Issue
Copyright (c) 2022 Liuzhong Wu, Danyang Shen, Chuanbo Guo
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.Effects of recombinant human growth hormone on proliferation and differentiation of cementoblast and ERK1 / 2, JNK / SAPK and p38MAPK signaling pathway
Corresponding Author(s) : Chuanbo Guo
Cellular and Molecular Biology,
Vol. 68 No. 7: Issue 7
Abstract
To investigate the effects of recombinant human growth hormone on the proliferation and differentiation of cementoblast and the signal pathways of ERK1 / 2, JNK / SAPK and p38MAPK, osteoblasts (OCCM-30) were cultured in vitro. The OCCM-30 was treated with different concentrations of recombinant human growth hormone (rhGH) (0, 10, 50 ng/mL) for 1 d and 2 d, respectively. MTT assay was used to test the proliferation of OCCM-30 cells by rhGH. The effect of BSP, OPN, OCN and ALP genes was detected by RT-PCR after one day. The activity of alkaline phosphatase (ALP) was detected after treatment with OCCM-30 for five days at different concentrations of rhGH. After treatment with OCCM-30 at 100 ng/mL rhGH for 0 min, 5 min, 10 min, 15 min, 30 min and 60 min, the phosphorylation levels of ERK1/2, JNK/SAPK and p38MAPK were detected by Western blot. Results showed that rhGH could promote the proliferation of OCCM-30 cells, and the proliferation of OCCM-30 cells increases with the increase of rhGH concentration. After one day of culture, the levels of the BSP and ALP genes increased with the increase of rhGH concentration (P<0.05); the OPN gene level in the 10 ng/mL group was significantly higher than that in the blank group, and the 50 ng/mL group was significantly lower than the blank group. (P<0.05); OCN gene levels in the 10 ng/mL group and 50 ng/mL group were not significantly different from those in the blank group (P>0.05). Compared with the blank group, the ERK 1/2 phosphorylation level increased at 5 min in the 100 ng/mL group, reached the maximum at 10 min, decreased significantly at 15 min, decreased to the original level at 30 min, and had no significant change in ERK 1/2 total protein level; 100 ng/mL rhGH had no significant effect on SAPK/JNK, p38MAPK phosphorylation and total protein levels in OCCM-30 cells (P>0.05). It was concluded that 10ng/mL and 50ng/mL rhGH could promote the proliferation of OCCM-30 cells and promote the expression of the BSP gene and ALP gene. Low-dose rhGH is beneficial to OPN gene expression, and high-dose rhGH inhibits OPN gene expression. 100 ng/mL rhGH promoted the ERK 1/2 pathway and had no effect on the SAPK/JNK and p38 MAPK pathways.
Keywords
Download Citation
Endnote/Zotero/Mendeley (RIS)BibTeX
