Antibacterial activities of essential oils from Iranian medicinal plants on extended-spectrum Î²-lactamase-producing Escherichia coli

J Sharifi-Rad; D Mnayer; A Roointan; F Shahri; S A M Ayatollahi; M Sharifi-Rad; N Molaee; M Sharifi-Rad

Issue

Vol. 62 No. 9: Natural products as antimicrobial agents from molecular approach

Issue Published : August 29, 2016

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Antibacterial activities of essential oils from Iranian medicinal plants on extended-spectrum Î²-lactamase-producing Escherichia coli

J Sharifi-Rad

Zabol University of Medical Sciences Zabol Medicinal Plants Research Center Zabol Iran

D Mnayer

Lebanese University Faculty of Agricultural Engineering and Veterinary Medicine Dekwaneh Lebanon

A Roointan

Shiraz university of Medical sciences Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies Shiraz Iran

F Shahri

Zahedan University of Medical Sciences Department of Optometry, School of Rehabilitation Zahedan Iran

S A M Ayatollahi

Shahid Beheshti University of Medical Sciences Phytochemistry Research Center Tehran Iran

M Sharifi-Rad

University of Zabol Department of Chemistry, Faculty of Science Zabol Iran

N Molaee

Arak University of Medical Sciences Department of Microbiology and Immunology Arak Iran

M Sharifi-Rad

Zabol University of Medical Sciences Zabol Iran

Corresponding Author(s) : J Sharifi-Rad

Cellular and Molecular Biology, Vol. 62 No. 9: Natural products as antimicrobial agents from molecular approach
Article Published :

Abstract

The extended-spectrum beta-lactamase (ESBL) -producing Escherichia coli strains can lead to various infections particularly urinary tract infections. The main objective of this investigation was to evaluate the antibacterial activities of essential oils (EOs) from different Iranian medicinal plants against TEM gene positive ESBL-producing E. coli strains isolated from urine samples of patients with urinary tract infections. EOs were extracted using hydrodistillation method. E. coli strains were isolated by different specific Medias. ESBL-producing E. coli strains were isolated from urine samples of patients with urinary tract infections in Shiraz hospital, Iran. Then, ESBL- producing strains were identified using double disk synergy test, phenotypic disc confirmatory test and polymerase chain reaction (PCR) for TEM gene detection. The antibacterial activity of the EOs from different plants (Achillea wilhelmsii C. Koch, Echinophora platyloba DC., Lallemantia royleana, Nepeta persica Boiss., Pulicaria vulgaris Gaertn., Salvia nemorosa, and Satureja intermedia C.A.Mey) and antibiotics against ESBL-producing strains was studied using the microdilution method for the evaluation of the minimum inhibitory concentration (MIC). The 103 out of 295 E. coli strains with 97 (90.65%) TEM gene distributions were identified as ESBL-producing strains. All of the EOs derived from different plants displayed high inhibitory effects against ESBL-producing E. coli strains. The results of our investigations may propose a good treatment option against resistant infectious bacteria.

Keywords

Essential oils Escherichia coli antibacterial activities.

Sharifi-Rad, J., Mnayer, D., Roointan, A., Shahri, F., Ayatollahi, S. A. M., Sharifi-Rad, M., Molaee, N., & Sharifi-Rad, M. (2016). Antibacterial activities of essential oils from Iranian medicinal plants on extended-spectrum Î²-lactamase-producing Escherichia coli. Cellular and Molecular Biology, 62(9), 75–82. Retrieved from https://mail.cellmolbiol.org/index.php/CMB/article/view/924