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Copyright (c) 2025 Jianfeng Liao, Zili Yi, Qian Sun, Yulai Han
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.ESTs identification and construction of normalized cDNA libraries of Miscanthus lutarioriparius across combinations of salt and drought stresses
Corresponding Author(s) : Qian Sun
Cellular and Molecular Biology,
Vol. 71 No. 5: Issue 5
Abstract
Miscanthus lutarioriparius is a perennial C4 herb with high biomass production and is widely utilized as a non-food biobased material for bioproduction. This study successfully constructed two high-quality full-length normalized cDNA libraries from distinct salt-tolerant accessions of M. lutarioriparius under salt, drought, and combined salt-drought stress conditions. The study identified 420 high-quality Expressed Sequence Tags (ESTs) primarily associated with signal transduction mechanisms, post-translational modifications, energy production and transformation, as well as the synthesis, transport, and metabolism of amino acids, carbohydrates, and secondary metabolites. A total of 1370 Gene Ontology (GO) terms were obtained from two accessions, mainly related to cellular process, metabolic process, response to stimulus, biological regulation, biological regulation, cellular anatomical entity, binding, and catalytic activity. Five GO terms from the Biological Process Ontology consistently exhibited high P-values in both accessions, primarily associated with responses to exogenous substances and metabolic processes. The significant enrichment of genes associated with cellular components such as the chloroplast matrix, cytoplasm, and plastid matrix from the Cellular Component Ontology may explain the salt-drought tolerance mechanism of Miscanthus. This study is expected to deepen our understanding of the functional genes in Miscanthus plants and may provide a reference for screening salt and drought-resistance genes.
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