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Copyright (c) 2022 Hua Xiang, Longqing Liu, Yuzhu Yuan, Linghui Liu, Zhiwei Chen
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.Inhibition Mechanism of PinX1 Gene on Cancer Stem Cells of Nasopharyngeal Carcinoma
Corresponding Author(s) : Zhiwei Chen
Cellular and Molecular Biology,
Vol. 68 No. 10: Issue 10
Abstract
The recurrence and long-term metastasis of these tumors are important causes of treatment failure and death. On the other hand, PinX1 is a nucleolar protein found in recent years that can interact with telomere/telomerase simultaneously, and it is highly conserved in human and yeast. Some studies have shown that the PinX1 gene can inhibit the tumor stem cells of NPC. Therefore, the mechanism of inhibition of the PinX1 gene on the tumor stem cells of NPC has been studied in this paper. In this paper, CNE2 cells of NPC were used as experimental materials, CD133 as a marker, PinX1 overexpression plasmids and their corresponding empty plasmids were respectively transfected in CD133+ cells, PinX1 siRNA and their corresponding NC siRNA were respectively transfected in CD133- cells for control experiments. In this study, we found that the telomerase activity of the CD133 - + NC group was 1.001 ± 0.086, the CD133 - + pinx1sirna group was 0.974 ± 0.046, CD133+ + vector group was 0.928 ± 0.102, CD133+ + over PinX1 group was 0.703 ± 0.086. Therefore, the PinX1 gene can inhibit NPC stem cells by inhibiting telomerase activity.
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