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Molecular Characterization and TRAP Analysis of Gene in Dendranthema morifolium
Corresponding Author(s) : Y Luo
Cellular and Molecular Biology,
Vol. 61 No. 7: Issue 7
Abstract
The molecular maker system - TRAP was applied to develop a novel and more accurate method to identify the variety and establish the evolutionary relationship of different categories of Dendranthema morifolium. A software - GENESIS 2.4 was used to conduct the cluster analysis and genetic dendrogram establishment. The results showed that 202 different fragments were amplified with 6 pair primers using the TRAP marker system. The polymorphic fragments number is 45, which takes up to 22.3%. The cluster analysis showed that 4 materials used in this study can be classified into 2 main groups and 3 subgroups. The genetic identity is 0.0767 and the average genetic distance is 0.9236 among the four materials. A new tool using the TRAP marker system is more accurate and can be used to identify different categories of Dendranthema morifolium at molecular level.
Keywords
Dendranthema morifolium
TRAP
sequence analysis
molecular marker.
Luo, Y., Li, Q., Wang, X., Yang, F., Nong, S., Li, Q., & Zhu, D. (2015). Molecular Characterization and TRAP Analysis of Gene in Dendranthema morifolium. Cellular and Molecular Biology, 61(7), 119–122. Retrieved from https://mail.cellmolbiol.org/index.php/CMB/article/view/747
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