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Copyright (c) 2022 Omer Faruq Yousif, Sawsan M. Sorchee
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.Molecular Identification of Yersinia enterocolitica Isolated from Different Sources in Erbil City
Corresponding Author(s) : Omer Faruq Yousif
Cellular and Molecular Biology,
Vol. 68 No. 8: Issue 8
Abstract
Yersinia enterocolitica ranks third among the pathogens that often cause the digestive disorder. It is transmitted to humans through food materials, especially contaminated meats. This research aimed to survey the frequency of Yersinia enterocolitica in sheep local products, especially meat, in Erbil. To carry out this study, 500 samples of raw milk, soft cheese, ice cream, and meat were collected by random sampling from different shops in Erbil City of Iraq. The samples were divided into four groups, including raw milk, soft cheese, ice cream, and meat. Many microbiological tests (culture, & staining, biochemical Tests, Vitek 2, and species-specific Polymerase Chain Reaction (PCR) amplicon for 16S RNA gene) were performed. Results showed that 5.1% of all of the studied samples were contaminated with Yersinia enterocolitica. Analysis of the results showed that meat is more contaminated than other samples. Also, the sequenced DNA evolutionary phylogeny tree of Yersinia enterocolitica isolates showed that all bacteria originated from the same genus and species. Therefore, it is recommended to pay special attention to this issue in order to avoid health and economic risks.
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