Issue

Vol. 66 No. 8: Issue 8

The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.

Effect of paeonol on proliferation, apoptosis, migration, invasion and glutamine of gastric cancer cells via circSFMBT2/miR-665 axis

https://doi.org/10.14715/cmb/2020.66.8.6
Jia Li
Endoscope Room, Dalian Port Hospital, Dalian, 116001, China
Gang Zhang
Endoscope Room, Dalian Port Hospital, Dalian, 116001, China
Guoqiang Wu
Endoscope Room, Dalian Port Hospital, Dalian, 116001, China

Corresponding Author(s) : Gang Zhang

gangzhang0207@outlook.com

Cellular and Molecular Biology, Vol. 66 No. 8: Issue 8

Abstract

This experiment was performed to investigate the effect of paeonol on the proliferation, apoptosis, migration, invasion and glutamine of gastric cancer HGC-27 cells and its possible mechanism. For this purpose, the MTT method was used to detect cell viability; Flow cytometry experiment was used to detect cell apoptosis; Transwell chamber experiment was used to detect cell migration and invasion; Western blotting was used to detect the expression levels of MMP2 and MMP9 protein; The decomposition of glutamine was evaluated by detecting the expression levels of glutamine, glutamic acid and α-ketoglutarate (α-KG). This study used RT-PCR to detect the expression of circSFMBT2 and miR-665. The targeting relationship between circSFMBT2 and miR-665 was verified by the dual-luciferase report experiment and RIP experiment. Results showed that different concentrations of Paeonol could significantly inhibit the proliferation, migration, invasion and glutamine decomposition of HGC-27 cells, and induce cell apoptosis in a dose-dependent manner. In gastric cancer tissues and cells, the expression of circSFMBT2 was up-regulated, and the expression of miR-665 was down-regulated. Over-expression of circSFMBT2 could partially restore the effects of paeonol on the proliferation, apoptosis, migration, invasion and glutamine of HGC-27 cells. CircSFMBT2 could target and negatively regulate the expression of miR-665. Overexpression of miR-665 could partially restore the effects of Pae and circSFMBT2 on the proliferation, apoptosis, migration, invasion and glutamine of HGC-27 cells. It was concluded that paeonol can inhibit the proliferation, migration, invasion and glutamine decomposition of gastric cancer HGC-27 cells via circSFMBT2/miR-665 axis, and also induce cell apoptosis.

