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Copyright (c) 2022 Ihsan K. A. Alkardhi, Hatem Masmoudi , Hayder A. Muhammedand, Hayet Sellami
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.Epidemiological Study with Evaluation of Molecular and Immunologicalassay for Detection of Toxoplasma gondii in aborted women
Corresponding Author(s) : Ihsan K. A. Alkardhi
Cellular and Molecular Biology,
Vol. 68 No. 4: Issue 4
Abstract
Toxoplasmosis, caused by Toxoplasma gondii, is one of the most prevalent parasite illnesses in humans. Although primary infection in a pregnant woman is usually asymptomatic, it has the potential to cause significant harm to the fetus, including miscarriage. In this study, we investigate the usefulness of the PCR to confirm the etiology of the abortion. A prospective study in the Al-Diwaniyah maternity and pediatric teaching hospital in Iraq was conducted. The research comprised 94 aborted women. We have reported a new internal primer for the nested PCR protocol to detect toxoplasmosis. In the 94-aborted women, 30 samples (31.9 %) were positive by the nPCR using the G529 repeat gene and qPCR using B1 gene primers. We have shown that three women carry the parasite in their placentas, and at the same time, they do not carry antibodies in their blood.
We recommend that women should be aware of the risk of toxoplasmosis and the importance of preventing measures. In addition, PCR should be done in the case of abortion to enhance sensitivity even if serology is negative.
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