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Copyright (c) 2022 Lifang Zhao
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.Exploration of Values of MiR-7110-5p and MiR-223-3p in Predicting Sepsis
Corresponding Author(s) : Lifang Zhao
Cellular and Molecular Biology,
Vol. 68 No. 8: Issue 8
Abstract
The study aimed to explore the values of micro ribonucleic acid (miR)-7110-5p and miR-223-3p in predicting sepsis secondary to pneumonia. MiRNAmicroarray was utilized to analyze the expression difference of miRNA in patients with pneumonia and sepsis secondary to pneumonia. A total of 50 patients with pneumonia and 42 patients with sepsis secondary to pneumonia were included. Then quantitative polymerase chain reaction (qPCR) was carried out to detect the expression level of circulating miRNAs in patients and analyze its correlations with clinical characteristics and prognosis. 9 miRNAs, namely, hsa-miR-4689-5p, hsa-miR-4621-5p, hsa-miR-6740-5p, hsa-miR-7110-5p, hsa-miR-765, hsa-miR-940,hsa-miR-213-5p, hsa-miR-223-3p and hsa-miR-122, met the screening criteria of fold change ≥2 or <0.5 and p<0.01. The expression levels of miR-4689-5p and miR-4621-3p were different between the two groups of patients, which were up-regulated in the plasma of patients with sepsis secondary to pneumonia. The expression levels of miR-7110-5p and miR-223-3p in patients with pneumonia and sepsis were higher than those in healthy controls. Besides, the area under curve (AUC) of the receiver operating characteristic (ROC) curve of miR-7110-5p for predicting pneumonia and sepsis secondary to pneumonia was 0.78 and 0.863, respectively, while that of miR-223-3p for predicting pneumonia and sepsis secondary to pneumonia was 0.879 and 0.924, respectively. However, there were no significant differences in the levels of miR-7110-5p and miR-223-3p in plasm between survived and dead patients with sepsis. MiR-7110-5p and miR-223-3p can serve as potential biological indicators for predicting sepsis secondary to pneumonia.
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