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Copyright (c) 2022 Hua'an Zhang, Wanhao Ye, Yingpu Li
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.The effect of MiR-497 on the expression of target genes BCL-2 and LC3B on cardiomyocytes injured by hypoxia/reoxygenation
Corresponding Author(s) : Yingpu Li
Cellular and Molecular Biology,
Vol. 68 No. 5: Issue 5
Abstract
Myocardial ischemia is easy to cause hypoxia or necrosis of myocardial cells. At present, the performance of various patients is different. Basically, it is mainly caused by chest pain or chest discomfort. Severe patients may die suddenly. Therefore, looking for effective drugs or methods to prevent and treat Cardiomyocyte injury is of great significance for clinical practice, in which the expression of regulatory gene BCL-2 and microtubule-associated protein light chain 3B (LC3B) has a certain effect on hypoxia/reoxygenation injured cardiomyocytes. To this end, the team designed a study on the effect of miR-497 on the expression of target genes BCL-2 and LC3B on cardiomyocytes injured by hypoxia/reoxygenation. In this study, a control group experiment was set up for the study. During the experiment, the cells were treated with hypoxia-reoxygenation and transfected with the corresponding miR-497 treated cells. By detecting apoptosis, the kit was used to detect cell activity and RT-PCR detection. Gene expression levels and other methods are comparatively judged. The results of this study showed that compared with the normal group 14.50±0.78, the viability of cardiomyocytes in the model group was significantly reduced (P<0.01), the amount of NO released by cardiomyocytes was reduced (P<0.01), and the protein expression in cardiomyocytes was significantly reduced (P<0.01). The experimental results of this study prove that miR-497 can alleviate the damage caused by hypoxia-reoxygenation of cardiomyocytes by regulating target genes BCL-2 and LC3B.
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