were sulfated, phosphorylated experiments, selenium acidified, discussed the preparation of derivative polysaccharides and microscopic detection, and finally studied extracellular polysaccharides on DPPH, The scavenging ability, superoxide anion radical and hydroxyl radical scavenging ability of the derived polysaccharides were compared. The results showed that the extracellular polysaccharide and derivatized polysaccharide of F. fomentarius had certain antioxidant activity.

Keywords

Fomes fomentarius Response surface optimization Antioxidation Derivatized polysaccharide.
Zhang, Q., Li, Y., Zong, S., & Ye, M. (2020). Optimization of fermentation of Fomes fomentarius extracellular polysaccharide and antioxidation of derivatized polysaccharides. Cellular and Molecular Biology, 66(7), 56–65. https://doi.org/10.14715/cmb/2020.66.7.10
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References
  1. Wei C, Zhao Z, Shi-Fei C, Yong-Quan L. Optimization for the production of exopolysaccharide from Fomes fomentarius in submerged culture and its antitumor effect in vitro. Biores Technol 2007; 99(8) :3187-3194.
  2. Lee JS. Effects of Fomes fomentarius supplementation on antioxidant enzyme activities, blood glucose, and lipid profile in streptozotocin-induced diabetic rats. Nutr Res 2005; 25(2):187-195.
  3. Jaszek M, Żuchowski J, Dajczak E, Cimek K, Gra̢z M, Grzywnowicz K. Ligninolytic enzymes can participate in a multiple response system to oxidative stress in white-rot basidiomycetes: Fomes fomentarius and Tyromyces pubescens. Int Biodeterior Biodegradation 2006; 58(3-4):168-75.
  4. Liu L, Zhou SB, Zheng WF. Antitumor activity of ethanol extract from P. sinensis and its effect on immune function of tumor-bearing mice. J Xuzhou Medical College 2007; 27(8): 497-499.
  5. Lu XY. Determination of Polysaccharide in Cordycextracellular polysaccharide militaris L. by Sulfuric Acid-Phenol Method. Sci Technol Food Indust 2002; 23(4): 69-70
  6. Xu ZL, Zhang S. Isolation, Purification and Content Determination of Polysaccharides in Cordycextracellular polysaccharide sinensis Fermentation Broth. J Zhejiang University Trad Chin Med 2007; 31(3): 376-377.
  7. Wu J. Principles and Techniques of Biochemistry Experiments. Baoding: Hebei University Press, 2005:22-29.
  8. Li Jianwu. Principles and Methods of Biochemistry Experiments. Beijing: Peking University Press, 1994:36-46; 207-216.
  9. Olberg S, Andersen J. Field attraction of beetles (Coleoptera) to the polypores Fomes fomentarius and Phellinus spp (Fungi: Aphyllophorales) in northern Norway. Entomol Generalis. 2000; 1:217-36.
  10. Chen W, Zhao Z, Li Y. Simultaneous increase of mycelial biomass and intracellular polysaccharide from Fomes fomentarius and its biological function of gastric cancer intervention. Carbohydrate Polymers. 2011; 85(2):369-75.
  11. Yu J, Jiang Y, Wang S. Biochemical Experimental Technology.Beijing: Chemical Industry Publishing House, 2005:138-139.
  12. Zhao Y, Ji L, Zhu X. Extraction, component analysis and preliminary structural identification of fungal polysaccharides. Food Sci 2005; 26 (9): 127-129.
  13. Chen H, Li YH, Yao QX. Application of ion exchange technology in separation and purification of polysaccharides. J Hebei Agric Sci 2008; 12(7): 168-169.
  14. Zang Y, Xiong J, Zhai WZ, Cao L, Zhang SP, Tang Y, Wang J, Su JJ, Yang GX, Zhao Y, Fan H. Fomentarols A–D, sterols from the polypore macrofungus Fomes fomentarius. Phytochem 2013; 92:137-45.
  15. Judova J, Dubikova K, Gaperova S, Gaper J, Pristas P. The occurrence and rapid discrimination of Fomes fomentarius genotypes by ITS-RFLP analysis. Fungal Biol 2012; 116(1):155-60.
  16. Neifar M, Jaouani A, Ellouze-Ghorbel R, Ellouze-Chaabouni S. Purification, characterization and decolourization ability of Fomes fomentarius laccase produced in solid medium. J Mol Catalysis B: Enzymatic. 2010; 64(1-2):68-74.
  17. Zhao JY, Ding JH, Li ZH, Dong ZJ, Feng T, Zhang HB, Liu JK. Three new phenyl-ethanediols from the fruiting bodies of the mushroom Fomes fomentarius. J Asian Nat Prod Res 2013; 15(3):310-4.
  18. Thunes KH, Willassen E. Species composition of beetles (Coleoptera) in the bracket fungi Piptoporus betulinus and Fomes fomentarius (Aphyllophorales: Polyporaceae): an explorative approach with canonical correspondence analysis. J Nat Hist1997; 31(3): 471-486.
  19. Gáper J, Gáperová S, Pristas P, Naplavova K. Medicinal value and taxonomy of the tinder polypore, Fomes fomentarius (Agaricomycetes): a review. Int J Med Mushrooms. 2016;18(10).
  20. Prasad L, Kundu A, Bahukhandi D. Comparative analysis of volatile fractions of Fomes fomentarius and F. rhabarbarinus. Indian Phytopathol 2018; 71(1):25-31.
  21. Judova J, Dubikova K, Gaperova S, Gaper J, Pristas P. The occurrence and rapid discrimination of Fomes fomentarius genotypes by ITS-RFLP analysis. Fung Biol 2012; 116(1):155-60.
  22. Chen W, Zhao Z, Chen SF, Li YQ. Optimization for the production of exopolysaccharide from Fomes fomentarius in submerged culture and its antitumor effect in vitro. Biores Technol 2008; 99(8): 3187-94.
  23. He GY, Lei LS, Chen J, Yu CL, Ren J, Chen NN. Process optimization for extraction of immune active polysaccharides from Fomes fomentarius by introduction of ultrasonication. Zhong yao cai= Zhongyaocai= J Chin Med Mat 2011; 34(8):1277-80.
  24. Prendecka M, Jaszek M, Grć…z M, GÅ‚uszak N, MaÅ‚ysz K, Nowak A, Å»uchowski J, MaÅ‚ecka"Massalska T. Stimulation of the activity of a novel tannase produced in white"rot fungi Phellinus pini, Fomes fomentarius, and Tyromyces pubescens by medium supplementation. Biotechnol Appl Biochem 2016; 63(5): 652-8.
  25. Chen C, Kang C, Rong N, Wu N, Chen C, Wu S, Zhang X, Liu X. Evaluation of Immunogenicity, Protective Immunity on Aquaculture Pathogenic Vibrio and Fermentation of Vibrio alginolyticus Flagellin FlaC Protein. Iran J Biotechnol. 2019; 17(3): e2628.
  26. Baharara J, Amini E, Musavi M. Anti-Vasculogenic Activity of a Polysaccharide Derived from Brittle Star via Inhibition of VEGF, Paxillin and MMP-9. Iran J Biotechnol. 2017; 15(3): 179-185.
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