miR-409-3p inhibits the proliferation and migration of human ovarian cancer cells by targeting Rab10

Yanyi Li; Li Chen; Beibei Zhang; Yuko Ohno; Hua Hu

doi:10.14715/cmb/2020.66.7.30

Issue

Vol. 66 No. 7: Issue 7

Issue Published : November 9, 2020

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miR-409-3p inhibits the proliferation and migration of human ovarian cancer cells by targeting Rab10

https://doi.org/10.14715/cmb/2020.66.7.30

Yanyi Li

Department of Health Science, Graduate School of Medical, Osaka University, Osaka 565-0871, Japan

Li Chen

Department of Gynecology and Obstetrics, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai 201399, P.R. China

Beibei Zhang

Department of Gynecology and Obstetrics, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai 201399, P.R. China

Yuko Ohno

Department of Health Science, Graduate School of Medical, Osaka University, Osaka 565-0871, Japan

Hua Hu

Department of Gynecology and Obstetrics, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai 201399, P.R. China

Corresponding Author(s) : Yuko Ohno

xxgzx4@163.com

Cellular and Molecular Biology, Vol. 66 No. 7: Issue 7
Article Published : October 31, 2020

Abstract

Ovarian cancer is a leading cause of gynecological cancer-related mortality. It has been reported that miR-409-3p is involved in the proliferation and migration of cancer cells. However, the role of miR-409-3p in ovarian cancer has not been well studied. The present study aimed to investigate the functional role of miR-409-3p in the pathogenesis of ovarian cancer, and its potential mechanism. It was found that the expression levels of miR-409-3p in 6 ovarian cancer tissues were upregulated. Through proliferation, migration and colony formation assays, it was revealed that overexpression of miR-409-3p inhibited the proliferation and migration of ovarian cancer cells. It was predicted from bioinformatics assays that the complementary binding sites were within miR-409-3p and Rab10. It was also demonstrated that the downregulation of the expression of Rab10 reversed the miR-409-3p downregulation-induced abnormal proliferation of ovarian cancer cells. These results suggest that miR-409-3p expression can be used as a predictive marker for the prognosis of ovarian cancer. Thus, the miR-409-3p/Rab10 axis may be a novel therapeutic target for ovarian cancer.

Keywords

Ovarian cancer miR-409-3p Rab10.

Li, Y., Chen, L., Zhang, B., Ohno, Y., & Hu, H. (2020). miR-409-3p inhibits the proliferation and migration of human ovarian cancer cells by targeting Rab10. Cellular and Molecular Biology, 66(7), 197–201. https://doi.org/10.14715/cmb/2020.66.7.30

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References

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Torre LA, Trabert B, DeSantis CE, Miller KD, Samimi G, Runowicz CD, et al. Ovarian cancer statistics, 2018. CA Cancer J Clin 2018; 68: 284-296.
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Bai R, Weng C, Dong H, Li S, Chen G, Xu Z. MicroRNA-409-3p suppresses colorectal cancer invasion and metastasis partly by targeting GAB1 expression. Int J Cancer 2015; 137: 2310-2322.
Josson S, Gururajan M, Hu P, Shao C, Chu GY, Zhau HE, et al. miR-409-3p/-5p promotes tumorigenesis, epithelial-to-mesenchymal transition, and bone metastasis of human prostate cancer. Clin Cancer Res 2014; 20: 4636-4646.
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Ridge PG, Karch CM, Hsu S, Arano I, Teerlink CC, Ebbert MTW, et al. Alzheimer's Disease Neuroimaging, Linkage, whole genome sequence, and biological data implicate variants in RAB10 in Alzheimer's disease resilience. Genome Med 2017; 9: 100.
Wang Y, Liu P, Qiu L, Sun Y, Zhu M, Gu L, et al. Toxicity and therapy of cisplatin-loaded EGF modified mPEG-PLGA-PLL nanoparticles for SKOV3 cancer in mice. Biomaterials 2013; 34: 4068-4077.
Zhou Z, Huang F. Long Non-Coding RNA LINC00152 Regulates Cell Proliferation, Migration And Invasion In Esophageal Squamous Cell Carcinoma Via miR-107/Rab10 Axis. Onco Targets Ther 2019; 12: 8553-8567.
Zhang X, Wang S, Lin G, Wang D. Down-regulation of circ-PTN suppresses cell proliferation, invasion and glycolysis in glioma by regulating miR-432-5p/RAB10 axis, Neurosci Lett 2020; 735: 135153.
Chen MW, Yang ST, Chien MH, Hua KT, Wu CJ, Hsiao SM, et al. The STAT3-miRNA-92-Wnt Signaling Pathway Regulates Spheroid Formation and Malignant Progression in Ovarian Cancer. Cancer Res 2017; 77: 1955-1967.
Wang W, Jia WD, Hu B, Pan YY. RAB10 overexpression promotes tumor growth and indicates poor prognosis of hepatocellular carcinoma. Oncotarget 2017; 8: 26434-26447.
Wauters F, Cornelissen T, Imberechts D, Martin S, Koentjoro B, Sue C, et al. LRRK2 mutations impair depolarization-induced mitophagy through inhibition of mitochondrial accumulation of RAB10. Autophagy 2020; 16: 203-222.
Jiang W, Liu J, Xu T, Yu X. MiR-329 suppresses osteosarcoma development by downregulating Rab10. FEBS Lett 2016; 590: 2973-2981.

