Issue
The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.
RNAi-mediated silencing of ATP-binding cassette C4 protein inhibits cell growth in MGC80-3 gastric cancer cell lines
Corresponding Author(s) : W. Zhang
wenjiezhangdr@163.com
Cellular and Molecular Biology,
Vol. 60 No. 1: Issues 1
Abstract
ATP-binding cassette C4/multidrug resistance associated protein 4 (ABCC4/MRP4) is a relatively well-studied member of the ATP-binding cassette drug transporter family. Recent studies suggested that ABCC4 is implicated in the development of several human cancers. In the present study, we report that knockdown of ABCC4 by lentivirus-delivered short hairpin RNA could inhibit gastric cancer cell growth in vitro. The proliferation of MGC80-3 cells was significantly reduced after infection with Lv-shABCC4, as determined by MTT and colony formation assays. In addition, knockdown of ABCC4 blocked the cell cycle progression in MGC80-3 cells. To our knowledge, it is the first report to investigate the role of ABCC4 in gastric tumorigenesis. Our data provides additional evidence that ABCC4/MRP4 could be a potential therapeutic target for gastric cancers.
Keywords
ABCC4
gastric cancer
shRNA
proliferation.
Chen, L., Gu, J., Xu, L., Qu, C., Zhang, Y., & Zhang, W. (2014). RNAi-mediated silencing of ATP-binding cassette C4 protein inhibits cell growth in MGC80-3 gastric cancer cell lines. Cellular and Molecular Biology, 60(1), 1–5. Retrieved from https://mail.cellmolbiol.org/index.php/CMB/article/view/536
Download Citation
Endnote/Zotero/Mendeley (RIS)BibTeX