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Phytochemical screening of Alstonia venenata leaf and bark extracts and their antimicrobial activities
Corresponding Author(s) : Ryszard Amarowicz
amaro@pan.olsztyn.pl
Cellular and Molecular Biology,
Vol. 66 No. 4: Medicinal plants and natural products
Abstract
Alstonia venenata is a plant commonly found in South India and used in traditional medicine. The aim of this study was to characterize the phytochemicals present in A. venenata leaf and bark extracts and study their antimicrobial activities. Solvent extractions with Soxhlet apparatus of leaves and bark were obtained using hexane, benzene, isopropanol, methanol, and water. The crude extracts were concentrated and screened for qualitative phytochemical content and analyzed by thin layer chromatography. The antibacterial, antifungal and antiviral activities of crude extracts were measured by in vitro methods. Alkaloids, carbohydrates, tannins, phenolic compounds, terpenoids, cardiac glycosides and amino acids were found in the different crude extracts analyzed. Isopropanol extracts showed antifungal activity and it was more pronounced in the bark extract than the leaf extract. Moreover, the isopropanol extract exhibited antibacterial and antiviral activity. In conclusion, the leaves and bark of A. venenata have antimicrobial components which are more present in the isopropanol fraction.
Keywords
Alstonia venenata
Phytochemical
Antimicrobial
Antibacterial
Antifungus.
Bagheri, G., Ayatollahi, S. A., Ramírez-Alarcón, K., Salehi, B., Mohammadi, R., Rahmani, A., Martorell, M., Sharifi-Rad, J., Sheibani, M., & Amarowicz, R. (2020). Phytochemical screening of Alstonia venenata leaf and bark extracts and their antimicrobial activities. Cellular and Molecular Biology, 66(4), 224–231. https://doi.org/10.14715/cmb/2020.66.4.27
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