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Copyright (c) 2023 Saya Hadi Raouf Raouf, Diyar Khalid Bakr Bakr, Urfa Muneer Ahmed Ahmed, Bassam Karem Amin Amin
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.In vitro evaluation of the antibacterial effects of MTA- Fillapex and BIO-C® sealer at different time intervals
Corresponding Author(s) : Saya Hadi Raouf
Cellular and Molecular Biology,
Vol. 69 No. 4: Issue 4
Abstract
Failure of endodontic treatment is closely associated with bacterial infection, the study aimed to evaluate the antibacterial properties of MTA- Fillapex and BIO-C against two bacterial species Enterococcus faecalis (E. faecalis) and Staphylococcus aureus (S. aureus). In this in vitro study, two endodontic sealers were used to evaluate antibacterial properties by using an agar diffusion test (ADT) and direct contact test (DCT). The width of the growth inhibition zone after 24 hours was reported in (ADT) as the endodontic sealers' effectiveness. In DCT, the survival of microorganisms was assessed at various periods following the exposure of the bacterial suspension to the sealers for (20 and 40 min) at (1 day, 7 days and 14 days). Colony-forming unit (CFU) counts were made. In ADT, the zones of microbial growth inhibition in BIO-C sealer caused by E. Facealis were relatively larger than that created due to S. Auerous bacteria with mean 0.781 mm and 0.538 mm respectively. Thus, this difference turned out to be significant (p<0.001). In DCT At both time points tested, no significant difference was found among control cases against the tested microbes (p > 0.05). In terms of sealers, BIO-C had the most potent antimicrobial properties. It showed significant inhibition against both E.faecalis and S. Auerous on day 1 and the first week of contact times tested. Moreover both BIO-C and MTA Fillapex sealers perform worthy antibacterial activity up to 1 week and BIO-C sealers show better antibacterial efficacy against E. faecalis than MTA Fillapex sealers.
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