Effect of Stevia rebaudiana Bertoni extract on sexual dysfunction in Streptozotocin-induced diabetic male rats

Matin Ghaheri; Shahram Miraghaee; Atefeh Babaei; Bahareh Mohammadi; Danial Kahrizi; Zahra Minoosh Saivosh Haghighi; Gholamreza Bahrami

doi:10.14715/cmb/2018.64.2.2

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Vol. 64 No. 2: New biotechnological aspects in Stevia rebaudiana Bertoni

Issue Published : February 11, 2018

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Effect of Stevia rebaudiana Bertoni extract on sexual dysfunction in Streptozotocin-induced diabetic male rats

https://doi.org/10.14715/cmb/2018.64.2.2

Matin Ghaheri

Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

Shahram Miraghaee

Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

Atefeh Babaei

Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

Bahareh Mohammadi

Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

Danial Kahrizi

Department of Agronomy and Plant Breeding, Faculty of Agriculture, Razi University, Kermanshah, Iran

Zahra Minoosh Saivosh Haghighi

Faculty of Veterinary Medicine of Razi universiry. Kermanshah, Iran

Gholamreza Bahrami

Department of Agronomy and Plant Breeding, Faculty of Agriculture, Razi University, Kermanshah, Iran

Corresponding Author(s) : Atefeh Babaei

atefeh_babaie@yahoo.com

Cellular and Molecular Biology, Vol. 64 No. 2: New biotechnological aspects in Stevia rebaudiana Bertoni
Article Published : February 10, 2018

Abstract

Stevia rebaudiana Bertoni has been used locally as a non-calorie sweetener in medicine and diabetic diet which claimed to have aphrodisiac properties, although no scientific data of this function have been reported. The aim of this study was to investigate the effect of S. rebaudiana extract on sexual dysfunction, testosterone levels and number of Leydig cells in Streptozotocin (STZ)-induced diabetic male rats. A total of 28 diabetic male rats were randomly divided into 4 groups: diabetic group without any extract and 3 extract groups (5, 50 and 100 mg/kg). Seven normal control rats were treated with vehicle mount latency and frequency of (ML, MF), intromission latency and frequency (IL, IF), ejaculation latency and frequency of (EL, EF), the mount latency post ejaculation (MPE), the intromission latency post ejaculation (ILE), the intromission frequency post ejaculation (IFE) were recorded during 30 min on days 0, 14, 28. The serum testosterone levels, blood glucose, sex organs weight, number of leydig cells and histology of testicular tissue were measured. The stevia group (5 mg/kg) had a significant (p<0.05) increase in EF and IF. The number of Leydig cells in the diabetic group were significantly (p<0.05) reduced compared to the normal group and diabetic groups with extract (5 and 50 mg/kg). The serum testosterone levels and other sexual behaviors did not show any significant differences. The low- dose stevia extract with attention to antioxidant, vasodilator and anti-diabetic properties can be aphrodisiac in STZ- induced diabetic male rats.

Keywords

Diabetes Sexual dysfunction Stevia Rebaudiana Bertoni Leydig cells Testosterone levels Male rats.

Ghaheri, M., Miraghaee, S., Babaei, A., Mohammadi, B., Kahrizi, D., Saivosh Haghighi, Z. M., & Bahrami, G. (2018). Effect of Stevia rebaudiana Bertoni extract on sexual dysfunction in Streptozotocin-induced diabetic male rats. Cellular and Molecular Biology, 64(2), 6–10. https://doi.org/10.14715/cmb/2018.64.2.2