Keywords

Paeonol Gastric cancer Proliferation Apoptosis Migration Invasion Glutamine.
Li, J., Zhang, G., & Wu, G. (2020). Effect of paeonol on proliferation, apoptosis, migration, invasion and glutamine of gastric cancer cells via circSFMBT2/miR-665 axis. Cellular and Molecular Biology, 66(8), 33–40. https://doi.org/10.14715/cmb/2020.66.8.6
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. Choi YJ, Kim N. Gastric cancer and family history. Korean J Intern Med 2016; 31(6): 1042-1053.
  2. Coccolini F, Nardi M, Montori G, et al. Neoadjuvant chemotherapy in advanced gastric and esophago-gastric cancer. Meta-analysis of randomized trials. Int J Surg 2018; 51: 120-127.
  3. Tan Z. Recent advances in the surgical treatment of advanced gastric cancer: A review. Med Sci Monit 2019; 25: 3537-3541.
  4. Zhang L, Li DC, Liu LF. Paeonol: pharmacological effects and mechanisms of action. Int Immunopharmacol 2019; 72: 413-421.
  5. Lyu ZK, Li CL, Jin Y, Liu YZ, Zhang X, Zhang F, Ning LN, Liang ES, Ma M, Gao W, Zhang MX, Liu DS. Paeonol exerts potential activities to inhibit the growth, migration and invasion of human gastric cancer BGC823 cells via downregulating MMP"‘2 and MMP"‘9. Mol Med Rep 2017; 16(5):7513-7519.
  6. Li BJ, Jin MM, Cao FF, et al. Hsa_circ_0017639 expression promotes gastric cancer proliferation and metastasis by sponging miR-224-5p and upregulating USP3. Gene 2020; 750: 144753.
  7. Wu KZ, Zhang CD, Zhang C, et al. miR-665 suppresses the epithelial-mesenchymal transition and progression of gastric cancer by targeting CRIM1. Cancer Manag Res 2020; 12: 3489-3501.
  8. Adki KM, Kulkarni YA. Chemistry, pharmacokinetics, pharmacology and recent novel drug delivery systems of paeonol. Life Sci 2020; 250:117544. doi: 10.1016/j.lfs.2020.117544. Epub 2020 Mar 13. PMID: 32179072.
  9. Lei Y, Li HX, Jin WS, Peng WR, Zhang CJ, Bu LJ, Du YY, Ma T, Sun GP. The radiosensitizing effect of Paeonol on lung adenocarcinoma by augmentation of radiation-induced apoptosis and inhibition of the PI3K/Akt pathway. Int J Radiat Biol 2013; 89(12):1079-86.
  10. Gao L, Wang Z, Lu D, Huang J, Liu J, Hong L. Paeonol induces cytoprotective autophagy via blocking the Akt/mTOR pathway in ovarian cancer cells. Cell Death Dis 2019;10(8): 609.
  11. Bordbar M, Darvishzadeh R, Pazhouhandeh M, Kahrizi D. An overview of genome editing methods based on endonucleases. Mod Genet J 2020; 15(2): 75-92.
  12. Fu J, Yu LH, Luo J, et al. Paeonol induces the apoptosis of the SGC"‘7901 gastric cancer cell line by downregulating ERBB2 and inhibiting the NF"‘κB signaling pathway. Int J Mol Med 2018; 42(3): 1473-1483.
  13. Fang X, Wen J, Sun M, Yuan Y, Xu Q. CircRNAs and its relationship with gastric cancer. J Cancer. 2019; 10(24): 6105-6113.
  14. Li R, Jiang JJ, Shi H, et al. CircRNA: a rising star in gastric cancer. Cell Mol Life Sci 2020; 77(9): 1661-1680.
  15. Shen FQ, Liu PJ, Xu ZQ, et al. CircRNA_001569 promotes cell proliferation through absorbing miR-145 in gastric cancer. J Biochem, 2019; 165(1): 27-36.
  16. Liang M, Huang GQ, Liu ZY, et al. Elevated levels of hsa_circ_006100 in gastric cancer promote cell growth and metastasis via miR-195/GPRC5A signalling. Cell Prolif 2019; 52(5): e12661.
  17. Mi LL, Lei LH, Yin XL, et al. Circ_0000144 functions as a miR-623 sponge to enhance gastric cancer progression via up-regulating GPRC5A. Biosci Rep 2020; 40(8): BSR20201313.
  18. Fan JH, Li HP, Nie X, et al. MiR-665 aggravates heart failure via suppressing CD34-mediated coronary microvessel angiogenesis. Aging (Albany NY) 2018; 10(9): 2459-2479.
  19. Dong CH, Du QY, Wang ZM, et al. MicroRNA-665 suppressed the invasion and metastasis of osteosarcoma by directly inhibiting RAB23. Am J Transl Res 2016; 8(11): 4975-4981.