References

Matulonis UA, Sood AK, Fallowfield L, Howitt BE, Sehouli J, Karlan BY, et al. Nat Rev Dis Primers 2016; 2: 16061.

Torre LA, Trabert B, DeSantis CE, Miller KD, Samimi G, Runowicz CD, et al. Ovarian cancer statistics, 2018. CA Cancer J Clin 2018; 68: 284-296.

Ali AT. Towards Prevention of Ovarian Cancer. Curr Cancer Drug Targets 2018; 18: 522-537.

Meng L, Liu C, Lü J, Zhao Q, Deng S, Wang G, et al. Small RNA zippers lock miRNA molecules and block miRNA function in mammalian cells. Nat Commun 2017; 8: 13964.

Xu J, Shao T, Ding N, Li Y, Li X. miRNA-miRNA crosstalk: from genomics to phenomics. Brief Bioinform 2017; 18: 1002-1011.

Shao T, Wang G, Chen H, Xie Y, Jin X, Bai J, et al. Survey of miRNA-miRNA cooperative regulation principles across cancer types. Brief Bioinform 2019; 20: 1621-1638.

Weng C, Dong H, Chen G, Zhai Y, Bai R, Hu H, et al. miR-409-3p inhibits HT1080 cell proliferation, vascularization and metastasis by targeting angiogenin. Cancer Lett 2012; 323: 171-179.

Liu T, Zhong S, Rao F, Xue Y, Qi Z, Wu S. Catheter ablation restores decreased plasma miR-409-3p and miR-432 in atrial fibrillation patients. Europace 2016; 18: 92-99.

Bai R, Weng C, Dong H, Li S, Chen G, Xu Z. MicroRNA-409-3p suppresses colorectal cancer invasion and metastasis partly by targeting GAB1 expression. Int J Cancer 2015; 137: 2310-2322.

Josson S, Gururajan M, Hu P, Shao C, Chu GY, Zhau HE, et al. miR-409-3p/-5p promotes tumorigenesis, epithelial-to-mesenchymal transition, and bone metastasis of human prostate cancer. Clin Cancer Res 2014; 20: 4636-4646.

English AR, Voeltz GK. Rab10 GTPase regulates ER dynamics and morphology. Nat Cell Biol 2013; 15: 169-178.

Ridge PG, Karch CM, Hsu S, Arano I, Teerlink CC, Ebbert MTW, et al. Alzheimer's Disease Neuroimaging, Linkage, whole genome sequence, and biological data implicate variants in RAB10 in Alzheimer's disease resilience. Genome Med 2017; 9: 100.

Wang Y, Liu P, Qiu L, Sun Y, Zhu M, Gu L, et al. Toxicity and therapy of cisplatin-loaded EGF modified mPEG-PLGA-PLL nanoparticles for SKOV3 cancer in mice. Biomaterials 2013; 34: 4068-4077.

Zhou Z, Huang F. Long Non-Coding RNA LINC00152 Regulates Cell Proliferation, Migration And Invasion In Esophageal Squamous Cell Carcinoma Via miR-107/Rab10 Axis. Onco Targets Ther 2019; 12: 8553-8567.

Zhang X, Wang S, Lin G, Wang D. Down-regulation of circ-PTN suppresses cell proliferation, invasion and glycolysis in glioma by regulating miR-432-5p/RAB10 axis, Neurosci Lett 2020; 735: 135153.

Chen MW, Yang ST, Chien MH, Hua KT, Wu CJ, Hsiao SM, et al. The STAT3-miRNA-92-Wnt Signaling Pathway Regulates Spheroid Formation and Malignant Progression in Ovarian Cancer. Cancer Res 2017; 77: 1955-1967.

Wang W, Jia WD, Hu B, Pan YY. RAB10 overexpression promotes tumor growth and indicates poor prognosis of hepatocellular carcinoma. Oncotarget 2017; 8: 26434-26447.

Wauters F, Cornelissen T, Imberechts D, Martin S, Koentjoro B, Sue C, et al. LRRK2 mutations impair depolarization-induced mitophagy through inhibition of mitochondrial accumulation of RAB10. Autophagy 2020; 16: 203-222.

Jiang W, Liu J, Xu T, Yu X. MiR-329 suppresses osteosarcoma development by downregulating Rab10. FEBS Lett 2016; 590: 2973-2981.

Author biographies is not available.