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References

Scheen AJ.Pathophysiology of type 2 diabetes. Acta Clin Belg 2003; 58(6): 335-41.
Chen Y, et al.Losartan improves erectile dysfunction in diabetic patients: a clinical trial. Int J Impot Res 2012; 24(6): 217-20.
NIH Consensus Conference. Impotence. NIH Consensus Development Panel on Impotence. Jama1993; 270(1): 83-90.
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Wankeu-Nya M, Watcho P, Nguelefack TB, Carro-Juarez M, Tapondjou L, Kamanyi A. Effects of Dracaena arborea (Dracaenaceae) on sexual dysfunction in 4 weeks hyperglycemic male rats. Asian Pac J Trop Med 2014; 7(8): 609-619.
Lert-Amornpat T, Maketon C, Fungfuang, W. Effect of Kaempferia parviflora on sexual performance in streptozotocin-induced diabetic male rats. Andrologia 2017; 6 pp.
Cellek S, Cameron NE, Cotter MA, Muneer A. Pathophysiology of diabetic erectile dysfunction: potential contribution of vasa nervorum and advanced glycation endproducts. Int J Impot Res 2013; 25(1): 1-6.
Momtazi-Borojeni AA, Esmaeili SA, Abdollahi E, Sahebkar A. A Review on the Pharmacology and Toxicology of Steviol Glycosides Extracted from Stevia rebaudiana. Curr Pharm Des 2017; 23(11): 1616-1622.
Ashwell M. Stevia, Nature's Zero-Calorie Sustainable Sweetener: A New Player in the Fight Against Obesity. Nutr Today 2015; 50(3): 129-134.
Gupta R, Yadav V, Rastogi M. A Review on importance of natural sweetner, a zero calorie plant- Stevia- having medicinal and commercial importance. Int J Food Nutr Sci 2014;3(3): 90-94.
Shahi M, Mohammadyfar MA.Comparison of depression, anxiety, stress, quality of life, and alexithymia between people with type II diabetes and non-diabetic counterparts.Pers Individ Dif 2017; 104: 64-68.
Juan C, Gil PL, Flores C, Pedro Y, Chimoy J.Long-term effects of the consumption of Stevia rebaudiana (Magnoliopsida, Asteraceae) on fertility mice's. Rev peru biol 2008; 15(1): 85-90.
Melis MS.Effects of chronic administration of Stevia rebaudiana on fertility in rats. J Ethnopharmacol 1999; 67(2): 157-161.
Curry LL, Roberts A, Brown N.Rebaudioside A: Two-generation reproductive toxicity study in rats.Food Chem Toxicol 2008; 46(7): 21-30.
Kaliste EE.The Welfare of Laboratory Animals.Springer 2007; 2(ISBN 978-1-4020-2271-5 (e-book).
Scarano WR, Messias AG, Oliva SU, Klinefelter GR, Kempinas WG. Sexual behaviour, sperm quantity and quality after short-term streptozotocin-induced hyperglycaemia in rats. Int J Androl 2006; 29(4): 482-8.
Xie BG, Li J, Zhu WJ.Pathological changes of testicular tissue in normal adult mice: A retrospective analysis.Exp Ther Med 2014; 7(3): 654-656.
Konrad RJ, Mikolaenko I, Tolar JF, Liu K, Kudlow JE. The potential mechanism of the diabetogenic action of streptozotocin: inhibition of pancreatic beta-cell O-GlcNAc-selective N-acetyl-beta-D-glucosaminidase.Biochem J 2001; 356(1): 31-41.
Tamas V, Kempler P.Sexual dysfunction in diabetes. Handb Clin Neurol 2014; 126: 223-32.
Argiolas A.Neuropeptides and sexual behaviour. Neurosci BiobehavRev 1999; 23(8): 1127-42.
Jason Gandhi GD, Warren K, Smith N, Ali Khan S.Effect of Diabetes Mellitus on Sexual Arousal and Intercourse. Transl Biomed 2016; 7: 2.
Melis MS.A crude extract of Stevia rebaudiana increases the renal plasma flow of normal and hypertensive rats. Braz J Med Biol Res 1996; 29(5): 669-75.
Shivanna N, Naika M, Khanum F, Kaul VK. Antioxidant, anti-diabetic and renal protective properties of Stevia rebaudiana. J Diabetes Complications 2013; 27(2): 103-13.
Agarwal A, et al.Role of Oxidative Stress in the Pathophysiological Mechanism of Erectile Dysfunction.J Androl 2006; 27(3): 335-347.
Hurtado de Catalfo GE, De Gómez Dumm LNT.Lipid dismetabolism in Leydig and Sertoli cells isolated from streptozotocin-diabetic rats. Int J Biochem Cell Biol 1998; 30(9): 1001-1010.

References

Scheen AJ.Pathophysiology of type 2 diabetes. Acta Clin Belg 2003; 58(6): 335-41.

Chen Y, et al.Losartan improves erectile dysfunction in diabetic patients: a clinical trial. Int J Impot Res 2012; 24(6): 217-20.

NIH Consensus Conference. Impotence. NIH Consensus Development Panel on Impotence. Jama1993; 270(1): 83-90.

Maiorino MI, Bellastella, G, Esposito K.Diabetes and sexual dysfunction: current perspectives. Diabetes, Metabolic Syndrome and Obesity. Targets and Therapy 2014; 7: 95-105.

