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Vol. 66 No. 7: Issue 7

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Effects of dendrobium polysaccharides on human brain microvascular endothelial cell injury induced by ox-LDL via regulating the miR-378 expression

https://doi.org/10.14715/cmb/2020.66.7.11
Lai Chen
Neurology Department, Shanghai TCM-Integrated Hospital Affiliated to Shanghai University of TCM, Shanghai, 200082, China
Shaohui Zhang
Rehabilitation Department, Shanghai Jiangwan Hospital, Shanghai, 200434, China
Xiaoying Liu
Neurology Department, Shanghai TCM-Integrated Hospital Affiliated to Shanghai University of TCM, Shanghai, 200082, China
Jiong Dai
Neurosurgery Department, Renji Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, 200127, China
Zhenguo Yan
Neurology Department, Shanghai TCM-Integrated Hospital Affiliated to Shanghai University of TCM, Shanghai, 200082, China
Chunlan Wu
Neurology Department, Shanghai TCM-Integrated Hospital Affiliated to Shanghai University of TCM, Shanghai, 200082, China

Corresponding Author(s) : Jiong Dai

zg18836@163.com

Cellular and Molecular Biology, Vol. 66 No. 7: Issue 7

Abstract

Brain microvascular endothelial cells are a key part of the blood-brain barrier. This experiment was set up to investigate the effect and molecular mechanism of Dendrobium polysaccharide on oxidized low-density lipoprotein (ox-LDL)-induced damage to the human brain microvascular endothelial cells. For this purpose, human brain microvascular endothelial cells HBMEC were divided into control group (without any treatment), ox-LDL group (50 μg/mL ox-LDL), Dendrobium polysaccharide low, medium and high concentration group (0.1 μg/L, 0.2 μg/L, 0.4 μg/L Dendrobium polysaccharide+50 μg/mL ox-LDL), ox-LDL+miR-NC group (transfection miR-378 mimic negative control+50 μg/mL ox-LDL), ox-LDL+miR-378 group (transfected miR-378 mimics+50 μg/mL ox-LDL), ox-LDL+DP+anti-miR-NC group (transfected miR-378 inhibitor negative control +0.4 μg/L Dendrobium polysaccharide+50 μg/mL ox-LDL), ox-LDL+DP+anti-miR-378 group (transfected miR-378 inhibitor+0.4 μg/L Dendrobium polysaccharide+50 μg/mL ox-LDL ). The kit was used to detect the levels of malondialdehyde (MDA) and the activities of superoxide dismutase (SOD) and catalase (CAT); flow cytometry to detect apoptosis; and Western blot to detect B-cell lymph tumor/leukemia-2 (Bcl-2) and Bcl-2 related X (Bax) protein expression; real-time fluorescence quantitative PCR (RT-qPCR) was used to detect the expression of miR-378. Results showed that after treatment with different concentrations of Dendrobium polysaccharides, MDA levels were decreased in ox-LDL-induced human brain microvascular endothelial cells, SOD and CAT activities were increased, apoptosis rate was decreased, Bcl-2 expression was increased, Bax expression was decreased, miR-378 expression was increased, in a dose-dependent manner (P<0.05). Overexpression of miR-378 inhibits ox-LDL-induced oxidative stress and apoptosis in human brain microvascular endothelial cells. Inhibition of miR-378 expression reversed the effect of Dendrobium polysaccharide on ox-LDL-induced damage to human brain microvascular endothelial cells. Then dendrobium polysaccharide may inhibit ox-LDL-induced oxidative stress and apoptosis in human brain microvascular endothelial cells by up-regulating the expression of miR-378.

Keywords

Dendrobium polysaccharide miR-378 ox-LDL human brain microvascular endothelial cells oxidative damage
Chen, L., Zhang, S., Liu, X., Dai, J., Yan, Z., & Wu, C. (2020). Effects of dendrobium polysaccharides on human brain microvascular endothelial cell injury induced by ox-LDL via regulating the miR-378 expression. Cellular and Molecular Biology, 66(7), 66–71. https://doi.org/10.14715/cmb/2020.66.7.11
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