  20. Zhang MJ, Wang S, Yi AW, et al. microRNA-665 is down-regulated in gastric cancer and inhibits proliferation, invasion, and EMT by targeting PPP2R2A. Cell Biochem Funct 2020; 38(4): 409-418.
  21. association between gastric cancer and hopq alleles in Helicobacter pylori. Genetika 2016; 48(3): 893-902 Kazemi E, Kahrizi D. Lack of
  22. Kazemi E, Kahrizi D, Moradi MT, Sohrabi M, Yari K. Gastric cancer and Helicobacter pylori: impact of hopQII gene. Cell Mol Biol 2016; 62(2): 107-110.
  23. Kazemi E, Kahrizi D, Moradi MT, Sohrabi M, Amini A, Mousavi SAR, Yari K. Association between Helicobacter pylori hopQI genotyping and human gastric cancer. Cell Mol Biol 2016; 62(1): 6-9.
  24. Kazemi E, Kahrizi D, Moradi MT, Sohrabi, M, Amini S, Mousavi S.A.R., Yari K. Association between Manganese Superoxide Dismutase (MnSOD Val-9Ala) genotypes with the risk of generalized aggressive periodontitis disease. Cell Mol Biol 2016; 61 (8): 49-52.
  25. Zhang J, Liu B. A review on the recent developments of sequence-based protein feature extraction methods. Curr Bioinform 2019;14(3):190-9. Doi: 10.2174/1574893614666181212102749.
  26. Xu L, Jiang S, Zou Q. An in silico approach to identification, categorization and prediction of nucleic acid binding proteins. bioRxiv. 2020. Doi: 10.1093/bib/ bbaa171.
  27. Zhu S, Wang X, Zheng Z, Zhao XE, Bai Y, Liu H. Synchronous measuring of triptolide changes in rat brain and blood and its application to a comparative pharmacokinetic study in normal and Alzheimer's disease rats. Journal of Pharmaceutical and Biomedical Analysis. 2020; 113263. 10.1016/j.jpba.2020.113263.
  28. Chen G, Li Y, Ren Z, Gu Y, Tang F, Mao J, Zhu J, Wang L, Li Y. Clinical Significance of MicroRNA-155-Regulated Autophagy and Apoptosis by Targeting Rictor/Fos in Gastric Cancer Progression. Nanosci Nanotechnol Lett 2020; 12(4):525-35.
  29. Alkhudhayri AA, Wahab R, Siddiqui MA, Ahmad J. Selenium Nanoparticles Induce Cytotoxicity and Apoptosis in Human Breast Cancer (MCF-7) and Liver (HepG2) Cell Lines. Nanosci Nanotechnol Lett 2020;12(3):324-30.
  30. Jin Y, Zhu H, Zhu H, Jin F, Shi C, Yang L, Qian J, Zhang S. Fluorouracil Nanoliposomes Promote Apoptosis of Human Gastric Cancer Xenografts in Nude Mice. Nanosci. Nanotechnol. Lett 2020; 12: 690–695.
  31. Lin J, Wang Y, Wei X, Kong S, Liu Z, Liu J, Zhang F, Lin S, Ji B, Zhou Z, Guo Z. Controllable antibacterial and bacterially anti-adhesive surface fabricated by a bio-inspired beetle-like macromolecule. Int J Biol Macromol 2020. Doi: 10.1016/j.ijbiomac.2020.04.207.
  32. Zhang D, Lu Z, Sun B. Highly Sensitive Gold Nanoparticle Polymerase Chain Reaction in the Detection of Anaplastic Lymphoma Kinase-Positive Gastric Cancer from a Biopsy Specimen. Nanosci Nanotechnol Lett 2020; 12(4):498-505.
  33. Wu Y, Liu C, Gao M, Liang Q, Jiang Y. Effect of Titanium Nanoparticles on Osteoblast Proliferation. Nanosci Nanotechnol Lett 2020;12(4):455-60.
  34. Bai J, Guo T, Dong W, Song Y, Guo T, Cui M. Reduction of Breast Cancer Lymph Node Metastasis by Nano-Carbon Absorption of 5-Fluorouracil. Nanoscience and Nanotechnology Letters. 2020 Mar 1;12(3):407-12.
  35. Zhang T, Su H, Xing Y, Zhang J, Xu D. Protective Mechanism of Lipid-Lowering Ketone and Self-Assembled OA Chitosan Nanoparticles on Insulin Oxidative Stress. Induced by High Fat in BRL-3A Cells. Nanosci Nanotechnol Lett 2020; 12: 715–719.
  36. Kazemi E, Zargooshi J Kaboudi M, Heidari P, Kahrizi D, Mahaki B, Mohammadian Y, Khazaei H, Ahmed K. A genome-wide association study to identify candidate genes for erectile dysfunction. Brief Bioinform 2020; bbaa338, https://doi.org/10.1093/bib/bbaa338.
Read More
Author biographies is not available.
Crossref
Scopus
Google Scholar
Europe PMC
Download this PDF file
PDF
Statistic
Read Counter : 1014 Download : 511