Issue

Vol. 66 No. 5: Issue 5

The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.

Down-regulation of miR-556-3p inhibits hemangioma cell proliferation and promotes apoptosis by targeting VEGFC

https://doi.org/10.14715/cmb/2020.66.5.34
Wen Jin
Department of Medical Nursing, Tongling polytechnic College, China
Liang Chen
Department of hyperbaric oxygen, The Second People's Hospital of Hefei, China
Fengyun Gao
Department of Medical Nursing, Tongling polytechnic College, China
Mei Yang
Department of Medical Nursing, Tongling polytechnic College, China
Yan Liu
Department of Medical Nursing, Tongling polytechnic College, China
Bo Wang
Department of Medical Nursing, Tongling polytechnic College, China

Corresponding Author(s) : Bo Wang

jia1982yun@163.com

Cellular and Molecular Biology, Vol. 66 No. 5: Issue 5

Abstract

The purpose of this study was to determine the effect of microRNA (miR)-556-3p on cell proliferation and apoptosis of hemangioma-derived endothelial cells (HemECs).  qRT-PCR was used to compare the expressions of miR-556-3p in HemECs and normal cells. The target gene was identified using dual-luciferase reporter assay. Cell proliferation was measured with MTT assay, while western blotting was used to assay VEGFC expression levels. Apoptosis was assayed with FITC Annexin V Apoptosis assay kit. miR-556-3p was overexpressed in HemECs. Transfection with miR-556-3p inhibitor resulted in decreased proliferation of HemECs (p<0.05). The expression of the target gene of miR-556-3pi.e. VEGFC was upregulated when miR-556-3p inhibitor was transfected. The transfection also resulted in increased apoptosis. In HemECs, miR-556-3p is overexpressed and VEGFC expression is low. Thus, miR-556-3p or VEGFC might be potential targets for treatment of angiosarcoma.

Keywords

Angiosarcoma HemECs miR-556-3p VEGFC Proliferation Apoptosis.
Jin, W., Chen, L., Gao, F., Yang, M., Liu, Y., & Wang, B. (2020). Down-regulation of miR-556-3p inhibits hemangioma cell proliferation and promotes apoptosis by targeting VEGFC. Cellular and Molecular Biology, 66(5), 204–207. https://doi.org/10.14715/cmb/2020.66.5.34
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. Boye E, Yu Y, Paranya G, Mulliken JB, Olsen BR, Bischoff J. Clonality and altered behavior of endothelial cells from hemangiomas. J Clin Invest 2001; 107: 745-752.
  2. Mabeta P, Pepper MS. Inhibition of hemangioma development in a syngeneic mouse model correlates with bcl-2 suppression and the inhibition of Akt kinase activity. Angiogenesis 2012; 15: 131-139.
  3. Gnarra M, Behr G, Kitajewski A, Wu JK, Anupindi SA, Shawber CJ, et al. History of the infantile hepatic hemangioma: From imaging to generating a differential diagnosis. World J Clin Pediatr 2016; 5: 273-280.
  4. Walter JW, North PE, Waner M, Mizeracki A, Blei F, Walker JW, et al. Somatic mutation of vascular endothelial growth factor receptors in juvenile hemangioma. Genes Chromosomes Cancer 2002; 33: 295-303.
  5. Lee RC, Feinbaum RL, Ambros V. The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. Cell 1993; 75: 843-854.
  6. Volinia S, Calin GA, Liu CG, Ambs S, Cimmino A, Petrocca F, et al. A microRNA expression signature of human solid tumors defines cancer gene targets. Proc Natl Acad Sci U S A 2006; 103: 2257-2261.
  7. Gordillo GM, Biswas A, Khanna S, Pan X, Sinha M, Roy S, et al. Dicer knockdown inhibits endothelial cell tumor growth via microRNA 21a-3p targeting of Nox-4. J Biol Chem 2014; 289: 9027-9038.
  8. Nakashima T, Jinnin M, Etoh T, Fukushima S, Masuguchi S, Maruo K, et al. Down-regulation of mir-424 contributes to the abnormal angiogenesis via MEK1 and cyclin E1 in senile hemangioma: its implications to therapy. PLoS One 2010; 52: 14334.
  9. Huang C, Huang J, Ma P, Yu G. microRNA-143 acts as a suppressor of hemangioma growth by targeting Bcl-2. Gene 2017; 628: 211-217.
  10. Fu TG, Wang L, Li W, Li JZ, Li J. miR-143 inhibits oncogenic traits by degrading NUAK2 in glioblastoma. Int J Mol Med 2016; 37: 1627-1635.
  11. Kimura Y, Watanabe M, Ohga T, Saeki H, Kakeji Y, Baba H, et al. Vascular endothelial growth factor C expression correlates with lymphatic involvement and poor prognosis in patients with esophageal squamous cell carcinoma. Oncol Rep 2003; 10: 1747-1751.
  12. Xia H, Shen J, Chen S, Huang H, Xu Y, Ma H. Overexpression of VEGF-C correlates with a poor prognosis in esophageal cancer patients. Cancer Biomark 2016; 17: 165-170.
  13. Calin GA, Croce CM. MicroRNA signatures in human cancers. Nat Rev Cancer 2006; 6: 857-866.
  14. Schratt GM, Tuebing F, Nigh EA, Kane CG, Sabatini ME, Kiebler M, et al. A brain-specific microRNA regulates dendritic spine development. Nature 2006; 439: 283-289.
  15. England RW, Hardy KL, Kitajewski AM, Wong A, Kitajewski JK, Shawber CJ, et al. Propranolol promotes accelerated and dysregulated adipogenesis in hemangioma stem cells. Ann Plast Surg 2014; 73: 119-124.
  16. Li D, Li P, Guo Z, Wang H, Pan W. Downregulation of miR-382 by propranolol inhibits the progression of infantile hemangioma via the PTEN-mediated AKT/mTOR pathway. Int J Mol Med 2017; 39: 757-763.
  17. Margileth AM, Museles M. Cutaneous hemangiomas in children. Diagnosis and conservative management. JAMA 1965; 194: 523-526.
Read More
Author biographies is not available.
Crossref
Scopus
Google Scholar
Europe PMC
Download this PDF file
PDF
Statistic
Read Counter : 808 Download : 456

Most read articles by the same author(s)

<< < 1 2