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Copyright (c) 2023 Joong Hyun Shim
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.Anti-aging Effect of Allium pseudojaponicum in UVA–irradiated Human Epidermal Keratinocytes
Corresponding Author(s) : Joong Hyun Shim
Cellular and Molecular Biology,
Vol. 69 No. 8: Issue 8
Abstract
This study was conducted to investigate the anti-aging effects of Allium pseudojaponicum extract on normal human epidermal keratinocytes (NHEKs). The effects were examined by analyzing gene expressions related to skin hydration using quantitative real-time polymerase chain reaction (qRT-PCR), hyaluronic acid (HA) production using HA-ELISA, cell viability using a cell viability assay, and a phospho-kinase array. Allium pseudojaponicum extract increased the gene expression levels of AQP3/HAS2 and HA protein production in NHEKs while decreasing the overexpressed mRNA levels of KRT1, 10 and FLG genes, known as differentiated keratinocyte markers in NHEKs. Additionally, A. pseudojaponicum extract reduced the phosphorylation of CHK2 and p53 proteins, which are related to cell cycle or epidermal differentiation. This study demonstrated the anti-aging effects of A. pseudojaponicum extract, which could potentially be used as a functional ingredient for skin hydration and anti-aging products.
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