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Copyright (c) 2022 Namama Soran Al-Talabani, Payman Ghoraishizadeh, Shwan Rachid
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.Mastering noninvasive predictive biomarkers to follow up renal transplant patients
Corresponding Author(s) : Shwan Rachid
Cellular and Molecular Biology,
Vol. 68 No. 8: Issue 8
Abstract
Renal transplantation is the treatment of choice for end-stage renal disease (ESRD) patients. Several cellular processes are regulated via non-coding RNAs by silencing target gene expression. Previous investigations have established a linkage between a number of human microRNAs and kidney failure. This study aims to identify the expression of urinary miR-199a-3p and miR-155-5p as non-invasive biomarkers during post and pre-transplantation over a six-month follow-up period. In addition to the classic chronic renal disease markers (estimated glomerular filtration rate eGFR, Serum creatinine, serum electrolytes, and Antinuclear antibodies ANA test). Urinary miR-199a-3p and miR-155-5p expression levels in 72 adults with diabetic nephropathy and, 42 adults with lupus nephropathy renal transplant recipients. Both were compared with 32 healthy controls prior and post-transplantation. miRNAs were evaluated by quantitative reverse transcription-polymerase chain reaction. Urinary miR-199a-3p significantly (p<0.0001) downregulated in diabetic and lupus nephropathy prior to transplantation and significantly upregulated post-transplantation compared to the control. While urinary miR-155-5p quantities were significantly higher in prior renal transplant patients in comparison with the same patients’ post-renal transplantation(P<0.0001). In conclusion, Urinary miR-199a-3p and miR-155-5p can be used as a non-invasive biomarker with high specificity and sensitivity to follow up the renal transplant patients before and post-transplantation instead of biopsy which is complicated by a non-negligible factor.
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