Shashi Prabha  Singh; Vandana  Maurya; Krishan  Kumar; Durgesh  Singh; Poonam Verma; Dipan  Samanta; Manish Kumar  Verma

doi:10.14715/cmb/2023.69.6.6

Issue

Vol. 69 No. 6: Issue 6

Issue Published : August 17, 2023

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Serum Expression Level of MicroRNA-122 and its Significance in different Stages of Hepatitis B Virus Infection

MicroRNA-122 and its effects on Hepatitis B

https://doi.org/10.14715/cmb/2023.69.6.6

Shashi Prabha Singh

Department of Biochemistry, Maa Vindhyavasini Autonomous State Medical College Mirzapur, India

Vandana Maurya

Department of Obstetrics and gynecology, Maa Vindhyavasini Autonomous State Medical College Mirzapur, India

Krishan Kumar

Department of Medicine, United Institute of Medical Sciences, Allahabad, India

Durgesh Singh

Department of Anatomy, Maa Vindhyavasini Autonomous State Medical College Mirzapur, India

Poonam Verma

Department of Biotechnology, IFTM University, Moradabad, India

Dipan Samanta

Department of Gynecology and Obstetrics, Dali University, Dali City, PR China

Manish Kumar Verma

Department of Biochemistry, Rajarshi Dashrath Autonomous State Medical College, Ayodhya, India

Corresponding Author(s) : Durgesh Singh

Dr.dpsingh84@gmail.com

Cellular and Molecular Biology, Vol. 69 No. 6: Issue 6
Article Published : June 30, 2023

Abstract

This study aims to determine the serum expression level of miRNA-122 and its significance in the different stages of Hepatitis B virus infection. The study subjects were recruited and grouped for Hepatitis B associated with Chronic Hepatitis B infection, hepatic sclerosis, hepatocellular carcinoma, and healthy controls were also considered. Venous blood was collected from the participants including the controls and routine blood tests and quantification of miRNA-122 were done and analyzed in each case of hepatitis B infection and compared with that of healthy controls. The miRNA-122 was determined, which came to be highest in patients with Chronic Hepatitis B while patients with hepatic sclerosis and patients with hepatocellular carcinoma showed a subsequent number of copies. The number of copies of miRNA-122 in the CHB, hepatic sclerosis, and HCC group was significantly higher than in the healthy control. The quantification of miRNA-122 and subsequently plotting the ROC curve has shown that miRNA-122 can be considered as a biomarker of hepatitis B for screening and diagnosis purposes.

Keywords

Biomarker, Hepatitis B, Liver, miRNA, RNA

Singh, S. P. ., Maurya, V. ., Kumar, K. ., Singh, D. ., Verma, P., Samanta, D. ., & Verma, M. K. . (2023). Serum Expression Level of MicroRNA-122 and its Significance in different Stages of Hepatitis B Virus Infection: MicroRNA-122 and its effects on Hepatitis B. Cellular and Molecular Biology, 69(6), 36–40. https://doi.org/10.14715/cmb/2023.69.6.6