Wankeu-Nya M, Watcho P, Nguelefack TB, Carro-Juarez M, Tapondjou L, Kamanyi A. Effects of Dracaena arborea (Dracaenaceae) on sexual dysfunction in 4 weeks hyperglycemic male rats. Asian Pac J Trop Med 2014; 7(8): 609-619.

Lert-Amornpat T, Maketon C, Fungfuang, W. Effect of Kaempferia parviflora on sexual performance in streptozotocin-induced diabetic male rats. Andrologia 2017; 6 pp.

Cellek S, Cameron NE, Cotter MA, Muneer A. Pathophysiology of diabetic erectile dysfunction: potential contribution of vasa nervorum and advanced glycation endproducts. Int J Impot Res 2013; 25(1): 1-6.

Momtazi-Borojeni AA, Esmaeili SA, Abdollahi E, Sahebkar A. A Review on the Pharmacology and Toxicology of Steviol Glycosides Extracted from Stevia rebaudiana. Curr Pharm Des 2017; 23(11): 1616-1622.

Ashwell M. Stevia, Nature's Zero-Calorie Sustainable Sweetener: A New Player in the Fight Against Obesity. Nutr Today 2015; 50(3): 129-134.

Gupta R, Yadav V, Rastogi M. A Review on importance of natural sweetner, a zero calorie plant- Stevia- having medicinal and commercial importance. Int J Food Nutr Sci 2014;3(3): 90-94.

Shahi M, Mohammadyfar MA.Comparison of depression, anxiety, stress, quality of life, and alexithymia between people with type II diabetes and non-diabetic counterparts.Pers Individ Dif 2017; 104: 64-68.

Juan C, Gil PL, Flores C, Pedro Y, Chimoy J.Long-term effects of the consumption of Stevia rebaudiana (Magnoliopsida, Asteraceae) on fertility mice's. Rev peru biol 2008; 15(1): 85-90.

Melis MS.Effects of chronic administration of Stevia rebaudiana on fertility in rats. J Ethnopharmacol 1999; 67(2): 157-161.

Curry LL, Roberts A, Brown N.Rebaudioside A: Two-generation reproductive toxicity study in rats.Food Chem Toxicol 2008; 46(7): 21-30.

Kaliste EE.The Welfare of Laboratory Animals.Springer 2007; 2(ISBN 978-1-4020-2271-5 (e-book).

Scarano WR, Messias AG, Oliva SU, Klinefelter GR, Kempinas WG. Sexual behaviour, sperm quantity and quality after short-term streptozotocin-induced hyperglycaemia in rats. Int J Androl 2006; 29(4): 482-8.

Xie BG, Li J, Zhu WJ.Pathological changes of testicular tissue in normal adult mice: A retrospective analysis.Exp Ther Med 2014; 7(3): 654-656.

Konrad RJ, Mikolaenko I, Tolar JF, Liu K, Kudlow JE. The potential mechanism of the diabetogenic action of streptozotocin: inhibition of pancreatic beta-cell O-GlcNAc-selective N-acetyl-beta-D-glucosaminidase.Biochem J 2001; 356(1): 31-41.

Tamas V, Kempler P.Sexual dysfunction in diabetes. Handb Clin Neurol 2014; 126: 223-32.

Argiolas A.Neuropeptides and sexual behaviour. Neurosci BiobehavRev 1999; 23(8): 1127-42.

Jason Gandhi GD, Warren K, Smith N, Ali Khan S.Effect of Diabetes Mellitus on Sexual Arousal and Intercourse. Transl Biomed 2016; 7: 2.

Melis MS.A crude extract of Stevia rebaudiana increases the renal plasma flow of normal and hypertensive rats. Braz J Med Biol Res 1996; 29(5): 669-75.

Shivanna N, Naika M, Khanum F, Kaul VK. Antioxidant, anti-diabetic and renal protective properties of Stevia rebaudiana. J Diabetes Complications 2013; 27(2): 103-13.

Agarwal A, et al.Role of Oxidative Stress in the Pathophysiological Mechanism of Erectile Dysfunction.J Androl 2006; 27(3): 335-347.

Hurtado de Catalfo GE, De Gómez Dumm LNT.Lipid dismetabolism in Leydig and Sertoli cells isolated from streptozotocin-diabetic rats. Int J Biochem Cell Biol 1998; 30(9): 1001-1010.

Author biographies is not available.