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Copyright (c) 2025 Yuanling Zhang, Chao Xu, Fang Li, Guoqing Chen
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.Notch/IL33/ST2 signaling was involved in the maintenance of intestinal epithelial barrier through regulating tight junction after LPS stimulation
Corresponding Author(s) : Guoqing Chen
Cellular and Molecular Biology,
Vol. 71 No. 2: Issue 2
Abstract
Interleukin-33(IL33), an alarm cytokine of the IL-1 family, is expressed mainly in epithelial cells of barrier tissues and is involved in the repair of epithelia to maintain barrier function. However, the mechanisms regulating IL33 expression and the mechanisms by which IL33 regulates the intestinal barrier function are not fully clarified. In this study, Caco-2 cells and siRNA were applied to investigate the role of Notch/IL33/ST2 Signaling in regulating intestinal epithelial barrier function, which was demonstrated by protein expression of tight junctions and trans-epithelial resistance (TER) assay. Our results revealed that Notch signaling pathway was activated and IL33 expression was up-regulated after LPS stimulation. After blocking Notch signaling with DPAT or siRNA for Notch1, IL33 expression was significantly down-regulated in Caco-2 cells. The protein expression of tight junctions (ZO-1, occludin, and claudin-1) was down-regulated after siRNA for IL33 in Caco-2 cells with LPS stimulation. Also, the intestinal epithelial TER was down-regulated after siRNA for IL33 with LPS stimulation or not. Exogeneous IL33 promoted the tight junction protein expression and increased the TER. Finally, our data further showed that IL33 regulates intestinal epithelial barrier function through the ST2 receptor. In conclusion, our results indicated that IL33/ST2 axis, which was activated by the Notch signaling, maintains intestinal epithelial barrier function through regulating tight junction protein expression under inflammatory conditions. This study provides a new therapeutic pathway of regulating intestinal epithelial barrier dysfunction.
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