Issue
The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.
microRNA-33A expression is reduced in cerebral cortex in a rat model of ischemic tolerance
Corresponding Author(s) : Y Feng
fengyanfy1028@163.com
Cellular and Molecular Biology,
Vol. 61 No. 3: Issue 3
Abstract
This study examined the microRNA-33a (miR-33a) expression levels in cerebral cortex in rat model of cerebral ischemic tolerance (IT), with 3-nitropropionic acid (3-NPA) preconditioning. Rat model of cerebral IT was established as follows: 14 male Sprague-Dawley rats were randomly divided into two groups, the 3-NPA treated group and the control group. Intraperitoneal injection of 3-NPA or normal saline was performed in 3-NPA treated group and control group, respectively. Middle cerebral artery occlusion (MCAO) was performed in all the rats 3 days after injection and the infarct volume was measured on postoperative day-2. To study miR-33a expression levels in this model, male Sprague-Dawley rats (n = 31) were randomly divided into 4 groups, 3-NPA treatment group (n = 8), 3-NPA treatment + MCAO group (n = 9), MCAO group (n = 9), and control group (n = 5). Surviving rats were sacrificed 4 days after injection and cerebral cortex samples were obtained for total RNA isolation. MiR-33a expression levels were determined by real-time quantitative PCR (qRT-PCR). Infarct volume in 3-NPA treated group decreased significantly in comparison to the control group (P < 0.05). Expression analysis by qRT-PCR revealed that miR-33a expression levels in 3-NPA treated group were significantly higher than that in control group (P < 0.05). However, no statistically significant difference in miR-33a expression levels were observed in 3-NPA + MCAO and MCAO groups (both P > 0.05). Our results present convincing evidence that miR-33a expression levels are significantly reduced in the cerebral cortex in rat model of cerebral IT, which may have significantly impacted the infarct volume.
Keywords
MicroRNA-33a
Middle cerebral artery occlusion
Cerebral ischemic tolerance
3-nitropropionic acid
Animal model
Real-time quantitative RT-PCR
Infarct volume
Cerebral cortex.
Feng, Y., Li, W., & Wang, J. Q. (2015). microRNA-33A expression is reduced in cerebral cortex in a rat model of ischemic tolerance. Cellular and Molecular Biology, 61(3), 24–29. Retrieved from https://mail.cellmolbiol.org/index.php/CMB/article/view/664
Download Citation
Endnote/Zotero/Mendeley (RIS)BibTeX