Molecular and agro-morphological genetic diversity assessment of Chickpea mutants induced via ethyl methane sulfonate

Abstract

Iran, especially its western provinces, is one of the most chickpea producing countries of the world with the yield about 500 kg/ ha in average. Narrow genetic variability for chickpea is one of the most limitations in conventional breeding approaches. In this study, derived genetic variation among 94 chickpea (Bivanij cultivar) mutant lines produced by Ethyl Methane Sulfonate (EMS) were assessed based on ISSR, RAPD markers in M4 and morpho-agronomic traits in M3 generation. The induced variation via EMS in field experiment, showed significant differences among mutant lines based on almost measured traits. In overall, banding patterns of 6 ISSR primers and 8 RAPD primers revealed 21 (50%) and 24 (42.25%) polymorphic bands, respectively. The ranges of similarity coefficient in ISSR and RAPD markers were 0.62-1.00 and 0.72-1.00, respectively. Specific grouping was carried out by each cluster analysis including ISSR, RAPD, ISSR+RAPD and morpho-agronomic markers based on their similarity matrices. The results showed significant variation generated by EMS based on molecular markers and morpho-agronomic traits. Mantel tests between extracted similarity matrices from each marker system were statistically significant. It could be concluded that the generated variation with EMS as a chemical mutant can be used for chickpea breeding purposes.