Elevated PD-1 expression and decreased telomerase activity in memory T cells of patients with symptomatic Herpes Zoster infection

Z. Zangeneh; H. Golmoghaddam; M. Emad; N. Erfani; M. Doroudchi

Issue

Vol. 60 No. 4: Issues 4

Issue Published : December 31, 2014

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Elevated PD-1 expression and decreased telomerase activity in memory T cells of patients with symptomatic Herpes Zoster infection

Z. Zangeneh

H. Golmoghaddam

M. Emad

N. Erfani

M. Doroudchi

Corresponding Author(s) : M. Doroudchi

mdoroud@sums.ac.ir

Cellular and Molecular Biology, Vol. 60 No. 4: Issues 4
Article Published : November 15, 2014

Abstract

We investigated PD-1 levels on VZV-specific CD8+ T-cells of patients with zoster and the effect of PD-1 on the telomerase activity. CD3, CD8, CD137 and PD-1 expressions were analyzed on PBMCs from 9 symptomatic and 5 asymptomatic individuals. The effect of PD-1 blockade at the time of stimulation on the telomerase activity of non-senescent CD57-CD45RO+CD8+CD3+ memory T-cells was evaluated. PD-1 was elevated on CD8+ T-cells in patients. The frequency of PD-1+ and CD137- cells in total CD3+CD8+ T cells of patients was elevated compared to controls. Telomerase activity of non-senescent memory T-cells was lower than that of controls. Blockade of PD-1 at the time of stimulation increased telomerase activity of non-senescent memory T-cells, accompanied by increased CD137 expression. Low telomerase activity of the patients with reactivated zoster could be partially overcome by blocking PD-1 pathway.

Keywords

Memory PD-1 T cell Telomerase.

Zangeneh, Z., Golmoghaddam, H., Emad, M., Erfani, N., & Doroudchi, M. (2014). Elevated PD-1 expression and decreased telomerase activity in memory T cells of patients with symptomatic Herpes Zoster infection. Cellular and Molecular Biology, 60(4), 13–21. Retrieved from https://mail.cellmolbiol.org/index.php/CMB/article/view/514